Maia Naylor and Margot Cathelin
10/21/2015
Grade 9
Enzyme Lab Report
Purpose:
Which temperature of potato (freezing, room temperature, boiling) when added to hydrogen peroxide, would produce the most enzyme activity?
Research Question:
How does hydrogen peroxide break down enzymes?
When hydrogen peroxide and enzymes meet, they instantly form a reaction. This reaction is the chemical distillation of enzymes. The reaction is enzymes breaking down and turning into bubbles.
How does temperature affect enzyme activity?
Potatoes have enzymes in them. Enzymes are catalysts, which means they speed up chemical reaction. This chemical reaction produces oxygen gas. In hot substances, the enzyme reaction speeds up. In cold substances, the reaction takes a long time, or will never create a chemical reaction
Does boiling a potato eliminate its enzymes?
Boiling a potato will speed up its enzyme chemical reaction, but if you heat it up to much it will cook and the reaction will stop because all of the enzymes will be cooked.
Does freezing a potato eliminate its enzymes?
Freezing a potato will not eliminate its
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Having different temperatures of potatoes would help us see which one of three potato temperature would produce more enzymes. The dependant variable was the the amount of activity (bubbles) produced. Measuring this would help us determine which temperature of potato produced more activity. The potato with the most bubbles, the room temperature potato, would be the one with the most enzyme activity. There were many controlled variables. Some of them included temperature of hydrogen peroxide, amount of hydrogen peroxide and the amount of potato. If any of these components were off place, than the whole experiment would be rigged. The factors needed to stay the same throughout the different potato types because then we could base our answers purely on one factor, rather than an unfair
As pH increases or decreases to get closer to the optimal pH --in this case it is 7 for this particular enzyme-- the rate of reaction peaks and is highest at that point, which is described by the molecular shape and structure of the enzyme at its optimal pH. When turnip peroxidase is at pH 7, the active site is able to fit perfectly with the substrate, therefore explaining why the reaction rate is fastest at this point. Accordingly, if the active site is disrupted, the substrate cannot fit perfectly causing the reaction rate to slow down. This can be supported by the data because the reaction rate gradually increased from pH 3 to pH 7 and reached its maximum at pH 7. Once it did reach the optimal pH, the reaction rate continuously decreased
Enzymes are a form of protein that lowers activation energy and speeds up reactions as a catalyst. They are made by the stringing together of an abundant amount of amino acids and folded into a specific shape for chemical reactions. Turnip Peroxidase is the enzyme used in this lab and is derived from the vegetable. Enzymes are not used up or permanently altered by their environment Peroxidases are found in a range of organisms and function to break down alcohol (H2O2) and creates byproducts of oxygen and water. In this experiment, the reducing agent guaiacol is added with the substrate, hydrogen peroxide, to create water and oxygen.
The effect of pH on the speed of enzyme interaction with substrate chemicals Hypothesis: About pH: If the pH level is less than 5, then the speed of the enzyme reaction will be slower. About temperature: If the temperature stays the same, then the speed of the enzyme reaction will not be completely affected. Background information: The function of enzymes is to speed up the biochemical reaction by lowering the activation energy, they do this by colliding with the substrate.
Exploration Title: Effect of Temperature on rate of Osmosis Submitted By: Abdulkarim Kamal Date Submitted: October 19th 2015 Subject: Biology HL Teacher: Mr. Nick Aim: This is an investigation to determine the relation between temperature of a solution (sucrose) and the rate of osmosis Scientific Context: Osmosis is defined a passive transport process in which a fluid diffuses across a semi-permeable membrane, from an area of high solute concentration to an area of low solute concentration and vice-versa. There are various factors that could potentially influence the rate of osmosis; these factors include volume, concentration, and temperature. If all external factors that may interfere with rate of osmosis are controlled, the results will show equal amounts of fluid on both sides of the barrier (membrane); this is known as an “isotonic” state.
3. Look at your graph for Part B, how does temperature affect enzyme activity? The colder the temperature the greater the reaction. 4. Look at your graph for Part C, how does pH affect the enzyme activity?
Independent Variable amount of substrate (sucrose) present 3. Controlled Variables temperature, pH, sucrase + sucrose incubation time 4. Describe what is measured as an indicator of sucrase activity and why this is an indicator of sucrase activity. I believe glucose and fructose was used as an indicator because they are what produces sucrose and sucrose creates more sucrase activity.
