5. Results and Discussions
5.1 Methyl erucate synthesis
5.1.1 Chemical analysis of erucic acid
Physicochemical properties of erucic acid used for the synthesis of sucrose erucate were analyzed. The results of analysis are depicted in Table 5.1
Table 5.1 Analysis of erucic acid
Acid value 166.03 ± 0.9 mg KOH/gm
Iodine value 78.04 ± 1.2 g I2/100gm
Saponification value 168.05 ± 0.8 mg KOH/gm
5.1.2 Analysis of methyl erucate
After synthesis and purification of synthesized methyl erucate general characteristics of product were tested. The results obtained are shown in Table 5.2 and Figure 5.1 Figure 5.1 Color of methyl erucate
Table 5.2 General characteristics of methyl erucate
Color Light yellow
Odour
Consistency
The results of chemical analysis
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Figure 5.2 TLC of methyl erucate [Solvent: chloroform: hexane (1:1)]
Rf value = (Distance travelled by solute )/(Distance travelled by solvent front)
=
5.1.5 Spectroscopy analysis using Fourier Transform Infrared Spectroscopy (FTIR)
The infrared spectra of the purified methyl erucate showed the characteristics band at 1739 cm-1 which attributed to carbonyl ester band (Figure 5.3). The other absorptions attributed to the molecule are shown in Table 5.4. Also the IR absorption peak can be compared as shown in Figure 5.3 and Figure 5.4.
Table 5.4 Spectroscopic identification of erucic acid functional groups in FTIR
Absorption frequency range(cm-1) Absorption peak (cm-1) Type of vibration
3020-3100 2920 =C-H stretch of alkene
2850-3000 2852 C-H stretch of alkane
1705-1720 1710 C=O stretch of acid
1350-1480 1452 -CH bend of alkane
1210-1320 1284 C-O stretch of acid
675-1000 931 =C-H bend of alkene
720-725 721 C-H rock of alkane
Figure 5.3 FTIR spectra of erucic