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Lab # 1: Examining And Verifying Various Macromolecules

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Lab #1: Examining and Verifying Various Macromolecules’ Reactions for Categorization of the Tested Solutions Jae-eun Park 20608251 Lab Partners: Jasmine Harding-Bake, Karolane Blais TAs: Lilia Shabon, Ryan Rashidi BIOL 130L Section 015 Experiment performed on: September 21 2015 Monday 2:30PM – 5:20PM B2 151 INTRODUCTION Various macromolecules share similar characteristics due to their shared functional groups, and in this lab, this was examined and categorized through three tests, namely the iodine test, Benedict’s test, and the biuret test. Iodine test will show the presence of starch and glycogen of the tested material by the color change to dark blue and earthy red respectively from its normal lightly yellow color. This …show more content…

(Wood, 2011.) From the initial blue color, if the solution’s colors change to brown, red, yellow or green, it proves that the aldehyde functional group, acting as the reducing agent, is present and thus the solution is a reducing sugar, because when the free aldehyde group of reducing sugars are heated up and reacted with CuSO4, the electrons will emerge from the aldehyde group to form Cu2O which would have a brown tint. (Simoni et al., …show more content…

Biuret is used to determine the presence of peptides and proteins of the solutions as the reduction of copper (II) ions to copper (I) would lead for the molecules to rearrange, resulting in the change of colors from blue to purple as the wavelengths of light absorbed would differ. (Wood, 2011.) It was hypothesized that only #9 protein would only be affected by the biuret test as it has peptide bonds, and it was confirmed by the experiments as a positive result was given. The unknown solution #281 did not change its color to purple and remained blue, thus it is concluded that the solution did not have peptide bonds in it. (Ellman,

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