7.1 General Discussion The study of Glaciozyma antarctica PI12 concerning its ability to adapt under a changing environment, especially during temperature shifts, has shown that the unicellular yeast has the ability to thrive and alters its gene expression patterns to encounter with the stress caused by the temperature shifts. Cold adapted yeast Glaciozyma antarctica PI12 in this study, referred to a yeast that was isolated from Antarctica, which able to grow at low temperatures. An in-depth overview of its characteristics and the various gene expression patterns under the thermal changes is reported in the previous chapters. In brief, a set of de novo transcriptome data was generated in order to identify all of the functional transcripts …show more content…
The doubling time refers to the time a cell requires to double the amount of its original population numbers. However, it is arduous to comprehend the factors underlie within the cell, peculiarly in the determination of the cell proliferation rate and doubling time of a cell. It is inconceivable to have a similar proliferation rate and doubling time across the organisms. Proliferation rate and doubling time of a cell often affected by the complexity of a cell. For instant, when comparing two unicellular organisms, such as yeast and bacteria, S. cerevisiae shown to have a much slower proliferation rate (100 minutes per generation) as compared to E. coli, which proliferation rate is 15 - 20 min per generation (Herskowitz, 1988). In addition, the proliferation rate and doubling time would increase proportionally as the size of an organism increases. Nevertheless, there are many other factors that would influence the proliferation rate of a cell, including the chemical composition of a media, environment, water sources and water availability, carbon sources, nutrient sufficiency, pH and et