The Effect of Sugar Concentration on CO2 Production by Cellular Respiration in Yeast Introduction In this lab, our main focus was to find how sugar concentration affect yeast respiration rates. This was to simulate the process of cellular respiration. Cellular respiration is the process that cells use to transfer energy from the organic molecules in food to ATP (Adenosine Tri-Phosphate). Glucose, CO2, and yeast (used as a catalyst in this experiment) are a few of the many vital components that contribute to cellular respiration. Sugar/ glucose is an important carbohydrate that can be made during photosynthesis from water and carbon dioxide, using energy from sunlight. Carbon dioxide is given off as a waste product when energy is released by the breaking down of glucose. This can be used by plant cells in the process of photosynthesis to form new carbohydrates. Yeast is a single-celled fungus that can break down sugars (glucose) to help produce carbon dioxide. Research Question How does sugar concentration affect yeast respiration rates? Hypothesis If I increase the sugar concentration then the yeast will produce more CO2 because the …show more content…
Next, weigh out 5 grams of sugar and 1 gram of yeast. Once in proportions, add it to test tube A. Thoroughly mix around the sugar, yeast, and tap water. You can do this by shaking the test tube gently. Make sure you do not turn it upside down. Then, cover the top of the test tube with a balloon and gently place it into a 400mL beaker filled up about half way (about 250mL) with water. Place the the beaker onto a hot plate that is on a low heat setting (about setting 3). Every 5 minutes for 20 minutes, measure the circumference of the balloon and record it in Data Table A. You can measure the circumference of the balloon by looping a piece of string around it then using a ruler to measure the string’s length. Record the data in the data
In this experiment the rate of cellular respiration was measured by the amount of CO2 in ppm per gram of substance produced by a given treatment group or the control over the course of ten minutes. CO2 levels were measured using a CO2 sensor. The sensor was given time to warm up then placed in a glass chamber with a sample from one of the treatment groups or a sample of control. CO2 levels in ppm were collected every four seconds for ten minutes by the sensor. The data was divided by the weight of the sample used to generate it, to give the respiration rate per gram of sample.
My hypothesis was confirmed because the yeast took a little amount of time to find the sugar since there was more yeast in the mixture than sugar. Once the yeast finds the sugar we saw a big jump in the CO2 production, meaning the yeast is consuming the sugar at a fast rate. But, once all of the available sugar is gone from the solution, we saw an abrupt stop. This abrupt stop was because there was no longer any sugar remaining for the yeast to consume so the production of the yeast by-product, CO2, halted and we no longer saw an increase in the air space. The standard deviation of the first part of the experiment compared to the standard deviation of the second experiment that we created are surprisingly accurate to what they should be.
+ ATP Although plants and animals have different methods of obtaining glucose, the cell respiration process occurs in both types of organisms. Many external factors in the environment may affect the organism's’ rate of respiration such as the temperature of the surrounding,
Figure4 - the chemical structure of sucrose. Figure5 - the chemical structure of lactose. Cellular respiration is when food molecules like glucose are oxidised to form carbon dioxide and water. Adenosine triphosphate is created by a catabolic pathway to be used by the cell.
Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2. Independent Variable pH 3. Controlled Variables temperature, amount of substrate (sucrose) present, sucrase + sucrose incubation time Effect of Temperature on Enzyme Activity 1.
Essay 79: Mitochondria Mitochondria are organelles found exclusively in eukaryotic cells, meaning protozoa, fungi, plants, and animals. The term 'mitochondrion' is derived from a Greek word meaning thread. This accurately describes their appearance in the light microscope, as barely visible thread like structures. Following the invention of the electron microscope, scientists learned that mitochondria have a complex structure that allows them to harness metabolic energy in a useful form. As an aside, prokaryotic cells (eubacteria, blue-green algae, and archebacteria) lack mitochondria but maintain a strong evolutionary kinship with them.
Aerobic cellular respiration and oxygenic photosynthesis are the main pathways of energy flowing in living things. Photosynthesis is known as a process that is used by plants, algae and bacteria get energy from sunlight into chemical energy. Aerobic cellular respiration makes ATP, finalizes e- acceptor is oxygen. Aerobic cellular respiration involves the products of photosynthesis, which are glucose and oxygen, while photosynthesis uses the products in aerobic cellular, which are carbon dioxide and water.
Title: How Ph Levels Affected the Fermentation of Beer Hypothesis: The beer will be left with more sugar deposit as the Ph levels increase because alpha/beta -amylase will no longer function. Predictions: Alcohol Percentage Analysis for the Control and the Experimental During this experiment, the pH level was increased, therefore Alpha-Amylase was favored. Due to the nature of Alpha-Amylase cutting randomly through a large carbohydrate molecule, it leaves bigger sugars in the flask, which cannot be digested by yeast. Due to this, less reactions should occur in the experimental, therefore leading to a lower percentage of alcohol production, compared to the control.
Glucose, which is a six-carbon sugar, is at that moment divided into two molecules of a three carbon sugar. The breaking down of glucose, takes place in the cell’s cytoplasm. Glucose and oxygen are produced from this breakage, and are supplied to cells by the bloodstream. Also produced by glycolysis are, 2 molecules of ATP, 2 high energy electron carrying molecules of NADH, and 2 molecules of pyruvic acid. Glycolysis happens with or without the presence of oxygen.
This process happens when it takes carbon dioxide from the atmosphere and ATP to produce
Background Information: Yeast fermentation is directly affected by the change in temperature, because the rate of chemical reactions is affected by temperature. If the yeast has been exposed to its optimum temperature (66.667 degrees Celsius) then it will give off the highest carbon dioxide production. As the temperature gets higher, the yeast will produce more carbon dioxide, until at some point carbon dioxide production will decrease, that is when the yeast cells have become denatured due to the increase in temperature. Chemical reactions
Dry ice is one of the coldest and most dangerous kinds of ice you’ll ever see. Dry ice can be made by blowing the fire extinguisher into a pillow case. Dry ice exerts carbon dioxide when it goes through sublimation. Sublimation is when the surface particles of a solid gain enough energy that they form gas. During Sublimation, particles of a solid.
Put the caps on the bottles and shake them until all the sugar is dissolved. Testing the Sugar Solutions All of the solutions should be the same temperature before testing Place the hydrometer in the 0% sugar bottle, record your reading in your notebook Repeat this step for the 5%, 10%, 15%, 20% and 25% sugar solutions. Rinse and dry the hydrometer between readings. Testing the Soda and Iced Tea Place the hydrometer in soda 1(coke).
An integral part of the study of biology is the study of cellular respiration and photosynthesis, which are both essential processes in living things. Without these pathways, living things would not survive as the intricate methods of converting energy into fuel are conceived in these two processes. The main purpose of both of these concepts are to form adenosine triphosphate (ATP), a molecule that contains the energy to fuel organisms. Though similar in goals, photosynthesis and cellular respiration have a lot of differences as well. The main difference between the two is that photosynthesis occurs solely in plants but cellular respiration occurs both in plants and other living organisms.
Then, tests are performed to determine if the products of aerobic and anaerobic respiration are present in the flasks. The citric acid cycle consists of a series of chemical reactions used by all aerobic organisms to release stored energy through the oxidation of acetyl-CoA derived from carbohydrates, fats, and proteins into carbon dioxide and chemical energy in the form of ATP (Biology). The tests detect the presence of carbon dioxide and ethanol. Carbon dioxide should be present irrespective of the type of respiration taking place, but ethanol is present only if fermentation has occurred. Another factor that can indicate whether fermentation occurred or cellular respiration occurred is the amount of glucose utilized during incubation.