In an effort to showcases how various substances diffuse through membranes, an experiment was performed. Frist, we weighed the mass at the start, to compare it with the weight at the end, and they were expected to gain mass. We left the experiment for a week and left it to diffuse. We then weighed the bags none of them gained mass, which may suggest a random (water stuck in the knot) or systematic (scales) error.
We then tested for the substances outside of the tubing and we were excepting to find glucose and salt outside but not starch as the molecule is too big. Generally, the results proved to be reliable in some aspect, i.e. the expected results of glucose and salt found outside the tubing in the surrounding water, but none of the tubes
Fermentation test is used to determine if unknown #398 uses any oxygen to ferment carbohydrates and acids. Oxidation tests were used to determine if unknown #398 metabolizes carbohydrates and acids by cellular respiration. Both tests are observed by inoculation of unknown #398 into 3 sugar broths: lactose, glucose, and mannitol and 1 citrate (Citric acid) slant. Fifth test, Hydrolytic and Degradative reactions is used to determine if unknown #398 contains enzyme, amylase that hydrolyzes starch after streaking on a starch plate. Next test, inoculation of a urea broth and is used to determine if unknown #398 contains urease that hydrolyzes urea.
These difficulties might have caused me to make mistakes on the amount needed to pipette or the type of substance. Overall, next time I would have made sure to pay closer attention to the pipetting portion of the procedure. Furthermore, we could extend this experiment by trying different kinds of
First, it was hypothesized that test tube "A", the control, would not show any red concentration, test tube "B" which contains supernatant II would show the most red concentration and test tube "C" which contains sediment II would only show a little red concentration. The second hypothesis states that the raw corn kernels would have mitochondrial activity while the boiled corn kernels would not. The last hypothesis interprets that the "gunk" and sediment I will both contain starch granules. It was only expected to find mitochondrial activity in Supernatant II. Unfortunately, after performing this experiment, we were not able to support this hypothesis and come up with a conclusion.
To begin, one must test for monosaccharides. Glucose is necessary, and is needed to be placed into a test tube at a quantity of 5 mL. 3 mL of Benedict’s solution is then added. The test tube is then placed in a beaker of boiling water for five minutes or until the color changes. If the color changes, then it is known that monosaccharides are present in the solution. Next, one will test for starches.
The investigation was carried out to identify the presence or absence of biological molecules in serum 2216. If the concentration in each test tube of the dilutions carried out will be more concentrated then the concentration of the test tube before it, then the color will be at an equal concentration with the other dilutions performed. The hypothesis was wrong because of the difference in concentrations due to the different measurements within the dilutions done. The test for starch was to add a drop of iodine solution to the pipette in the spotting tile. A reducing sugar solutions is add inside a test tube with 3 drops to then add 3 drops of benedicts and plane in a water bath.
Results and Observations for Organic Macromolecule Presence Tests Test for Lipids Test for Proteins Test for Glucose Test for Starch Observations: Observations: Observations: Observations: Paper-bag has a translucent spot The stomach contents became purple The stomach remained blue The stomach contents became black Present? Yes Present? Yes Present? ___ Present?
The iodine test determines the presence of starch in biological materials. It is predicted that, if starch is not present, the solution with iodine remains yellow. However, if starch is present the solution with iodine becomes a blue-black colour. Plants have starch as the storage polysaccharide (glucose units held together by glycosidic bonds) while animals have the equivalent of glycogen. In this experiment, the dark blue colour is visible because of the helical amylose and amylopectin reacting with iodine (Travers et al., 2002).
In this practical agar jelly cubes will be used to represent a cell. AIM: To model diffusion in a practical form and investigate the effect of surface area to volume ratio. HYPOTHESIS: It is hypothesised the smaller the cube the quicker and bigger the rate of diffusion will be and with a larger cube there will be a smaller percentage of diffusion due to its bigger volume.
This experiment was created to contrast the effects of osmosis between three gummy bears in tap, sugar, and salt water. To compare the gummy bears, three cups were gathered and filled with twenty-five milliliters of the particular water solution. Then the mass and volume of each gummy bear was recorded into a chart. To find the mass, zero a balance once a strip of wax paper is placed over, and then place your gummy bear and record the mass. To find the volume of the bear, multiply the length, width, and height of the bear in centimeters with the help of a ruler.
Another five tea bags were soaked for fifteen seconds in beaker B and then removed. The same teabags were then placed into beaker C for two minutes. They were removed after the time elapsed. 4. The solutions were allowed to cool to room temperature using an ice bath.
5 water bath were set up each to10 °C. (5 were used do the experiment faster) 5 cm3 of starch solution were added into the 5 test tubes that were labeled test tubes. Then 5 cm3 of amylase enzyme was added into the other 5 test tubes that were labeled. Put one of the starch solution test tube (preferably the one labeled 1) and one of the test tube containing amylase into the water bath (10 °C).
The hypothesis we came up with for this project was that in the distilled water there wouldn't be no change in the potato, we wouldn’t see a gain or loss with the water sitting in the beaker. The beaker with the 30% Sucrose and Distilled water we predicted that there was going to be weight gain to the potato. And for the distilled water with the 30% sucrose and we predicted weight loss. But the results came out to be the first bag content being isotonic the second bag came out to be hypotonic and lastly the result came out to be
Also, although this likely served no contribution in disheveling the results, using a stirrer of the same material to ensure the separate testing of each substance will be as uniform as
The experiment shall use several concentrations of sucrose solution and a substance known as Methylene blue. A piece of potato/ carrot shall be placed in a boiling tube and the solution shall be poured into it. This tube shall have Methylene blue added into it. After incubation some of this solution shall be taken out with a pipette and inserted into a separate boiling tube containing the same sucrose solution however this solution shall be known as the pre-incubated solution. The drop shall be watched so as to see if the density of the water and concentration of sucrose has increased or not, displaying the water
It justifies the idea that increasing the concentration of sucrose does in fact speed up the rate of osmosis, an therefore increase the mass of the Visking tube. This happens due to the water molecules moving from a high water concentration to a low water concentration. Another reason why this hypothesis is correct is because sucrose particles are too large to go through the membrane therefore the sucrose does not diffuse into the water. According to the mean graph there is a clear outline that indicates constant acceleration, which justifies that the increase of the independent variable affects the dependent