The experiment is work well. According to the result that I collected in lab, the bacteria in the LB negative plate is growth because the LB broth help bacteria grow. In the LB/AMP negative plate, the bacteria are not growth. The reason why they are not grow because in the LB broth, we put AMP in there; so the bacteria’s cell are sensitive with AMP. Furthermore, in the LB/AMP positive plate, the bacteria are grow even though the plate contain AMP. However, because bacteria E.coli accepts the pGlo plasmid and the plasmid can encode the gene for resistance to antibiotic, they are survived and grow in single colonies (Nature new, 2017). Finally, in the LB/AMP/Arabinose positive, the bacteria grow with green under the UV light. Like LB/AMP positive plate, the bacteria are …show more content…
Scientists use transformation as a tool to work on ways to treat human diseases and conditions. For example, the insulin that diabetes human use to treat themselves are made by this transformation. It means that this experiment is very helpful for scientist when they found how they can make insulin to treat patients. Likewise GFP gene in the transformation, Insulin is the protein in a human gene; it balances the glucose level in the body. As same as the technique, the scientist will cut the human insulin gene from some human DNA. Then, the loop of bacteria is cut open and they will insert the human gene into the plasmid of the common bacteria, E.coli. And the process is the same as the bacteria grow in the media to produce their own protein. After the bacteria produce enough protein, the scientist will isolate the insulin, purify them, and make medicine for patients. This technique is call “Recombinant DNA” ( Library of medicine, 2017). Consequently, by applying the transformation, scientist produce insulin to treat diabetes patients. Moreover, it also decrease the time and the price of the medicine products (Press Releases,