ipl-logo

Digest Starch Lab Report

1148 Words5 Pages

Results

Concentration of NaCl verses the time taken to digest starch
Concentration of NaCl (mol)
Time taken for amylase to digest starch (s)
Control (0.0)
110
0.1
116.67
1.0
300
3.0
100
6.0
100

Discussion

The data partially supports the hypothesis of chloride ions increasing the efficiency of amylase. In the 3.0 mol and 6.0 mol, the hypothesis was supported by the average time of digestion taking 100 seconds for both, however the 0.1 mol and 1.0 mol took longer to digest the starch with the 1.0 mol being an anomaly [see figure 1]. The results obtained showed that the higher the concentration of NaCl the faster the the starch will be digested. The results collected were not within a reasonable margin of error to have decisive …show more content…

This experiment showed that as the concentration of NaCl increases so to does the speed at which the digestion took place. However, the 1 mol test showed to be an anomaly with all tests conducted showing that there was no starch digested. The errors present in this test meant that the results were invalid but showed to an extent how NaCl affects amylase in the digestion of starch.

References

Aghajari, N., Feller, G., Gerday, C. and Haser, R. (2018). Structural basis of α-amylase activation by chloride.
Bbc.co.uk. (2018). BBC - GCSE Bitesize: What are enzymes?. [online] Available at: http://www.bbc.co.uk/schools/gcsebitesize/science/add_aqa_pre_2011/enzymes/enzymes1.shtml [Accessed 7 Mar. 2018].
Biology.arizona.edu. (2018). Energy, Enzymes, and Catalysis Problem Set. [online] Available at: http://www.biology.arizona.edu/biochemistry/problem_sets/energy_enzymes_catalysis/01t.html [Accessed 7 Mar. 2018].
Cooper, G. (2018). The Central Role of Enzymes as Biological Catalysts. [online] Ncbi.nlm.nih.gov. Available at: https://www.ncbi.nlm.nih.gov/books/NBK9921/ [Accessed 7 Mar. …show more content…

(2018). Enzyme regulation. [online] Available at: https://www.khanacademy.org/science/biology/energy-and-enzymes/enzyme-regulation/a/enzyme-regulation [Accessed 25 Feb. 2018].
Levitzki, A. and Steer, M. (2018). The Allosteric Activation of Mammalian alpha-Amylase by Chloride.
Lopina, O. (2018). Cite a Website - Cite This For Me. [online] Cdn.intechopen.com. Available at: https://cdn.intechopen.com/pdfs-wm/54390.pdf [Accessed 25 Feb. 2018].
Rsc.org. (2018). Chemistry for Biologists: Enzymes. [online] Available at: http://www.rsc.org/Education/Teachers/Resources/cfb/enzymes.htm [Accessed 25 Feb. 2018].
Wang, N. (2018). Starch Hydrolysis by Amylase. [online] Eng.umd.edu. Available at: https://eng.umd.edu/~nsw/ench485/lab5.htm [Accessed 7 Mar. 2018].
Worthington-biochem.com. (2018). Effects of pH (Introduction to Enzymes). [online] Available at: http://www.worthington-biochem.com/introbiochem/effectsph.html [Accessed 7 Mar. 2018].
Secondary Data Graph
Feller, G., Bussy, O., Houssier, C. and Gerday, C. (2018). Structural and Functional Aspects of Chloride Binding to Alteromonas haloplanctis α-Amylase. [online] Jbc.org. Available at: http://www.jbc.org/content/271/39/23836/F1.expansion.html [Accessed 8 Mar.

Show More
Related
2136 Words | 9 Pages

Although it was expected for water to be the optimal pH, it was also assumed that more drastic activity would happen with the other pH’s. For example, it was thought that it would still have some noticeable increase; however, when looking at the data and the graph, the numbers oscillate with no noticeable positive or negative trend. Tables 1 and 2 show that the absorbance rate in comparison to the absorbance rate in Table 3 are significantly smaller. Furthermore, after calculating the processed data for reaction rates and looking at the graph, pH 7 water had the highest rate. This experiment gives a good insight for future references about enzymes and the effect of environmental factors and its functions.

Read More
1117 Words | 5 Pages

The effect of pH on the speed of enzyme interaction with substrate chemicals Hypothesis: About pH: If the pH level is less than 5, then the speed of the enzyme reaction will be slower. About temperature: If the temperature stays the same, then the speed of the enzyme reaction will not be completely affected. Background information: The function of enzymes is to speed up the biochemical reaction by lowering the activation energy, they do this by colliding with the substrate.

Read More
1419 Words | 6 Pages

LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.26.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3.

Read More
529 Words | 3 Pages

In the control, beta-amylase was present unlike the experiment, which resulted in less molecules lingering. Discussion: 1. a. My results matched my prediction regarding alcohol percentage by weight.

