Following are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Beta glucouronidase test is used for the identification of Escherichia coli. An enzyme is produced by E.coli which is beta D glucouronidase. Beta d glucouronidase in turn hydrolyzes beta d glucopyranosid uronic derivatives to aglycons and D glucuronic acid. Bile solubility test is used in laboratory for differentiation of alpha hemolytic Streptococci from Streptococcus pneumoniae.
In catalase test it acts as a catalyst for the breakdown of hydrogen peroxide to oxygen and water. Catalase production test is done for an organism by bringing it into association with hydrogen peroxide. If an organism is
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In the test for a reducing sugar, if it changes to a red orange color is it known as a precipitate as it comes out of the solution and forms solid particles dispersed around the water. The test of non-reducing sugar is striving as a result of if there is any sucrose presents it is broken down into those monosaccharides, which can be proved for using the common reducing sugar test. A positive result indicates that non-reducing sugars are present on the original sample. Sucrose fermentation it involves inoculating of sucrose broth with inoculating loop. Usually done for the differentiation of Enterobacteriaceae species. A positive result gives yellow color after the addition of indicator. A negative result shows no change in color or has red color. In glucose fermentation inoculation of broth is done by using inoculating loop. This test distinguishes Enterobacteriaceae family. This test is done for testing bacteria ability to ferment glucose and gas production. Yellow color shows the positive result for an acid. For gas positive result bubbles are seen in the test tube. A negative result shows either reddish color or no color change and also no gas