ipl-logo

Sfmstr5 Loci Lab Report

1371 Words6 Pages

Polymorphic Markers in Sailfin Molly at the STR5 Loci
Introduction
The purpose of this laboratory report is the explain and analyze the process used to determine the heterozygosity and the allele frequency of the SFMSTR5 loci in Sailfin molly, or Poecilia latipinna. Sailfin molly are a species of fish that inhabit fresh and saltwater bodies of water from South Carolina to Texas. The Sailfin molly examined in this experiment were collected from two different locations in Florida. The fish collected in one location are classified as a single population and the fish collected in the second location are classified as a second population. Population one is composed of eight (8) sailfin molly and population two is composed of five (5) sailfin molly; this gives a total population of thirteen (13) …show more content…

The results of the gel electrophoresis are summarized in Figure 2. On the gel, both populations of fish were run and analyzed for their heterozygosity. Following the conclusion of the electrophoresis, the gel was analyzed to determine how many different alleles were present at the SFMSTR5 loci. The results of the analysis are shown in Figure 2. The gel showed that in population 1, there are three different alleles at the SFMSTR5 loci and that a majority of the fish in this population are heterozygous at this locus. To determine if a fish is heterozygous or homozygous at the SFMSTR5 locus, the number of bands in a particular lane are analyzed. This gel is difficult to analyze due to the fact that it "smiles". When labeling the gel, the allele number is based on where the band is located between the 300 and 400 bp bands on the marker lane. Some of the alleles could be classified as either allele 1 or 2 due to the smiling effect. The classification of the alleles was done by comparing the band location to the location on the marker

Show More
Related
2403 Words | 10 Pages

The Gastrocnemius Muscle of Rana pipiens is an Appropriate Model for Skeletal Muscle Contractile Kinetics When Compared to Peer-Reviewed Models Georgia Institute of Technology BMED 3110: Quantitative Engineering Physiology Laboratory I Section B: Team Baboons 16 November 2014 ABSTRACT The dynamics of skeletal muscle kinetics can be quantified using various experimental methods involving stimulated muscle contractions.

Read More
202 Words | 1 Pages

C4564 Description: IC50: 3-AP is a ribonucleotide reductase inhibitor and iron chelator with antitumor activity. Ribonucleotide reductase, the rate-limiting enzyme for de novo DNA synthesis, is an excellent target for chemotherapy. Its increased activity in cancer cells is associated with malignant transformation and proliferation.

Read More
405 Words | 2 Pages

Discussion 1. Zn0 (s)+ Cu2+S6+O42-(aq) →Cu0(s) + Zn2+S6+O42-(aq) Zn0(s) → Zn2+(aq) + 2e- Cu2+(aq) + 2e- → Cu0(s) Zn0(s) + Cu2+(aq) → Zn2+(aq) + Cu0(s) Oxidant (oxidizing agent) is the element which reduces in experiment.

Read More
574 Words | 3 Pages

Experiment 7 In this experiment we configured several DC circuits consisting of an emf and a network of resistors. The circuits were composed of a power supply, two DMMs, a circuit board, an SPST switch, and an assortment of known resistors along with one unknown resistor. We measured the current and voltage of the entire circuit as well as the potential drops across each resistor to determine the parameters of the circuit including the resistance, voltage, and current for each component.

Read More
791 Words | 4 Pages

In the stickleback fish found in Big Lake (seen in slot #4 and #5) are about 1400 base pairs long. By using PCR, we were successfully able to isolate the Pitx1 enhancer region in the marine control, and both stickleback fish in Big Lake. The sterile water used in the experiment was not contaminated, as seen in slot #6 of the gel electrophoresis, which means the results of the experiment should be accurate and without any contamination. There is a deletion in the Pitx1 enhancer region of the stickleback fish found in Big Lake, since the Pitx1 enhancer region is approximately 117 base pairs shorter than the marine

Read More
727 Words | 3 Pages

This week we went to the Conodoguinet Creek. While we were at the creek we did many different things. One of the experiments we did was the Critter Count which was my favorite. Another experiment we did was the Eutrophication Tests. The last Experiment we did was the bobber test.

