BB1802 1618883 BB1802 Microscopy coursework- Introduction- Figure 1: shows the different parts of a light microscope. Light microscopes are microscopes used to examine objects using light. Light microscopy is one of the least invasive techniques used to access information from various biological scales in living cells. The combination of molecular biology and imaging provides a bottom-up tool for direct insight into how molecular processes work on a cellular scale (Antony et al.,2013). The figure 1 above shows the different parts of the light microscope. The light source provides …show more content…
Gram-negative bacteria are surrounded by a thin peptidoglycan cell wall, which itself is surrounded by an outer membrane containing lipopolysaccharide. Gram-positive bacteria lack an outer membrane but are surrounded by layers of peptidoglycan many times thicker than is found in the Gram-negatives (J.Silhavy,Kahne and Walker,2010). Which is why after treatment with a decolorizer the lipid layer from the Gram-negative cells is dissolved whereas, in contrast, the solvent dehydrates the thicker Gram-positive cell walls closing the pores and as a result diffusion cannot happen so the bacteria remain stained (Personal.psu.edu,2017) as seen in image 4 the rod-shaped bacteria have a purple-violet …show more content…
and Kozak, K. (2013). Light microscopy applications in systems biology: opportunities and challenges. Cell Communication and Signaling, 11(1), p.24. J.Silhavy, T., Kahne, D. and Walker, S. (2010). The Bacterial Cell Envelope. Cold Spring Harbor Perspectives in Biology, [online] 2(5). Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2857177/ [Accessed 1 May 2017]. Personal.psu.edu. (2017). Lab3gramstain. [online] Available at: http://www.personal.psu.edu/faculty/k/h/khb4/enve301/301labs/lab3gramstain.html [Accessed 28 April 2017]. Wwwmgs.bionet.nsc.ru. (2017). LARGE INTESTINE. [online] Available at: http://wwwmgs.bionet.nsc.ru/mgs/gnw/trrd/thesaurus/Di/large.html [Accessed 1 May 2017]. Madigan, M., Martinko, J., Bender, K., Buckley, D. and Stahl, D. (2015). Brock Biology of Microorganisms. 14th ed. Boston: Pearson, p.52. Madigan, M., Martinko, J., Bender, K., Buckley, D. and Stahl, D. (2015). Brock Biology of Microorganisms. 14th ed. Boston: Pearson, p.51. Microbiologyonlineblog.blogspot.co.uk. (2017). Microbiology Online Focus On: Parts and Functions of a Light Microscope (Part II). [online] Available at: http://microbiologyonlineblog.blogspot.co.uk/2009/11/microbiology-online-focus-on-parts-and.html [Accessed 25th April