Examples Of Pglo Transformation Lab Report

In this lab, the goal was to transform bacteria with genes that included fluorescence as well as antibiotic resistance that were taken from a jellyfish. Transformation is transferring a gene from one organism to another. Certain precautions had to be made before doing this lab since every step had to be done very quickly to prevent too much contamination. The first step in starting the transformation is to add the transformation solution into the +pGLO and -pGLO test tubes. After this is done, you put both tubes in ice and then put bacteria in both tubes. Then, put the pGLO plasmid in the +pGLO test tube but not the -pGLO. Place both of the tubes back in the ice. While the tubes are in the ice, label 4 agar plates: +pGLO LB/amp, +pGLO LB/amp/ara,

In more detail, transformation is the process of a cell taking in new, foreign DNA into its nucleus. This causes a new gene to be created that is not originally part of the cell. This often results in a new protein being creating in the cell. In this specific case, the protein that makes the Aequorea victoria jellyfish fluorescent, is transferred into the bacteria. The process of transformation can be used in a variety of fields including agriculture, bioremediation, and medicine. Though this is usually beneficial, transformation can lead to bacteria developing the ability to be resistant to antibiotics by having a mutant bacteria cell that has resistance, spreading that resistance to every bacterial cell it can find. Transforming single cell organisms is also much easier than transforming multi-cellular organisms because in order to affect a whole unicellular organism, you must transform every cell whereas if you want to transform a unicellular organism, you only have to transform one cell. Also, the reason why only one plate successfully glowed is because that plate was the only one where the pGLO plasmid and arabinose were present at the same

Without this antibiotic, every single plate would have lots of bacteria on it, only the one successful plate(+pGLO LB/amp/ara) would have a few glowing spots surrounded by lots of untransformed bacteria. The reason that this place was successful is because the transformed bacteria were able to resist the antibiotics, and also were also able to use the gene to make fluorescent proteins because the sugar arabinose was