The objective of the sludge lab was to determine how many different pure substances were in the sludge by using the methods and techniques we have learned throughout the year. We had to pick separation methods so we could separate our sludge and then test characteristic properties on our separated liquids and solids. This experiment made us use our knowledge on characteristic properties to pick the ones we should test to help us identify our pure substances. Characteristic properties are properties that help identify a solid or liquid. Each solid or liquid has a certain density, boiling point, solubility, flammability, so if you know what each one is then you can use that information to help you identify your solid or liquid. My partner and I …show more content…
Based on our initial observations(appearance) of solid #4 and the fact that they are not soluble in water and will sink, my partner and I have concluded that solid #4 are small pebbles. Solid #2 are small pieces of tin foil. We believe this because solid #2 isn’t soluble in water and doesn’t float just like tin foil. We also think this because of our initial observations(appearance). Nika and I believe that solid #6 is dried up purple food coloring because once you put the solid in water it dissolves and the water becomes purple(just like the color of our sludge). When we collected liquid from our distillation separation method, liquids #5 and #4 came out clear (without the food coloring). We believe solid #6 is what made our sludge purple. The density of food coloring is the same as water: 1 gram per square millimeter, our density was very close to this it was 0.51g/cm3 and we could have made mistakes when reading the graduated cylinder. Solid #5 is silica sand based on the fact that it isn’t soluble in water. We also used our international observations(appearance) and agreed that it was a match. Even though the density of silica sand is 2.65g/cm3 and ours was 1.3g/cm3 we agreed that we
Identification of bacteria within Unknown Culture #21 In this experiment, an unknown culture of two different types of bacteria was assigned to each person, a number of tests were performed to isolate and identify these bacterial cells. Based on knowledge from the previous experiments completed in lab, a basic understanding of each type of bacteria was used to create a flow chart that would aid the process of identifying the unknown bacteria within the culture. A gram stain that is performed initially will narrow down the types of tests certain bacteria will and will not respond to. In addition to the gram stain, some of the tests that were used include, a catalase test, an Eosin methylene blue (EMB) agar test, a bile esculin test, and a 6.5% sodium chloride (NaCl) test.
Chemical Reactions and Identifications of Unknowns Data Analysis Name: _Gloria Smith_________________________________________ Please answer the following questions with complete sentences unless a fill in the blank is given. Your answers must be typed. Do not plagiarize! Identification Tests: Flame tests are used to identify the __metal ions_ of a compound. Litmus paper is used to identify acids and bases.
Characteristic Property- Test 2- Density Materials: Triple Beam balance, distilled water, graduated cylinder, unknown 6 Procedure: first we found the mass of the empty graduated cylinder and then its mass with the now distilled unknown. After subtracting the mass of the graduated cylinder, we were able to find the volume. For every 1mL=1cm³ so there we had the volume found with the graduated cylinder. We divided the mass by the volume in order to get the density Data: We found that the density of our unknown was 0.76 g/cm3.
Introduction- We did an experiment about different types of movements called kinesis and taxis. Kinesis is the change in the speed of movement or the rate of turning which is intensified by a stimulus (Meyer, 2006). Taxis is movement towards or away from a stimulus (Meyer, 2006). We did our experiment using Sowbugs.
Purpose: To identify an unknown microorganism by performing a series of biochemical tests on a pure bacterial culture. Materials and Methods: Tests: Lactose fermentation: Fermentation makes energy available for use by microorganisms by anaerobic breakdown of carbohydrates. The product can either be an acid or gas. When it is positive, the broth will turn from red to yellow and if gas is present a bubble is formed.
Unknown Lab Report Unknown # 25 By: Jenna Riordan March 19, 2018 Bio 2843 1. Introduction Microbiology is the study of microorganisms found in all different environments throughout Earth, from the hot thermal vents at the bottom of the ocean to the ice at the top of a mountain.
