The materials and methods used in this experiment are as written from the Laboratory Supplement of David L. Shultz. To properly prepare for the experiment, the materials needed were one stoppered shell vial or small Erlenmeyer flask for flies, seventy flesh flies (Sarcophaga bullata, ice-cold to anesthetize them), one wire test-tube rack (small mesh), a few paper towels to cut the flies on, one razor blade, one enamel pan, one thermometer, one grease pencil, one piece of plastic wrap, one large plastic beaker filled with crushed ice, seven large glass test tubes to hold reagents, homogenate, and centrifuged fractions, seven glass reaction tubes (small size, about three inches tall), and seven plastic pipettes (one milliliter and controls). When all …show more content…
Fifteen milliliters was transferred to a clean tube marked H, for whole homogenate, and kept on ice at all times. The remaining fifteen milliliters of homogenate was transferred to a clean centrifuge tube and placed in a beaker of crushed ice. A balance was prepared by putting fifteen milliliters of distilled water into a new centrifuge tube and both tubes were then placed into the refrigerated centrifuge. It was then centrifuged at 5000 rpm for twenty minutes. When the centrifuge stops, the tube containing the homogenate was retrieved and held at the same angle. The supernatant was poured into a clean, rinsed with distilled water, twenty-five milliliter graduated cylinder without pouring out the pellet. The volume of the supernatant was restored to fifteen milliliters with the homogenizing medium, shaken well and transferred to the clean tube, marked S, and kept on ice. The volume of S was exactly the same as H. Ice-cold homogenizing medium was added to the pellet to make the final volume exactly equal to the original volume centrifuged, fifteen milliliters. The tube was stopped and shaken to suspend the
Cadet Eric Wiggins Date: 18 September 2014 Course Name: Chem 100 Instructor: Captain Zuniga Section: M3A Identification of a Copper Mineral Intro Minerals are elements or compounds that are created in the Earth by geological processes. The method of isolating metals in a compound mineral is normally conducted through two processes.
The serial 2-fold dilution were done with a volumetric pipette, its pump, and 10 mL volumetric flasks. Eight different solutions were produced, half of which came from Red 40 and the other half, from Blue 1. These different concentrated solutions were placed in a 10 mL volumetric flask, each labelled with either R for Red 40
ABSTRACT NRC-04, a novel antimicrobial peptide derived from skin mucous secretions of flat fish winter flounder, shows a broad spectrum of antimicrobial activity. In order to understand the conformational change of NRC-04 in different types of membrane, our team did experiments on NRC-04 with negatively charged bacterial surface membrane mimetic micelles sodium dodecyl sulphate(SDS), zwitterionic eukaryotic middle membrane mimetic micelles dodecylphosphocholine(DPC), gram-negative bacteria outer membrane mimetic micelles Lipopolysaccharide(LPS) and bacterial inner membrane mimetic micelles 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol(POPG). Fluorescence test shows that the C-terminus tryptophan residue of NRC-04 interacts with the hydrophobic
The lab started off by measuring critical materials for the lab: the mass of an an empty 100 mL beaker, mass of beaker and copper chloride together(52.30 g), and the mass of three iron nails(2.73 g). The goal of this experiment is to determine the number of moles of copper and iron that would be produced in the reaction of iron and copper(II) chloride, the ratio of moles of iron to moles of copper, and the percent yield of copper produced. 2.00 grams of copper(II) chloride was added in the beaker to mix with 15 mL of distilled water. Then, three dry nails are placed in the copper(II) chloride solution for approximately 25 minutes. The three nails have to be scraped clean by sandpaper to make the surface of the nail shiny; if the nails are not clean, then some unknown substances might accidentally mix into the reaction and cause variations of the result.
Genetic engineering is changing the DNA code to express different traits. A plasmid is a circular piece of DNA that contains important genetic information. Recombinant DNA is the product after inserting your desired genes. The genes we hoped to insert in the pGLO lab were the GFP gene and the ampicillin resistance gene. GFP was needed so that we would tell if the ampicillin resistance gene had been properly placed when the bacteria glowed under a UV light.
