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Escherichia Colo Lab Report

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Transformation in bacteria usually takes place when a bacterial cell accepts strange DNA and integrates to its own DNA. The transformation normally takes place within plasmids, which are tiny circular DNA molecules that have been separate from its own chromosome. The copies of the same plasmid range from 10 to 200 copies within a cell. These copies of plasmids may multiply when the chromosome replicate or multiply independently. One plasmid has a range of 1,000 to 200,000 base pairs. R plasmids are responsible for carrying the gene for resistance to antibiotics e.g. ampicillin, which are normally used in the lab. The normal function of a plasmid is to transport genetic information essential to the survival of the bacteria. (Barnnet, 1995). The plasmid can work as vectors for introducing strange DNA. Restriction enzymes are normally used cut foreign DNA and placed it into the plasmid vectors. This lab used Escherichia coli (E. coli) bacteria (Kok , 19840). This is because Escherichia coli can be simply grown in Luria broth or on agar, and also has a comparatively small genome of five million base pairs. …show more content…

Strains of E. coil are important in the digestive tract, but others may cause problems in urinary and intestinal tracts. Certain types of E. coli strains show resistance to bacteria which kills antibiotics. The resistance is because of the plasmids. Resistance plasmids are broadly studied and bestow resistance to factors which may hinder growth of the organism. Resistance plasmids signs for proteins which will inactivate the antibiotic affect their reception into the bacteria (Weinreich, 2006). We deal with two different strains of E. coli in this lab i.e. Standard E. coli which lacks resistance plasmid and strain containing a resistance plasmid which has genes that are protecting it from

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Transformation was successful in the plates where the bacteria consumed the pGLO plasmid. In the first plate that the bacterium was plated on it included the LB broth and of ampicillin antibiotic (amp), 2 colonies were present. The second plate of bacteria was grown with the presence of LB broth, ampicillin, arabinose sugar (ara), and 22 colonies were observed. But a green fluorescent glow of the colonies was only present in plate 2. Plates 3 and 4 were the control plates.

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Where natural selection occurs when a portion of the population dies off due to the ampicillin, while the rest of the population manage to survive when carrying a resistant gene. As the continuation of survival, the E. coli will develop an immunity against the ampicillin. Making it difficult to eliminate, while it multiplies for a new population of resistant E. coli. When the bacterium become resistant against the antibiotic, patients with the infection will take a longer time to recover to fight off the

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