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Diluted Mixtures Lab Report

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First part of this experiment was to create and plan out a dilution series to figure out the concentration of Salmonella enterica in the original solution. Three plates were labeled 1,2, and 3. Plate 1 being the most diluted mixture and 3 being the least diluted mixture. These plates were examined to figure out the CFU. The dilution factors of the samples were 1:30,000 for plate 1, the second plate was 1:60,000, and the third and final plate was 1:120,000. 100uL of each sample was pipetted onto the center of their labeled agar plate. Sterile beads were then placed on top of the agar and agitated over the media to ensure equal spreading over the agar. The plates were allowed to sit at room temperature for 10min and then placed in the incubator

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