Unknown #10 produced no identifiable macroscopic characteristics as a broth, so the first step was to Gram stain a loopful to determine the microscopic characteristics. Gram staining not only helped identify Unknown #10’s microscopic morphology but it also helped ensure the specimen was a pure culture—no other bacteria were visible when Unknown #10 was Gram stained and observed under the microscope. Unknown #10’s key microscopic morphology was that it was a very small, Gram negative bacillus. Though bacilli can possibly form endospores, no empty white centers were visible which suggested that Unknown #10 was not an endospore forming bacteria. No quick endospore stain was performed to validate this assumption since only one assigned organism was endospore forming and unlike Unknown #10, that organism was Gram positive. By Gram staining alone, it was safe to eliminate the three Gram positive bacteria that could have been assigned: S. epidermidis, M. luteus, and B. megaterium. The second step was to streak plate Unknown #10 to observe its macroscopic …show more content…
Starch amylase testing was equally unsubstantial since the only amylase producing bacteria was ruled out after Gram staining. Unknown #10’s negative citrate test result was also unhelpful because E. coli is citrate negative and P. vulgaris is a variable citrate producer that can also be citrate negative. H2S production in the Kligler’s Iron Agar test ultimately proved that Unknown #10 was Proteus vulgaris. P. vulgaris is the only assigned bacteria that produces H2S, so when a black precipitate obscured the yellow butt of the Kligler’s Iron Agar slant, E. coli was ruled out. Not only did the H2S product confirmed that Unknown #10 was P. vulgaris, it confirmed P. vulgaris’ motility. Only a motile bacteria could travel that far from the center of inoculation. Furthermore, it confirmed that P. vulgaris is a facultative
Methods Unknown microbial #398 went through several of tests in order to identify its characteristics when isolated from a urine sample of Doris, a 64- year old patient with a kidney infection. To identify unknown #398, must prepare a working and a reserve stock by the inoculation from a broth culture and by quadrant streaking method on a PEM and EMP plates. The following test procedures were incubated at 37°C for 48 hours for observation and identification for unknown #398. The identification of unknown #398 followed test procedures from Brown1.
Chris McCandless was an American adventurer who traveled to the Alaskan wilderness in April 1992. He look little food and equipment with him, before embarking on his journey McCandless abandoned his car and burned his money. He wanted to live simply in solitude away from the materialistic world. Timothy Treadwell, an environmentalist, also ventured to Alaska to study grizzly bears. Both Chris and Timothy set out to explore what the wilderness had to offer and they didn’t let anyone stop them from doing what they desired.
Unbroken is a book written by Laura Hillenbrand. The book is about Louie Zamperini. Throughout his childhood, he was always a troublemaker. He stole, he lied, and wreaked havoc in his town. In highschool he decides to do track and dedicates all his time to it.
The memoir of Night is a powerfully emotional experience that the Jews endured, that also impacted the world. Elie Wiesel writes about his struggles and living the life of Jews in the concentration camps Bierkenau, Auschwitz and Buna. Elie writes with such meaning, the reader can feel his emotions. Jews were killed off, as Hitler believed Jews shouldn’t have existed in this world. The power of Elie Wiesel’s moving story helps people to really understand what torture the Jews went through and how they were forced to live.
The Holocaust changed the lives of many people. Over eleven million people were killed during this tragic time in history, six million were the Jewish people. The small percentage of those who survived were terrified to tell their stories and relive through the horrendous moments of the past. Elie Wiesel was able to overcome this fear and publish a candid and horrific autobiography illustrating his survival as a teenager in the Nazi concentration death camps. Elie Wiesel's Night is a powerful and moving story uncovering the truth of when life is reduced to the essential struggle to survive, insensitivity and cruelty can occur.
