Β-Lactams Lab Report
One of the largest class of last resort clinically used antibiotics worldwide are β-Lactams, such as cephalosporins and carbapenems. β-Lactams inhibit the transpeptidase enzymes which aids in cross linking adjacent peptidoglycans strand. This inhibits the cell wall synthesis which then causes the cell membrane to lysis and causes cell death. They are valued because of their broad activity, limited toxicity and the bioavailability. Unfortunately, bacteria have developed a resistance to the β-Lactams by the expression of the enzyme called β-lactamases, which clinically inactivates the antibiotic by hydrolyzing the four-membered lactam ring of the β-Lactams. It is hypothesises that a peptide bond with Zn2 is broken during a nucleophilic hydroxide …show more content…
This attraction is formed as the hydrophobic regions from each monomer attract together in order to avoid interacting with water.
IV. Active Site
A shallow, broad grove near the end of the β-sheet sandwich is where the binuclear Zn activate site is surrounded by loop 3 (L3) and loop (L10).
The activite-site loop 3 (L3) is flexible as it elongates and disorders the apo form. During the ligand binding, an exteneded beta sheet interaction is formed from b3 and b4. This zippering effect pulls the top of L3 further away from the zinc site and subsequently causes the side chain to reorient away from the zinc center as well.
The zipper effect allows for hydrophobic interactions with the R1 phenyl group of ampicillin to accommdate the substrate. In the L3 loop, resides M67, P68, V73, and A215 are what forms the hydrophobicity of the loop while also hindering the active site less.
Different resides, L65, M67 and W93, form a hydrophobic face and causes reside L65 to move closer to the zinc activte site upon binding of the substrate. This allows for tight hydrophobic interacts with the R1 phenyl group of the
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