Q1A: What is the mechanism of action of colistin? Colistin is an antibiotic that works best against Gram-negative bacteria. It works by binding to LPSs (lipopolysaccrides) and phospholipids in the outer cell membrane of the bacteria. This, in turn, disrupts the outer cell membrane by displacing cations and leaking the intracellular contents, combining it with outer cellular contents, causing the bacteria to be unable to differentiate the bacteria’s intra and outer cellular contents from one another. This ultimately leads to the bacteria’s death. Q1B: What infections are treated with colistin? Colistin is used to treat infections caused by gram-negative or MDRO (Multidrug-Resistant Organisms). Occasionally, professionals will choose to avoid …show more content…
Q2F: Minimum inhibitory concentration (MIC) - Minimum inhibitory concentration is the minimum concentration of the substance necessary to prevent microbial growth. Q2G: Lipopolysaccharide - Lipopolysaccharide is a complex lipid structure containing unusual sugars and fatty acids found in most gram-negative Bacteria and constituting the chemical structure of the outer membrane. Q2H: Enterobacteriaceae - Enterobacteriacaea is a family of gram-negative, anaerobic, rod-shaped bacteria that are usually motile and consist of saprophytes and parasites of worldwide distribution. They can be found in soil, water, plants and animals. Q2I: Mutation - Mutation is an inheritable change in the base sequence of the genome of an organism. Question Set 3: Q3A: The authors hypothesized that colistin resistance was spreading by horizontal gene transfer as opposed to mutation. What specific observation led to this …show more content…
Afterwards, with the use of Glimmer 3.02 and BLAST, gene prediction and annotation were completed with another plasmid, pHN122-1, as a reference. To confirm the role of the gene that caused polymyxin resistance and contained mcr-1, the gene and its sequence were place into a cloning vector pUC18 that yielded pUC18-mcr-1. With this yield and electroporation, it was used to transform an E.coli strain, revealing its ability to confer colistin resistance. Q3D: By what mechanism do the authors propose that the mcr-1 gene confers colistin resistance, and what evidence do they use to support this assertion? The protein sequence of mcr-1 showed its similarity to the polymyxin-producing bacterium, Paenibacillus spp., which showed the possibility of gene transfer occurring. The mcr-1 gene enables protection from polymyxin. The mechanism that the authors proposed on how the mcr-1 gene confers colistin resistance is that mcr-1 causes a modification in lipid A, present in the lipopolysaccharides of most bacteria, which leads to lessened polymyxin affinity. The lipid A has phosphoethanolamine added to it, which in turn, inhibits the bacteria from any attachment. Q3E: What is the origin of the mcr-1 gene, and what evidence do the authors use to support this
R plasmids are responsible for carrying the gene for resistance to antibiotics e.g. ampicillin, which are normally used in the lab. The normal function of a plasmid is to transport genetic information essential to the survival of the bacteria. (Barnnet, 1995). The plasmid can work as vectors for introducing strange DNA. Restriction enzymes are normally used cut foreign DNA and placed it into the plasmid vectors.
They work by either killing the bacteria or retain them from reproducing, with the help of our body’s natural defense system. When bacteria enter our body, our body produces anti-bodies. In other words, they act like soldiers searching for the enemy to destroy, and our bodies are like the army defending us against invasive bacteria. Antibiotics have the ability to recognize “the enemy” because bacteria cells are different from other cells in our body. After the bacteria gets killed, our immune system begins to remove them from the body.
The plate labeled LB: -pGlo had a lawn of bacteria, which meant that all of the bacteria that reproduced was able to survive. The plate labeled LB/amp: -pGlo had no bacteria present at all. This could mean that the -pGlo, the pGlo that received none of the plasmid DNA, had no resistance to ampicillin which would lead to the conclusion that normally, E. coli is not resistant to ampicillin. The plate labeled LB/amp had several colonies of bacteria; this meant that the bacteria that contained the plasmid DNA was able to survive and replicate in the presence of ampicillin. The plasmid DNA is the source of the resistance to ampicillin because that is the only thing different between the LB/amp: -pGlo, which had no bacterial reproduction and the LB/amp: +pGlo, which had a large amount of bacteria survive and reproduce.
It could have also been helpful to show a diagram of how the cell transfers the genetic information containing antibiotic resistance characteristics.