3mL of the liquid in each of the vials were added into cuvettes and measured in the spectrophotometer. Before each time point the photo spectrometer was zeroed using a cuvette with 3mL of distilled water. If any of the results were considered unusual the machine was zeroed again and the sample was retested. The results from the spectrophotometer test were recorded in a table. The experiment was repeated six times to gain a sample size of six.
Morgan Spurlock did this experiment because two overweight girls were sewing the Mcdonald 's fast food restaurant for making them obese. The girls lost the case because they couldn’t prove that Mcdonald’s food made them obese. Morgan did this experiment to prove if Mcdonald’s food did have an effect on your health after eating only Mcdonald 's. During the experiment Morgan had to follow rules and guidelines. Rule #1 is that when only when asked Morgan must eat Super Sized Meals.
Utilizing the method that the experimenters have come up with, the procedure isolates the variable being tested, which is the paper towel brand. By not changing other aspects of the procedure, other factors have a lower chance of affecting the accurate results of the answer to the main question. Additionally, the method is a quick and simple experiment, causing no use of excessive time than the provided duration. Within the first minutes of the experiment, the experimenters and the advisor realized that the method did not really provide accurate results due to merely estimating the numbers without the use of a graduated cylinder. Changing that aspect of the procedure by using a graduated cylinder to measure the amounts of water instead helped
Bacterium growth on various agar plates Introduction The purpose of this experiment is to show different agar plates inhibit or enable growth of different organisms. Some varieties of media enable the grow of a wide range of organisms such as nutrient agar. Other media are selective which means they contain specific nutrients to encourage the growth of certain organisms. This means other organisms will die due to the selective nutrients such as high concentration of salt which will cause plasmolysis.
Rediet Legese iLab Week # 6 CRUDE OIL DISTILLATION Introduction: The aim of this week lab experiment is to experiment distill crude oil and to check how temperature determine the chemical properties of crude oil plus how the boiling point can also show physical properties. They are two major finding in this experiment. he first finding was the point at which the raw petroleum is heated to the point of boiling, at 275 0C, the gas and kerosene oil are refined, however the oil (lubricant ) stays as an unrefined feature oil.
The wax melted first, followed by the salt, and lastly the sugar. The salt not only burned before the sugar, but developed a brown color throughout most of the substance at a faster pace. In the water solubility test, the salt dissolved in the water, as soon as it was properly mixed and the sugar dissolved in the water as well, but was stirred for a longer period of time until it was completely dissolved. The wax did not dissolve in the water, however bits of the substance broke off from the main piece. With this information, the final results included: wax as a nonpolar covalent compound, since the substance melted first and was not soluble in water; salt as a polar covalent compound, since the substance was soluble in water and the second to melt; and sugar as an ionic compound because the substance melted last and was soluble in
Joshua Miller 12/18/17 Fermentation Lab report Introduction The term fermentation refers to the chemical breakdown of a substance by bacteria, yeasts, or other microorganisms, typically involving effervescence and the giving off of heat (wikipedia). Sugars are converted to ethyl alcohol when fermentation happens. In this experiment we determined if yeast cells undergo fermentation when placed in a closed flask with no oxygen. Glucose and yeast are mixed together in a closed flask and allowed to incubate for about one hour.
Experiment #7: Column Chromatography of Food Dye Arianne Jan D. Tuozo Mr. Carlos Edward B. Santos October 12, 2015 Abstract Column chromatography is the separation of mixture’s components through a column. Before proceeding with the column chromatography itself, a proper solvent system must be chosen among the different solvents. The green colored food dye is the mixture whose components are separated.
Introduction The term chromatography actually means colour writing, and signifies a technique by which the substance to be examined is placed in a vertical glass tube containing an adsorbent, the different segments of the substance traveling through the adsorbent at distinctive rates of velocity, according to their degree of attraction to it, and producing bands of colour at different levels of the adsorption column. The substances least absorbed emerge earliest; those more strongly absorbed emerge later. (Wixom et al., 2011) In chromatography of all types, there is a mobile phase and a stationary phase.