Staphylococcus aureus
Introduction/ Background information
Staphylococcus aureus (S. aureus) is yellow-pigmented colonies and this is a reason for (aureus = golden) in its genus name. S. aureus is a spherical shape (Coccus), gram positive bacterium that usually present in pairs as seen in microscope.it has short chains, or bunched, grape-like clusters, non-motile, no spores and facultative anaerobic. Members of this species can survive in the aerobic or anaerobic conditions and they can adapt to any of situations. The best environment for them to grow is in dilute salt concentrations and low moisture, which partially introduces human's nasal secretions and skin's surface to be a suitable place for this microorganism to grow. This bacterial growth can lead to staphylococcal infections in skin, nose and throat of human
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Cytochrome Oxidase can work as a catalyst in aerobic respiration to reduce Cytochrome by using molecular oxygen to form water or hydrogen peroxide. Only aerobic organisms are able to produce cytochrome c in order to be able to use oxygen in their respiration process. In this test a small sample of bacteria is transferred in a filter paper containing few drops of oxidase reagent. Positive test result is indicated if the colour of filter paper changes from dark blue to purple colour and it changes below 10 seconds. If this colour change happens between 10 to 15 seconds, then the test result will be weak positive. Negative test result is indicated if no colour change is observed or colour changes after 15 second. Oxidase reagent is not stable once it is opened and used for few hours, but slowly will be oxidized by the oxygen. gram negative bacteria, can be either oxidise negative or oxidise positive. This test for Staphylococcus .aureus , and many other Staphylococcus like S. haemolyticus, S. eperdimis , S.simulans , will be oxidase