Read More
1051 Words | 5 Pages

V. EXPERIMENTAL SETUP & RESULTS The proposed dual T-NPC, dual PMSM topology and its modulation and control strategy are evaluated on an experimental setup as shown in Fig. 13. The experimental setup consists of two three-level T-NPC inverters feeding a dual three-phase 16 pole PMSM. The following capabilities of the proposed topology have been validated: 1) balancing DC-link voltages, 2) reduced output current distortion and 3) reducing capacitor RMS current.

Read More
1233 Words | 5 Pages

Does the amount of blubber an Hourglass Dolphin, Lagenorhynchus cruciger, has depend on the environment it inhabits? Introduction Lagenorhynchus cruciger, also known as the Hourglass Dolphin, is a small dolphin found in the Antarctic and Sub Antarctic waters, which tend to be anywhere between -2 and 10 degrees celsius in temperature. As mammals, they are warm-blooded, produce milk, give birth and take care of their young. The Hourglass Dolphin is black and white in colour, with some shades of grey, this pattern resembles an hourglass. They feed on mainly fish, squid and crustaceans.

Read More
1100 Words | 5 Pages

Introduction A mutation is a heritable change that is passed from the mother cell to progeny cells. Mutations may lead to good, bad or neutral phenotypic changes in the organism. They may occur spontaneously as in random DNA replicative errors or may be induced by mutagenic chemicals or radiation. Besides mutations, another way that bacteria achieve gene diversity is through the three known mechanisms for intercellular gene transfer.

Read More
1117 Words | 5 Pages

Crayfish Response In this experiment the researchers were investigating the limited range of the crayfish species Orconectes saxatilis in the upper part of the Kiamichi River. The dispersal of the crayfish species O. saxatilis and two sympatric crayfish species throughout the upper part of the Kiamichi River were examined. The type of habitat that the crayfish favor for use was also examined along with their historical characteristics. Crayfish species with small habitats are in major need of conservation but little information is known about species-specific habitats.

Read More
794 Words | 4 Pages

There is a higher chance of the eye shape allele of the chromosome to switch as this section is 47.0 map units away from the bristle morphology and even further from the eye color. The body color gene in the F2 data was analyzed using a chi square test to see if the it fit the expected 3:1 Mendelian ratio. There were 168 honey body colored flies observed in the F2 data, and 47 ebony body colored flies. These two numbers were compared to expected values for a 3:1 ratio in order to obtain the X2 value of 1.213. The degree of freedom was determined to be 1 for the data, which is necessary for the interpretation of the X2 value.

Read More
738 Words | 3 Pages

Population genetics is a useful biological tool that allows scientists to not only see genetic variation within a single population, but to also discover inconsistencies between the genetics of two different populations. Comparing the population genetics of two populations can reveal significant differences in their allele frequencies and thus expose meaningful biological variability between the two groups (1). Alleles, or the alternative forms of a gene, are direct indicators of variation within species and populations (1). The relationship between alleles and genotypes can be analyzed using the Hardy-Weinberg Equilibrium (1). The Hardy-Weinberg Equilibrium is a concept in which genetic variation remains constant within a population, if a

Read More
864 Words | 4 Pages

Forces and Newton II Elias Ghantous PHYS 151 – Section NQ Thursday 10:10am Hasbrouck Lab Room 214 October 13, 2017 Abstract In this experiment, I studied how forces cause an object to accelerate. I also studied the relationship between force vectors, mass and acceleration. Gathering of data took place through the use of a force table and a PAScar track system.

Read More
685 Words | 3 Pages

Kautt, Gonzalo Machado-Schiaffino, and Axel Meyer at the University of Konstanz in Germany analyzed the genomes of endemic species of Midas cichlids in two Nicaraguan lakes. The purpose of the experiment was to determine the manner and timeline by which these populations of fish diverged. The researchers studied organisms belonging to the species complex of cichlid fish, Amphilophus. These fish live in two isolated crater lakes, Lake Apoyo and Xiloa, and are endemic to the area. Physiology such as body shape, color, and type of food consumed differentiate each species.

Read More
363 Words | 2 Pages

These fish have become incresingly popular due to the films “Finding Nemo” and “Finding Dory”. Although there is no evidence of population decline thus far, increased demand will cause more fish to be caught, which will decrease populations because there has been no record of successful breeding in captivity (Bradford

Read More

More about Sfmstr5 Loci Lab Report

Related Topics

Open Document