Acetaminophen Within The Body Lab Report

The purpose of this experiment was to understand the pharmacokinetics of the drug acetaminophen within the body, specifically focusing on its partition coefficient, drug protein interaction and its bioavailability through various form of administration. The bioavailability of the drug was determined to be 100% for IV because the drug is injected directly into the systemic circulation in its active form and this is also visible on Figure 4, where the initial concentration of drug is much higher than in PO and IP. For PO and IP administration, the bioavailability was determined to be 72.6% and 39.1%, respectively. This makes sense because both of these type of administration involve the first-pass effect where a portion of the drug is metabolized by peripheral organs, especially the liver in this case, and therefore the amount of active drug reaching the circulation is less. PO administration, however has a much higher content reaching the circulation than IP, because the IP route involves passing through the whole gastrointestinal tract before being absorbed in the liver while the IP route injects the drug into the

Drugs bound to protein are not able to be excreted by the body because it doesn’t filter into the glomerular tubule and this is mainly because of its large size. In this lab the experimental value for binding of acetaminophen to serum albumin was calculated to be 21.93%. The binding of different drugs differ greatly in their ability to bind to serum proteins. One major problem in performing this experiment was that the plasma was contaminated with mint wax-dental floss that was used to tie the ends of the dialysis tube. This affected the absorbance reading of the plasma sample and therefore we had to use the data of another group whose plasma sample wasn’t as