Abstract: Many species must evolve to survive and adapt to their environment that is constantly changing. By looking closely at the protein structure of a species and understanding the DNA sequence, researchers can learn similarities and differences between species. This can give us a better understanding of how evolution works. This experiment will examine four samples and their actin and myosin amounts. Actin and myosin are used for muscle contracts and will therefor allow us to see how each sample adapts and uses these proteins to carry out their functions and how they relate to each other. Introduction: Proteins are essential to all species. They determine many things such as how an organism will look like, how their body can function, and performing tasks such as chemical reactions that are important to daily life. The building blocks for proteins are chains of amino acids which come from food sources and once digested, they come back together to form proteins that the body can use. Each chain of amino acid is specified by a gene with a unique DNA sequence. Mutations can occur within the DNA sequence …show more content…
Using a pipette, 250mL of Lamelli Buffer was added to each of the flip top tubes along with the sample. Each of the flip flop tubes was flick at least 15 times to insure the sample and buffer were mixed well together. After that, each of the samples were incubated, or left out sitting in room temperature for five minutes to extract and solubilized the proteins. The actin and myosin standard were then given out by the professor. This was for the fifth tube. After waiting for five minutes, the buffer from the flip top tubes was transferred to the screw top tubes and all five tubes were incubated in a 95° water bath to denature the proteins. The samples were stored in the freezer until the next class