When using a hypertonic and hypotonic solutions they can be shown in many different ways one way we showed this is putting an egg in corn syrup. The purpose of this lab is that students can observe and predict what is happening to an egg when you put it in hypertonic and hypotonic environments. The environments we put the egg in are vinegar, corn syrup, and water with food coloring. Using these materials we learned about osmosis, hypertonic environments, hypotonic environments, and passive transport. The purpose of this lab was to learn and get a better understanding of what is happening in the movement of molecules.
Procedure The various solids and liquids were used for this lab. The first experiment had effects of the temperature towards the chemical reaction. The first experiment had two test tubes filled with same amount of substances to react, but one was at 10 ºC while the other one was at 50 ºC. The second experiment involved different molar concentration of substances reacting. Individual test tube was filled with 1.0,
After obtaining an homogeneous mixture, the flask was placed in an ice bath during five minutes next to a graduated cylinder containing 5.0 mL of concentrated sulfuric acid. The temperature of the ice bath was recorded to be 1.1 °C. Likewise, a second graduated cylinder containing 1.8 mL of nitric acid and 2.5 mL of sulfuric acid was immersed in the cold ice bath to keep the three different solutions at the same temperature. Thereafter, the cold 5.0 mL of H2SO4 were added to the erlenmeyer flask containing the acetanilide solution, which remained in the cold water for approximately another 4 minutes.
Glacial acetic acid and acetic anhydride were added to the mixture while refluxing, which converted the lime colored solution into a clear mixture. The flask was cooled in an ice bath and the solution
Elijah Brycth B. Jarlos IX-Argon 1. Multicellularity is a condition of an organism to have multicellular cells. An example of a organism who has multicellular cells are plants, animals, and humans. The main reason of why scientists have a hard time finding a good set of existing organisms to compare. Is neither the first set of organisms which is being compared is dying as fast as the second specimen is being examined or they just can’t find the right species.
Title: THE BALLOON INFLATION REACTION Introduction: Chemistry is one thing that makes us understand and gives us reasons of why certain reactions gives certain results. In this experiment we will be illustrating the reaction between baking powder and vinegar and see what happens to the balloon that is attached to it. Hypothetically the reaction of the vinegar and baking powder will produce carbon dioxide which will inflate the balloon. If the more vinegar may happen that when more vinegar is added to the baking powder it may produce more carbon dioxide thus the balloons diameter increases.
A scale of zero to five was used to describe the reactions, with zero being no reaction at all, one being a slow reaction, and five being a very fast reaction. The materials used were a test tube rack, six test tubes, a test tube clamp, forceps, a graduated cylinder, four small pieces of liver, one piece of potato, one piece of hamburger meat, approximately forty milliliters of hydrogen peroxide in a forty milliliter beaker, a splint, and matches. An ice bath and boiling water was required for testing, where a hot plate was used to boil the water. Each test tube given a label, which were “cold”, “room”, “hot”, “warm”, “potato”, “meat”, and
In this experiment three of the system variables, water temperature, air temperature, and air flow, were changed and compared to a base trial, Trial 1. The variable that effected the system the greatest was temperature. The data collected from this experiment indicates that the most efficient cooling occurs when there is a larger difference between the water and air inlet temperatures. Trial 1, heated water with unrestricted air flow at room temperature, had the largest change in temperature of the water at 2.8⁰C. Additionally, this trial had the best closure of the energy balance by approximately 15%. Trial 3, which had heated air and water with unrestricted air flow, had the worst closure of all of the trials, and the second worst temperature
II. METHODOLOGY In order to perform this experiment, the students will need a distillation set-up with a connector receiver, an iron ring and stand, a Bunsen burner, a wire gauze, a 250mL round bottom flask, a graduated cylinder, a thermometer, one or two boiling chips, an alcoholic beverage, masking tape, an ice bath, a stirring rod, and, optionally, food coloring. It is imporatnt to avoid playing with the apparatus and equipment so as to avoid breakage and injuries, especially since fire is being dealt with in this experiment.
The second technique is moist heat sterilisation whereby an autoclave is used. Autoclave is operated at a temperature of 121°C and a pressure of 15 psi for about 15-20 minutes. In this experiment,