ipl-logo

Enzyme Lab Report Essay

853 Words4 Pages

Abstract: In this experiment we are trying to figure out if different PH solution affects the activity of an enzyme, does it affect the enzyme? Or does it not affect the enzyme at all? We see that the PH 7 solution has the highest absorbing value, showing use the optimum PH while the other PH solutions are less. Showing us that PH does have an effect on enzymes but, also showing that PH 7 is the optimum PH, meaning that the reaction at the fastest rate than at any other pH level. Introduction: Does PH affect an enzyme’s activity? There are many things that that can affect an enzyme in different ways. Such as temperature affecting the reaction rate of an enzyme. Before all of that, we need to know what an enzyme is. Enzymes belong to a class of molecules known as proteins, this means …show more content…

We then added 1 ml of enzyme and 5 ml of distilled water. We need to make sure to cover the test tube with parafilm and made sure to mix the contents well. The spec 20 wavelengths had to be set to 540nm, we had to adjust it. We then used the spec 20 to set the absorbance read to infinity with the left knob and made sure to wipe the test tubes, any prints/oils could affect the experiment. Now using the right knob of the spec 20 we set the absorbance reading to 0, then removed the blank from the spec 20 and save it for later on in the experiment. We then obtained 4 test tubes labing them 1-4. Add 3 ml of distilled water in all 4 test tubes and add 3 ml of different PH solutions (5,7,9) in each test tube except for test tube numbered 4 and mix contents well. Then we added 1 ml of Catecholase in the first 3 test tubes contain PH and add 1 ml of Catechol in all test tubes, and be sure to put a parafilm on all test tubes. Measure the absorbance of all test tubes and be sure to use the blank test tubes after getting results from every absorbance reading (General Biology 1 Lab

Show More
Related
1407 Words | 6 Pages

LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.22.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3. Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2.

Read More
688 Words | 3 Pages

Objective Bio160 Lab 5: Enzyme Activity May 7, 2015 The objective of this experiment was to note the effect of temperature effects on enzyme rates of reaction. Enzymes are macromolecules that make up significant portions of living organisms. They are made up of repeating subunits of monomers that are referred to as polymers.

Read More
530 Words | 3 Pages

The Purpose To test and analyze the effect of pH and temperature on the reaction rate of the enzyme catalase. HYPOTHESIS The more base you add to the liver the reaction will be faster The more heated the liver is the longer it will take the reaction to take place than if the liver is cold or at room temperature, MATERIALS 8 test tubes Safety goggles Test tube rack Water Ice Bunsen burner Liver Hydrogen Peroxide (H2O2) Hydrochloric acid (HCL) Sodium Hydroxide (NaOH) Liver(cut into small pieces) Test tube holder 2 beakers Pipette Stirring rods Paper Towels Beaker stand Method Gather all the material and put on the safety goggles.

Read More
1166 Words | 5 Pages

3. (a) What effect does an extreme high temperature have on enzyme function? (b) What is the cause of this effect, in other words what exactly happens to the enzyme molecules due to extreme high temperature? Explain your answer in detail and supported by research. ANSWER:

Read More
720 Words | 3 Pages

How does it affect the enzyme Activity?: The value of pH is from 1 to 14 and pH can be neutral,alkali or acidic.pH 7 shows that it is netural and any number higher than pH7 is alkaline and lower than pH7 is acidic.pH are concentration of hydrogen ions that are found in solution. However, there is more concentration of hydrogen ions (protons) in acidic solutions and they are known as proton donors. If we look at the strucutre of the enzymes especially tertiary strucutre you can find bonds such as hydrogen bond and lots of ionic , which gives the shape to the active site and helps to keep them in place properly. These enzyme are made out of amino acid and within them you can find oppositely charged group that are attracted to each other , which

Read More
144 Words | 1 Pages

Enzymes Enzymes are proteins that work together in a cellular metabolic reaction, they catalyze the reaction. Even though, it is involved in a chemical reaction it returns back to its original state and prepares for its next chemical reaction. Enzymes come in many different shapes, the shape of enzyme provides a certain shape for the substrate to fit into, similar to a lock and key. When these two shapes connect to one and another a chemical reaction is then catalyzed by the enzyme and a reaction takes place in the substrate.

