Phagocytosis Lab Report

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Abstract This report presents the performance of phagocytosis in activated macrophage cells, and the comparison of phagocytosis of both wild type J774 cells and Rab5 transfected J774 Eclone Cells. During the experiment, culture dishes where prepared and incubated in 37 degrees, activating macrophages and beginning the phagocytosis process. Phagocytosis was stopped with the addition of 1mL of 3.7% paraformaldehyde fixative at room temperature. Once TritonX (0.1%) was added, pores were created and the nucleus was able to be stained. The staining by DAPI, allows for the number of bacteria inside the cells after the process of engulfing occurs. It was concluded in that the average of THP-1, followed by the Stdev in the specific time frames recorded …show more content…

The protein Clathrin, plays an important role in the pinching and enclosure of the proteins, which are being transported throughout this process of phagocytosis. Phagocytosis by macrophages is important in its role of up taking and degradation of pathogens, as well as immune response. According to another study completed by Ncbi, phagocytosis by macrophages is able to allow the starting process of an immune response. These macrophages enable the process of engulfment of bacteria, basically becoming the kick starter to the entire phagocytosis process (Ncbi, …show more content…

The objective of this was to compare the rates of phagocytosis by counting the phagocytic index between the Rab5- Eclone, and the controlled J774-Eclone cells. By calculating the amount of bacteria inside each cell, bt an observed fluorescent microscope, we are able to calculate the average rate, along with the standard deviation of each time frame. The closer the standard deviation calculations are to 0, the closer it indicated it is near the expected values. Unlike, the standard deviation calculations that are further from their expected values, based on the different time frames. This expresses the significant errors for all THP-1 average rates of phagocytosis

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