Read More
1764 Words | 8 Pages

Enzymes speed up chemical reactions enabling more products to be formed within a shorter span of time. Enzymes are fragile and easily disrupted by heat or other mild treatment. Studying the effect of temperature and substrate concentration on enzyme concentration allows better understanding of optimum conditions which enzymes can function. An example of an enzyme catalyzed reaction is enzymatic hydrolysis of an artificial substrate, o-Nitrophenylgalactoside (ONPG) used in place of lactose. Upon hydrolysis by B-galactosidase, a yellow colored compound o-Nitrophenol (ONP) is formed.

Read More
740 Words | 3 Pages

Title: Enzymes Abstract: Enzymes can catalyze chemical reactions by speeding up the chemicals activation energy. Temperature and pH are just two of the factors that affects enzymes and their involvement with chemicals and the way they function. Throughout this experiment, we conducted a study on peroxidase, which is an enzyme. The following information consist of the recordings of when it was exposed to four different pH levels to come up with an optimum pH and IRV at the end. Introduction: Enzymes are proteins that are used in reactions in living organisms.

Read More
1940 Words | 8 Pages

Introduction Chemical reactions are seen in many instances, including those in which one substance is being converted to another. Natural chemical reactions will occur without intervention, however they occur slowly. Enzymes become important in these situations. Enzymes are proteins that act in cells to ensure reactions occur at appropriate speeds. In other words, they act as catalysts.

Read More
901 Words | 4 Pages

Introduction: Enzymes are needed for survival in any living system and they control cellular reactions. Enzymes speed up chemical reactions by lowering the energy needed for molecules to begin reacting with each other. They do this by forming an enzyme-substrate complex that reduces energy that is required for a specific reaction to occur. Enzymes determine their functions by their shape and structure. Enzymes are made of amino acids, it 's made of anywhere from a hundred to a million amino acids, each they are bonded to other chemical bonds.

Read More
485 Words | 2 Pages

3. Look at your graph for Part B, how does temperature affect enzyme activity? The colder the temperature the greater the reaction. 4. Look at your graph for Part C, how does pH affect the enzyme activity?

Read More
750 Words | 3 Pages

Introduction: Enzymes are biological catalysts that increase the rate of a reaction without being chemically changed. Enzymes are globular proteins that contain an active site. A specific substrate binds to the active site of the enzyme chemically and structurally (4). Enzymes also increase the rate of a reaction by decreasing the activation energy for that reaction which is the minimum energy required for the reaction to take place (3). Multiple factors affect the activity of an enzyme (1).

Read More
2575 Words | 11 Pages

Introduction 1.1 Aim: To determine the kinetic parameters, Vmax and Km, of the alkaline phosphatase enzyme through the determination of the optimum pH and temperature. 1.2 Theory and Principles (General Background): Enzymes are highly specific protein catalysts that are utilised in chemical reactions in biological systems.1 Enzymes, being catalysts, decrease the activation energy required to convert substrates to products. They do this by attaching to the substrate to form an intermediate; the substrate binds to the active site of the enzyme. Then, another or the same enzyme reacts with the intermediate to form the final product.2 The rate of enzyme-catalysed reactions is influenced by different environmental conditions, such as: concentration

Read More
1167 Words | 5 Pages

Research question What is the effect of temperature Amylase activity? Word count-1453 Background research Enzymes are biological catalysts that speed up a chemical reactions. They do this by decreasing the activation energy(the energy needed to start the reaction) of a chemical reaction. The enzyme present in our saliva is called Amylase. Amylase increases the rate of reaction by decreasing the activation energy needed to hydrolyse the starch molecules.

Read More
1046 Words | 5 Pages

ABSTRACT: The purpose of the experiments for week 5 and week 6 support each other in the further understanding of enzyme reactions. During week 5, the effects of a substrate and enzyme concentration on enzyme reaction rate was observed. Week 6, the effects of temperature and inhibitor on a reaction rate were monitored. For testing the effects of concentrations, we needed to use the table that was used in week 3, Cells.

Read More
916 Words | 4 Pages

In this experiment , we can prove that the temperature, pH and salt are the factors that will affect the structure and function of the enzyme as it is a kind of protein . Therefore, there may be an influence on the activity of enzyme which substrates cannot be binded on the active site if the amylase in too high or low ph and temperature and excess salt environment . On the other hand optimum ph and temperature and suitable salt concentration may favour the amylase activity . Reference : 1.2016, May 08). Effects of pH on Amylase Activity.

Read More
1437 Words | 6 Pages

INTRODUCTION: Arginase is an enzyme- enzymes are biological catalyst which drives a reaction at the speed of life. Arginase is a hydrolase, hydrolases catalyze hydrolysis reactions, this is determined via the E.C number (Nelson and Cox 2008). Arginase has the EC number is 3.5.3.1 (Schomburg 2015). The enzyme ‘commission number’ is the arithmetical classification that is used for enzymes which indicates the chemical reaction they catalyze.

Read More

More about Digest Starch Lab Report

Related Topics

Open Document