Methods Radish growth The site of the planting of the cherry-belle radish seeds was located in the basement of my home in Quincy, Massachusetts. During the season of fall, the temperature of Quincy approximates 50°F - 60°F during the day and 30°F - 50°F during the night. As a result, the cherry-belle radishes had to be planted indoors under a ultraviolet growing light. Pots were labeled and separated into four groups: the control group, the ⅙ cup caffeine group, the ⅓ cup caffeine group, and the ½ cup caffeine group. The amount of radish seeds were then evenly distributed among the pots. Approximately four inches of soil was place at the bottom of each pot in order to allow the roots of the radishes to have more room to grow. Then, radish seeds would be place on top of the four inches of soil and would be watered over slightly. About ¼ - ½ inches of soil was used to cover up the seeds and this soil was watered over. Each separated group of pots were water with the corresponding …show more content…
Radishes are not commonly used in this type of experiment. Instead, onions root cells are more commonly used to observe the phases of mitosis under a microscope due to the thin layers that can easily be obtained from it. This leads to the third problem, in which is the inability to obtain thin layers of radish samples during the preparation of microscope slides. The radish, or any other plant, is made up of layers and layers of cells. As a result, if the sample is too thick, too many layers of cells will appear under the microscope and it will become too difficult to observe any cells. In this certain experiment, although some thin layers were obtained for the preparation of the microscope slides, the nuclei and their chromosomes were too small for observation. Due to these issues, no phases of mitosis could be seen within the cells of the radish, thus causing the lack of data being