There was no indication of phenotypic differences between male and female mutant flies ,illustrating that the mutation was not sex linked because both mutant sexes possessed the same eye mutation. Male flies in both wild type and mutant flies consisted of the sex combs in the front of the legs, and dark coloration located at the end of the abdomen. Both classes of female flies possessed a tiger- striped abdomen and a tag-like tip at the end of the abdomen. After confirmation of our eye mutation we can assume that our mutation can be due to either genes rosy, garnet, brown, purple, and sepia because these are genes that affect the pigmentation color of the Drosophila melanogaster. Our mutation may be caused by the transportation of the enzyme xanthine dehydrogenase to the rosy gene which causes a deficiency in the pigmentation
Introduction: The objective of this lab was to study the trait of aldehyde oxidase (AO) in fruit flies. Aldehyde oxidase is responsible for catalyzing the oxidation of many aldehydes. The aldox gene controls the amount of AO activity in Drosophila melanogaster. In the first part of the lab, an enzyme spot test will be performed on two different vials of Drosophila to exhibit the AO activity of both vial 1A and 1B. A positive test for AO test will present a blue color, while a negative test will present no reaction.
The χ2 value was 0.012858, df was 3 and hence the P value was less than 0.001 which proves that this result is not a result of random probability and is in fact reliable. In Trial 3, twelve isopods (60%) made the choices of hiding behind the sucrose sponge. The χ2 value was 0.00350342, df was 3 and hence the P value was in between
We use Mendelian genetics to study the genetics of C. elegans. C. elegans have very similar genetics structure to humans. C elegans belongs to Phylum Nematode species which is very different from the earthworm. C. elegans is the first eukaryotic organism to have an entire genome sequence. It is very easy and simple to conduct an experiment on C. elegans that’s why the majority of laboratories use this organism.
At a level of significance of 0.05 the researcher is accepting a possible type I error 5 cases out of a 100 and 95 cases out of 100 a true null hypothesis (Loiselle et al., 2011). Step 3: Selection of one-tailed or two-tailed test The selection for a one-tailed or two-tailed test is not required for a Chi-Square for independence test (Salkind, 2013). Chi-Square test has both variables that are of a categorical level of measurement therefore, there is no direction (Salkind, 2013). Step 4:
Reciprocal cross is referred to females possessing the dominant allele that are mated with males possessing the recessive allele and females possessing the recessive allele that are mated with males possessing the dominant allele. We first had to isolate virgin fruit flies from the existing fly populations and then perform a reciprocal cross of the two populations. The reciprocal cross consisted of cross A- male's (+) wild type and females (wmf) mutant type. Cross B consisted of males (wmf) mutant type and females (+) wild-type.
Introduction C. elegans are 1mm transparent worms used in many experiments as a model organism, since they are creatures that are easy to use and take care of in a laboratory setting. These organisms are small in size, have a short three-day life cycle, and reproduce efficiently in the lab. The C. elegans only have two sexes, male and hermaphrodite and these sexes make the organism convenient for breeding and reproduction in the laboratory environment (Hope IA, 2005). One reason the C. elegans is so highly used in experiments is that it has the ability to detect some volatile chemicals with a process called chemotaxis; this organism is shown to chemotax towards certain compounds. Chemotaxis is the gradient formed by the movement of organisms towards an odorant, also known as a substance with a certain fragrance.
The purpose of this experiment was to conduct a long-term population genetics study by observing five generations of Drosophila melanogaster to determine the allele frequencies of eye color. Population genetics is the study of distribution and change in allele frequency in a population (). This makes population genetics important to the study of evolutionary biology as it examines adaptations, speciation, and the population structure of a population under study (). Two important processes of population genetics that will be examined in this experiment are natural selection and genetic drift, both that are important to evolution ().
In this egg drop project, a container was built to house one egg and was then dropped at various heights. The purpose of this lab was to find how impact time affected the force with which an object, the egg container, hits another surface as the momentum moves to zero. Our hypothesis states that if the impact time is stretched longer then the force exerted on the egg would decrease, making the egg less likely to break. The independent variables for the egg drop project was the height at each drop; the dependent variables were impact time and survival of the egg. The controls were mass, design of the container, and the egg.
A Demonstration of Chemotaxis Between Flies and Various Substances (Sugar vs Bacteria) Abstract: The purpose of this lab based on the Drosophila melanogaster (the common fruit fly) reactions. Since the fly has been studied and observed for many years, and known for its unique chemotactic attractions to different stimuli, it was an ideal organism for the study being conducted. In the experiment the purpose was to be able to figure out whether the flies would be more attracted to sugar or bacteria.
The purpose of this lab was to change pennies from copper to silver to gold, like alchemists have attempted to do in history. Through the data and observations gathered throughout this experiment, it can be concluded that the pennies were not changed into a different element. For example, the density of the penny from 2005; which was the penny that was experimented on to see whether or not it could turn into silver; was 4.62 g/cm3 before the experiment and 4.89 g/cm3 by the end of the experiment. If this copper penny really would have turned into silver, then the density of the penny would be 10.49 g/cm3; which is the density of silver; by the end of the experiment. The penny may have turned silver in color, but this was only because it was plated in the zinc that was added to the beaker of water in the experiment.
For eukaryotic cells, it has been believed that their is a succession of different CDK’s that are needed in different stages of the cell cycle. An example of this if in animal cells, there appears to be at least 3 CDK proteins and several different cyclins that operate just at G1, the first growth phase. The multiple CDKs and substrates are the driving factor behind cells going through the cell cycle and mitosis. Yeast are single-celled organisms that are eukaryotes since they have a nucleus where their genetic material is stored. Fission yeast have challenged this model since four CDK-cyclin complexes were replaced by one CDk-cyclin complex, which was composed of Cdc13 cyclin and Cdc2.
In this research paper I’ll be talking about the diet of a Venus Flytrap, what it can and can’t eat, and how it takes nutrients from things they eat. But first i feel like i need to explain what a Venus Flytrap is. The Venus Flytrap was discovered 1875 by Charles Dawson on , "Insectivorous Plants," that the Venus flytrap is "one of the most wonderful [plants] in the world. " There's no doubt that this opinion was formed after watching the jaws of this plant snap around an insect,capturing it for a meal.
The first one was done randomly by tossing a coin to determine their offspring's genotype. The shaded pairs were only breed with other shade pairs, and only produce male offsprings to next generation; non-shaded pairs were only breed with non- shaded pairs, and only produce female offsprings to next generation. When a coin was flipped, the outcome (H or T) would determine which allele would be inherited from mother or father. Each generation’s genotypes came from the last generation, for instance, the F1 genotypes came from founding population; and the F2 genotypes came from F1. There were 3 blocks and 8 generations in total, representing 3 couples and their
Since the 1700s, researchers have already been using statistical testing in search for answering many scientific or non-scientific questions (McLean & Ernest, 1998). From deciding a serious issue such as whether a patient needs a certain treatment, to a trivial matter such as deciding whether to buy a certain car or apartment, significant tests are used as a part of our decision’s making process. It provides us with conveniences and nurtures our quick decision-making process. It is so embroiled in our life that we seem to trust it so much without considering the significant errors it might have. There have been many ongoing debates by professionals from social sciences, researchers and doctors in deciding whether the abolishment of significance test is needed as it contains many controversies that are worthy to note (Lecoutre, Poitevineau & Lecoutre, 2005).