Read More
279 Words | 2 Pages

Enzymes are proteins that acts as catalysts, increasing the rate of chemical reactions. With containing many different enzymes, enzymes prove to be viable molecules, as they can ultimately determine the many possible chemical reactions that take place within the cell.1With an enzyme able bind to the substrate through specificity, the substrate correctly orients itself binding to the active site. Once positioned, the enzyme can lower the activation energy of the reaction causing the chemical reaction to occur at a rapid pace. The efficiency of an enzyme can vary depending on the environment. Some factors may include temperature, pH, and differences in enzyme substrate concentration.

Read More
730 Words | 3 Pages

Enzyme speed chemical reactions by lowering the activation energy, activation energy is the energy require for chemical reactions to take place. Enzyme is the ES complexes that lower the activation energy. There are two ways to form product in enzyme, one is two substrates to form one product in one active site or one substrate break down into two products. If two substrate molecules need to be joined to form one product, two substrates need to attached to the enzyme holds them close together, reducing any repulsion between the substrate molecules so they can bond more easily to form one product. If the enzyme is catalysing a breakdown reaction, it means one substrate breakdown into two products, one substrate molecule fitting into the active

Read More
1014 Words | 5 Pages

4.Effect of pH Each enzyme has an optimum pH which it attains its maximal activity

Read More
159 Words | 1 Pages

Enzymes are globular protein molecules that act as catalysts by lowering the activation energy. Like all globular proteins, enzyme molecules are coiled into a three-dimensional shape with hydrophilic R groups on the outside of the molecule making them soluble in water. Enzymes control all metabolic and anabolic reactions, both intracellularly and extracellularly. A substrate is a molecule on which the enzyme acts. An enzyme combines with a substrate to form a short-lived enzyme-substrate complex.

Read More
1247 Words | 5 Pages

Through the oxidation of those phenols, a chemical reaction that causes a loss in electrons is present which usually results in a red or brown color. Due to the varying levels of reaction possibilities that catechol can undergo when in contact with DNA makes it toxic to humans (Eggen RI, Schweigert, Zehnder AJ). In order to measure enzyme activity through the different environmental parameters, the experiment will need utilization of a spectrophotometer to record the change in absorbance and compare how different temperatures either support or inhibit enzymes. In this, ethylenediaminetetraacetic acid (EDTA) was used to act as a chelator which aids in the removal of ions, this provided favorable environments for enzymes to speed up the reaction process. On the other hand, phenylthiourea (PTU) was used as an inhibitor that only worked if catechol oxidase had copper as a

Read More
311 Words | 2 Pages

The countless chemical reactions that take place within our cells do not simply occur haphazardly, but rather occur through a highly controlled system of protein catalysts called enzymes. Like any other catalysts, enzymes function to speed up the rates of chemical reactions by lowering the activation energy or the energy needed to start the chemical reaction. When no enzymes are present, the catalyzed chemical reaction does not occur at a desirable rate. Therefore, without these essential and crucial enzymes, life on Earth would cease to exist because the speed of all chemical reactions would be too inefficient for a living being to function. Enzymes are usually grouped together based on their specialization and function.

Read More
452 Words | 2 Pages

Enzymes are biological molecules, typically proteins, that speed up the rate of reactions by lowering the activation energy needed for the reaction. Different enzymes speed up different reactions. The substrates bond onto the active site of the enzyme, where the enzyme react with the substrate to release products. The active site of the Enzyme affects what substrate could react with the Enzyme because the shape of the substrate has to be able to fit inside the enzyme. Without these enzymes, the reactions would happen much more slowly.

Read More
1167 Words | 5 Pages

5 water bath were set up each to10 °C. (5 were used do the experiment faster) 5 cm3 of starch solution were added into the 5 test tubes that were labeled test tubes. Then 5 cm3 of amylase enzyme was added into the other 5 test tubes that were labeled. Put one of the starch solution test tube (preferably the one labeled 1) and one of the test tube containing amylase into the water bath (10 °C).

Read More
1046 Words | 5 Pages

ABSTRACT: The purpose of the experiments for week 5 and week 6 support each other in the further understanding of enzyme reactions. During week 5, the effects of a substrate and enzyme concentration on enzyme reaction rate was observed. Week 6, the effects of temperature and inhibitor on a reaction rate were monitored. For testing the effects of concentrations, we needed to use the table that was used in week 3, Cells.

Read More

More about Enzyme Lab Report Essay

Open Document