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Coffee Araba And Daphnia Magna Experiment

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The main purpose of this experiment was to test the influence of Coffee (Coffee Arabica) and Kava-kava (Piper methysticum) on the heart rate of a Daphnia magna specimen. Based on the data collected it can be reasoned that Coffee (Coffee Arabica) and Kava-kava (Piper methysticum) have an influence on the heart rate of Daphnia magna. All three trials for both the Coffee test and the Kava-kava test displayed a trend. For the Coffee test, the after heart rate increased for all the trials performed. The average difference in beats/minute between the after heart rate and the baseline heart rate was 26.67. This positive average difference indicates that the alkaloid in coffee, caffeine, acted as a stimulant for the Daphnia magna. The Kava-kava test, …show more content…

The t-critical value was determined to be 2.23 by using the given chart in the lab manual. The calculations for the t-values of both tests yielded a score of 1.397 for the caffeine experiment and a score of 0 for the kava-kava experiment. If the t-values were greater than the critical value then the p-value would be below 0.05 thereby allowing us to reject the null hypothesis. However, in this experiment we fail to reject the null hypothesis (we accepted the null hypothesis), because both t-values are less than the t-critical value of 2.23. This implies that the class results obtained through this experiment verify that alkaloids in Coffee (Coffee Arabica) and Kava-kava (Piper methysticum) will have no influence on the heart rate of the Daphnia magna specimen. One article states, “…caffeine, surprisingly, has little or no effect on Daphnia heart rate” (ProQuest). Another scientist also published his research saying, “Caffeine could be used to emphasize the need for blind observers because it does not increase heart rate in Daphnia” (BioOne). These findings align with the conclusion we developed based off our statistical

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