OBJECTIVES To solicit for aid to help study the biological importance of bacillus megaterium and to determine whether bacillus megaterium is susceptible or receptive to antibiotics by investigating the characteristics of the genus species and also. SUMMARY The purpose of this experiment is to be able to identify Bacillus megaterium using 16S PCR & Sequencing and also determine whether bacillus megaterium is susceptible or receptive to antibiotics using gram stain. Being able to determine whether bacillus megaterium is susceptible or receptive to antibiotics is critical to medical advancement and biotechnological enhancements.
[1][2] Bacteria as pathogens Although they are the smallest cells in biocoenosis, their role is even more important. Some bacteria are even able to assimilate (e.g. cyanobacteria or "blue-green algae"). Some of the bacteria decays or rots other organisms to feed themselves. This also benefits
According to the series of test that my group ran for our unknown specimen, we had a match with the bacteria known as Alcaligenes Faecalis. This bacterium belongs to one of the major group of gram-negative bacteria (Phylum Proteobacteria). Alcaligenes Faecalis (Genus, species) is a rod shaped (bacillus), 0.5-1.2 x 1.0-3.0 µm, round with scalloped margin (colony configuration growth), motile (with one to nine peritrichous flagella), gram-negative, non-fermentative bacteria, obligate aerobic, having oxygen as the principal terminal electron acceptor in the electron transport chain (ETC). We consider we have a match with the species Alcaligenes Faecalis because of the following reasons: Fermentation tests performed (Durham sugars) were negative, which indicate that our bacteria use a different metabolic means for growth (non-fermentative gram-negative bacteria).
Introduction In this experiment we are examining Streptococcus Mutans and Lactobacillus Acidophilus which are bacteria found in the mouth. The bacteria Streptococcus Mutans have been seen to have negative effects to the mouth such as tooth decay. Lactobacillus Acidophilus on the other side is seen as a good bacteria and has been used as a probiotic. Both of these bacterias grow within the mouth and can be collected through saliva.
Oxacillin: This antibiotic act to the cell of the bacteria, it block the growth of the cell wall which result in kill the bacteria. Usually it administered by injection. Hypothesis: For mezlocillin I guess it will not work as well as other antibiotics because it has higher chance at gram negative compared to gram positive, which are Micrococcus luteus and Bacillus subtilis. Also penicillin has been used for many year because of it continuously use many bacteria has got resistance to it
Virulence factors are part of an important concept in bacterial pathogenicity (Henderson, Poole & Wilson, 1996). According to Cross (2008), Dubey and Maheshwari (2013), virulence factors enable the bacteria to produce a variety of molecules in order to adhere, invade and evade host defences and cause tissue damage. these molecules have been grouped into adhesins, invasins, impedins, aggressins and modulins (Dubey & Maheshwari, 2013: Henderson, Poole & Wilson, 1996).
L. monocytogenes has pertricous flagella, which allow it to have tumbling motility. One unique property of L. monocytogenes is that it is only motile via flagella in a limited temperature range around room temperature (from between 20 and 25 C). The motility of L. monocytogenes flagella is greatly reduced at body temperature, which is significant because L. monocytogenes is a facultative intracellular pathogen.
Tetracycline which is used to treat acne, respiratory tract infections and other conditions kill bacteria by inhibiting protein synthesis. This is done by stopping molecules to bind on a certain area of the cell called ribosome. Since key molecules can bind to ribosomes which is the site where protein synthesis happens the bacteria will die because it can’t carry out vital functions including asexual reproduction. Some antibiotics kill bacteria by stopping the production of folic acid which is an essential vitamin for proper functioning of the cell’s
The resistant bacteria in animal may expand disease or infection from the contaminated products to humans, or from food handlers and food processors from handling bad meat following resistant bacteria genes to other bacteria, and ending with disease and infection in humans (MSU, 2011). Additionally, the growth also occurs with resistant bacteria from infected animals to non infected animals, through soil, well water, and water runoff from contaminated animal waste to fruits and vegetables or aquatic water eventually to humans (MSU,
The bacteria affects different aspects of its host’s life but especially its reproduction. Depending on
Lactic acid bacteria are also capable of preventing the adherence, establishment, replication, and pathogenic action of certain enteropathogens (Rea, et al. 1996).The