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Poly Lactic Acid Synthesis Lab Report

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Metabolic engineered of biocatalyst: A solution for PLA based problems ABSTRACT Poly lactic acid(PLA) is a biodegradable polymer used in many biomedical as well as in packaging Applications. Conventionally, PLA is produced by two method which is direct condensation of lactic acid and ring opening polymerization. The polymer produced from these conventional methods produced polymer which have low molecular weight. Conventional methods of PLA production requires catalyst which makes it unfit for biomedical Applications. Newer method utilizes metabolic engineering for direct production of PLA by fermentation. This can be done by introducing propionate CoA-transferases gene and polyhydroxyalkanoate synthase gene to E.coli for lactyl CoA production. This lactyl CoA by produces by gene manipulation produces poly lactic acid by fermentation. The polymer produced from this method have high quality and increased yield which makes it fit for biomedical applications. key words: biodegradable, E.coli, genetic engineering, lactic acid , lactyl CoA, polyhydroxyalkanoate (PHA), Poly lactic acid INTRODUCTION Polylactic acid (PLA) is rigid thermoplastic polymers that have semi crystalline or totally amorphous geometry, depending on the optical purity of the polymer backbone [1]. Lactic acid has …show more content…

Lactic acid is produced by two methods i.e, chemical method and fermentation method[3]. Chemical method utilizes petrochemical resources followed by addition of HCN and specific catalyst to produce lactic acid [3]. While fermentation methods utilizes renewable resources such as carbohydrate in a fermented broth to obtain lactic acid [3]. Optical purity of lactic acid is very important and hense is major addressed problem in production of PLA. Chemical method produces racemic mixture of both D(-) and L(+) lactic acid while fermentation methods produces only one optically pure form of D(-) or L(+) lactic acid

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Therefore, liquid-liquid and acid-base extraction techniques were successfully performed to separate the components of the Excedrin tablet. According to the TLC analysis results, the compounds (aspirin, acetaminophen, and caffeine) were successfully isolated from the analgesic (Excedrin tablet). In figure 1, the separation of the compound in the TLC analysis correlates with the TLC analysis in figure 2. Furthermore, Rf index calculations of the TLC analysis demonstrated that the compounds (aspirin, acetaminophen, and caffeine) were separated. The Rf calculations of aspirin in table 1 shows an Rf value of .491; however, in table 2 the Rf value of aspirin was calculated to be .784.

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C12H22O11 (lactose) + H2O > 4CH3CHOHCOOH (lactic acid) is the equation for lactose to lactic acid.

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