The aim in this experiment is to estimate the Glomerular Filtration Rate (GFR) from the renal clearance of creatinine. In doing so, a graph on absorbance versus concentration of creatinine has been made to measure the GFR. On the y-axis is the absorbance meanwhile x-axis is the concentration of creatinine. The standard curve and best fit line is drawn to obtain a linear function between absorbance and the concentration of creatinine. The result shows that the absorbance value increases linearly to the concentration of creatinine. This means that the absorbance follows the concentration or amount of creatinine. For example, 0 mg/ml of creatinine have 0 absorbance, 0.005 mg/ml have 0.011 absorbance, 0.01 mg/ml have 0.084 absorbance and 0.02 mg/ml …show more content…
These consist of 3 ml urine (1:50 dilution), 3 ml urine (0.5:250 dilution) 3 ml non-diluted urine and 3 ml plasma. The calculation being used is to determine the renal clearance (ml/min) from which it is equal to GFR. The average of U, concentration of creatinine in urine (ml/mg) is 3.618 ml/mg). This value is obtained using the spectrophotometric method in obtaining the absorbance or concentration of creatinine from different concentration of urine. V is volume of urine collected per minute from the sheep that is 3.1 ml/min. Meanwhile, P, the concentration of creatinine in plasma is obtained from the blood taken from the jugular vein within the half period during urine collection. Why this is done, so that the concentration of creatinine in blood can be assume nearing to the concentration of creatinine in the urine. P value is 0.647 mg/ml. Based on the result and calculation obtained, the GFR is determined that is 0.722 ml/min/kg with accordingly to its bodyweight, 24 kg after …show more content…
In the kidney urinary system, the organ involve in regulating the excretion of wastes is the kidneys, ureters, urinary bladder and urethra. In excretion of wastes, the blood is filtrated where some of the substance is reabsorbed back into the body through reabsorption and some are maintain in the filtrate; usually toxic and wastes and removed from the body. How is the filtration occur is with the help from nephrons; the functional unit of the kidney. The main function of nephron is to filters the blood and involve in formation of urine. At the same time, arterioles that present in the kidney helps in draining the blood into the glomerulus, the bundle of capillaries that enclosed with a capsule whereby the blood’s plasma is forced into the capsule from the capillaries. Blood cells and little amount of plasma remain flows in the