Independent Variable pH 3. Controlled Variables temperature, amount of substrate (sucrose) present, sucrase + sucrose incubation time Effect of Temperature on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2. Independent Variable temperature 3. Controlled Variables pH, amount of
Introduction: Enzymes are biological catalysts that increase the rate of a reaction without being chemically changed. Enzymes are globular proteins that contain an active site. A specific substrate binds to the active site of the enzyme chemically and structurally (4). Enzymes also increase the rate of a reaction by decreasing the activation energy for that reaction which is the minimum energy required for the reaction to take place (3). Multiple factors affect the activity of an enzyme (1).
Enzymes are proteins that catalyze chemical reaction, and they work best at their optimal conditions (optimum pH, temperature etc.) but when the environment is not close to the optimum conditions, the enzymes denature and do not function anymore1. An excellent example would of the effect of temperature on yeast fermentation would be that the bacterial cells if exposed to very high temperature (above the optimal) would no longer function since their enzymes are denatured. The yeast would produce the most Carbon dioxide in the optimal temperature (45 °C ±1/°C) and other temperatures below the optimal temperature would not produce sufficient Carbon dioxide and any temperature above will produce too much that it will lead to the sinking of the bread and death of yeast because its enzymes have been denatured, therefore the reaction will stop. The bread will certainly sink if is not exposed to the right temperature the yeast will not ferment
By using the same mass of potato slices and putting them in different concentration of solutions for a specific amount of time will tell us how the concentration changes the mass of the potato slice. Therefore changing the rate of osmosis. Hypothesis: I predict that, if the piece of potato was put into a solution that has a high concretion of sucrose then the potato slice would lose mass as it would lose water from its cells because the water is moving out of the cell from a high concentration to a low concentration of water through a semi- permeable membrane. The cell is hypotonic and the solution is hypertonic.
In this experiment , we can prove that the temperature, pH and salt are the factors that will affect the structure and function of the enzyme as it is a kind of protein . Therefore, there may be an influence on the activity of enzyme which substrates cannot be binded on the active site if the amylase in too high or low ph and temperature and excess salt environment . On the other hand optimum ph and temperature and suitable salt concentration may favour the amylase activity . Reference : 1.2016, May 08). Effects of pH on Amylase Activity.
Uncontrolled Environmental conditions Atmospheric conditions The controlled variable Concentration of amylase was kept under control by measuring the amount of amylase used and also it was made sure the percentage of amylase used was 1%. The Amount of amylase/starch used were kept to 5cm3 at all times. Materials needed Beakers Bunsen burner Test tube Thermometer Stopwatch Test plate Glass rod Starch Amylase solution Water bath Iodine solution. Test tube holder Labels Marker Procedure First 5 test tubes were taken and labeled with numbers from 1 to
Catalase and Temperature Introduction Background: Enzymes are catalysts which help reactions inside of organisms such as cells. Many different types of enzymes are used to catalyze different types of reactions. Enzymes are able to catalyze reactions that normally wouldn’t be possible under the specific circumstances in the cell such as the pressure or temperature of the cell. The way an enzyme works is it binds with the active site of a substrate and creates an enzyme substrate complex. The enzyme then breaks apart the bonds in a substrate and then leaves unchanged after the reaction.
Along with being found in plants, they are also present in liver cells, kidney cells, leukocytes and erythrocytes. For the concentration of enzyme experiment, the hypothesis was if the concentration of an enzyme increases, then the enzyme activity will increase as well. The hypothesis was proven to be true, because there are more enzymes to react with substrates. For the enzyme—factors affecting, the hypothesis concluded was if the temperature increases, than the enzyme activity will increase. This however was proven wrong, because enzymes become unstable at higher temperatures.
Catalase Enzyme Lab: Research Question: What is the impact of the temperature (of a potato) on the rate of reaction (measured by the amount of O2 bubbles formed)? Background Information: Enzymes are proteins that aid certain chemical processes that take place. When a chemical reaction takes place, a certain amount of energy is need for it to occur. When an enzyme is present, the amount of energy needed for a chemical process to occur is reduced.