Bright's Disease Lab Report Sample

Introduction

The purpose of this lab was to use chemical and physical tests to identify indicators of disease in synthetic urine samples. This lab tested samples for protein levels, glucose levels, and pH levels. In a normally functioning individual, proteins cannot pass through the glomerulus; therefore proteins should not be found in urine. However, in the nephrons of individuals with Bright’s Disease, the glomerulus no longer stops all proteins from entering the urine (Giuseppe et al., 2002, pp. 357–358). Bright’s Disease is characterized by a change in the permeability of the glomerulus, which allows proteins to pass through and since the nephron has no way of reabsorbing proteins they are passed into the urine (Giuseppe et al., 2002,

Then five millilitres of sample “A” were placed in the test tube labeled “A”. This was then repeated with the next three samples. Each sample was visually observed and the colour of each was recorded. Next 20 drops of Benedict’s solution were added to each test tube and the test tubes were lowered into a hot bath at a temperature of approximately 80 degrees Celsius. All colour changes were recorded. Next, the test tubes were carefully cleaned with soap and water. Then five millilitres of sample “A” was placed in the test tube labeled “A”. This was then repeated with the next three samples. Then a few drops of each sample were placed on glucose/ketone paper. Each piece of glucose/ketone paper (with the sample on top) was compared to the label on the glucose paper bottle. The percentage of glucose was recorded for each sample. Next, the test tubes were carefully cleaned with soap and water. Then five millilitres of sample “A” was placed in the test tube labeled “A”. This was then repeated with the next three samples. 20 drops of Biuret reagent were then added to each test tube. The colour of each test tube was recorded and if proteins were present that was recorded for each test tube. Finally, the pH was recorded for each sample using pH