ANIMAL SELECTION Male albino rats were purchased from authorized supplier of Jabalpur, M.P. weighing 150-200 g. The animals were allowed free access to commercial rat pallet diet (Lipton India ltd. Mumbai ,India) and water add Libitum . Rats were housed in a group of six in clean cages at 250C and 12 hours photoperiod with relative air humidity of 30 to 60% . All the experimental procedures were carried out accordance with committee for the purpose of control and supervision of experiments on animal guide lines .
PREPARATION AND ADMINISTRATION OF DOSES All the doses were prepared in distilled water using 2% tween 80 as suspending agent. The test substances were administered in a single dose using a oral feeding canulla after
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Gas Chromatographic studies on both aqueous and alcohol extract reveles the presence of twenty eight compounds, out of which oleic acid, constitutes the major part. Other major biological compounds include pelargonidin-3-5-diglucoside, cyanidin-3- galactoside, p-hydroxy benzoic acid, gentisic acid, arachidic acid, linoleic acid, palmitic acid, stearic acid, apigenin, apigenin-7-oglucuronide.The seeds show the presence of arachidic acid, cyclopropenoid, linoleic acid, malvalic acid, oleic acid, palmitic acid and stearic acid (Chopra et al, …show more content…
Nadkarni AK. KM. Nadkarni’s Indian Materia Medica Revised and enl arged. Vol I. Bombay: Popular Book Depot;1954.
7.Chopra RN, Chopra DC and Verma BS. Supplement to the Glossary of Indian Medicinal Plants. New Delhi: Council ofScientific and Industrial Research; 1969.
8.Madhava Chetty K. Martynia Annua linnLinn.Chittoor medicinal plants, Himalaya Book Publications, Tirupati, 2005, pp590.
9.OECD, 2002. Acute oral toxicity. Acute oral toxic class method guideline 423 adopted 23.03.1996. In: EleventhAddendum to the, OECD, guidelines for the testing of chemicals organisation for economical co-operation anddevelopment, Paris, June, 2000.
10.Mizui T, Sato H, Hirose F, Doteuchi M. Effect of antiperoxidative drugs on gastric damage induced by ethanol in rats. Life Sciences, 41, 1987, 755 – 763.
11.Rainsford KD, Whitehouse MW. Biochemical gastroprotection from acute ulceration induced by aspirin and relateddrugs. Biochemical Pharmacology, 29, 1980,1281 – 1289.
12.Shay H, Komarov SA, Fels SS, Meranze D, Gruenstein M, Siplet H. A simple method for the uniform production ofgastric ulceration in the rat.Gastroenterology, 5, 1945, 43 –61.
13.Gupta MB, Nath R, Gupta GP, Bhargava KP. A study of the antiulcer activity of diazepan and other tranquillose dativesin albino rats.Clinical and Experimental Pharmacology, 12, 1985, 61 –