Introduction Bacteria can be found in bodies of water where it takes many different shapes and sizes. Bacteria can be found in clumps associated with a specific particulate matter. The enumeration of bacteria cannot be counted individual but by the amount of clumps it forms. When found in clumps, it is identified by a coliform in every colony. [4] Total coliforms are a type of bacteria formed from different types of sources. Fecal coliform is produced when the bacteria is fermented and incubated at a specific temperature. In this experiment, my group and I were given a sample of natural drinking water to see if there was any fecal bacteria present. The main goal of this experiment was to find out if the sample we tested passed the standard safe drinking water. When experimenting the sterile technique is the safest way to not cross contaminate and bring other organism to the sample. It is important to use the aseptic technique and sterile all equipment used. The sterile technique is used to transfer one medium to another. The Bunsen burner is used to sterilize every equipment before use. Flaming the equipment before using them …show more content…
We started by placing a grid side membrane filtration in the vacuum pressure to start the process. When grabbing the membrane we flamed the tweezers using the sterile technique. We used 25ml of sample water measured in a beaker to pour in vacuum. The beaker was flamed before and then the sample was poured. After the sample, we used sterile water to rinse the membrane. When the vacuum removed the excess sample, with sterile tweezers we placed membrane in media to be incubated. After the membrane agar was incubating for twenty-four hours we were able to see coliforms formed. There were twenty three colonies that formed that were bright enough to see. Clean water should have zero coliforms formed and so this was another positive site of fecal