Introduction: This experiment was performed to identify the unknown Species given, using selective and differential media. Materials and Methods: To begin this experiment, The Gram stain procedure will help identify a Gram-positive or Gram-negative bacteria. It begins with grabbing a clean glass slide and placing it on the glass staining rack. Flaming the inoculating loop sterile, use the DI water to create a bubble of DI water in the loop and placing it the center of the glass slide. Then flaming the unknown bacteria tube over the Bunsen burner as well as once again the inoculating loop, retrieve a good size amount of bacteria, making sure to close the tube after. Mixing the bacteria with the DI water already on the slide. Constantly flaming …show more content…
Once the slide is dry, cover the smear with crystal violet stain and let sit for 1 minute. After the minute has passed grab the slide with a wooden clothes-pin, tilling the slide at an angle and rising it off with DI water until it runs clear. Dry the slide off by gentile blotting it in bibulous paper. Place it back on the staining rack one the slide it completely dry. Cover the smear again but this time using Iodine stain and letting sit for 1 minute. Grabbing the slide with a wooden clothes-pin and tilling it at an angle, rinse the slide with DI water. Blotting it dry with the bibulous paper. Grabbing the slide with the clothes-pin, tilted at the angle let the ethanol to run down the slide until it runs clean. Then immediately rise the slide with DI water until run clean again. Once more blot the slide with the bibulous paper. Place it on the staining rack and add safranin and wait 1 minute. After take the slide with the clothes-pin and rise it with the DI water, placing it in the bibulous paper to blot it dry. Now done doing all these procedures of the gram stain, the slide can be observed under an oil immersion microscope. Making sure to start on the scanning and working up until the oil immersion, always keeping