Over the last few class periods, our Theory of Coaching Soccer class viewed the documentary Class of 92. This film focuses on and showcases six young soccer-playing men who would go on together as members of the Manchester United to conquer the world in the arena of soccer. The Class of 92 follows (as a reenactment) the journey of these six young men (Paul Scholes, Ryan Giggs, David Beckham, Nicky Butt, and Gary and Phil Neville).o This film begins with a nostalgic scene of the soccer players intently listening in on a game they played in on May 26, 1999. After the opening scene, the film sets the stage for following each player and their individual journey as a part of the team and a part of the Manchester United.
Unbroken is a biography by Laura Hillenbrand about Louie Zamperini’s entire life. Louie Zamperini is a son of Italian Immigrants that moved to Torrance, California. He starts out as a problem child that would steal, drink and smoke, but he eventually straightens out through sports and became an Olympian later in life. After he went to the Olympics, he gets drafted into World War 2.
The Horrors of the Naoetsu POW Camp Throughout Laura Hillenbrand’s Book Unbroken: An Olympian’s Journey from Airman to Castaway to Captive, Louie is sent to numerous POW camps but eventually ends up at the Naoetsu POW Camp, where he is finally liberated. This camp, the last that Louie has to endure, does not come without struggles and beatings that can put anyone over the edge. Although all parts of the Naoetsu POW camp were horrible, the worst parts were the dreadful living conditions, a wretched prison guard, and extensive torture sessions that caused men to lose all hope of survival. Living conditions at the Naoetsu POW Camp were deplorable and unsanitary, causing Louie’s mental health to decline and his life to worsen. On page 201 of Unbroken,
Each and everyone has our own idea of how success looks like. But for me, success is something that you’ve worked so hard to get to that you now have pride to have or be. But success isn’t so easy to reach. There's going to be challenges and times where one thinks they won't succeed. Its when you’ve flourished to the point where you’re happy with the placement of your life.
Jack Ma once said, “The world needs new leadership, but the new leadership is about working together.” This could not be more true at Boston University through its Kilachand Honors College. I believe it is because their approach of interdisciplinary problem-solving, is about expanding students’ world-views. In this program you are learning with different individuals who have different interest and fields of study. According to Pew Research Center, “Political polarization is the defining feature of early 21st century American politics...”.
For this exercise, I chose to break the norm of walking on the right side of the hallway and using the right door while entering and exiting a building. This norm has been formed to keep order in crowded hallways and avoid running into other people while walking from place-to-place. This norm upholds the values of creating order in crowded spaces, as well as obeying people’s personal space and boundaries. This norm is common in American society because it assures that people walk in an organized fashion and can get where they are going without running into others. When this norm was broken, the flow of traffic in the hallways and stairwells was interrupted and it was more difficult for people to get where they were going in an efficient manner.
The sterile cotton swab was inserted in the S. epidermidis culture and twirled around to obtain a specimen. The entire plate was inoculate with the swab from top to bottom, to achieve a lawn of growth. The dry forceps was used to remove the antibiotic disk into the appropriate spot on the plate. This process was repeated for the all antibiotics with aseptic technique being used. The plate was incubate with lid up on the bookshelf at room temperature for 48 hours.
Investigate New York Institute of Technology—a dynamic, very positioned, and licensed not-revenue driven college focused on instructing the up and coming era of pioneers, and to moving advancement and propelling business enterprise. Our 12,000 understudies from about every one of the 50 states and 100 nations at grounds the world over get to be locked in, mechanically sharp doctors, designers, researchers, engineers, business pioneers, computerized craftsmen, social insurance experts, and that's only the tip of the iceberg. NYIT at a Glance
The setting of the novel Beloved was the reconstruction era in 1873, it focused on the powers of memory and history. The past was a burden that the former slaves were desperately trying to get over. To Sethe the protagonist of the novel, her memories of slavery were inescapable. Her memories continued to haunt her. Eighteen years earlier Sethe murdered her daughter, she slit her throat in order to save her from a life of slavery.
The title of my book is Beloved, by Toni Morrison. The physical setting is a haunted house in Kentucky. The temporal setting is in the late 1860s, during a time of slavery. The author uses flashbacks to manipulate time. The setting is critical to the story.