ipl-logo

Spec 20 Lab Results

1279 Words6 Pages

This whole lab was centered on using the Spec 20, performing dilutions, constructing tables and graphs to help us determine the concentrations of phosphate in colas. The Spec 20 is a device that shoots a beam of light through a sample of liquid and measures the amount of light that gets transmitted. How the Spec 20 works is that there is a lamp inside the machine that produces a light through a lens and a slit which forms a narrow beam of light to which this light is directed to a diffraction grating. A diffraction grating is a piece of plastic or glass that can break up light into its different colors. Also, when we change the wavelength, we are basically changing the angle of the diffraction grating. After the light is separated into its …show more content…

These results basically indicated that different colors have different %T and absorbance at different wavelengths. The results for the KMnO4 is also shown in Table 1. Looking at the Chart 3, we determined the analytical wavelength to be 420 nm for KMnO4. With that wavelength in mind, we performed dilutions on 2 x 10-3 M KMnO4 to get %T values between 15 and 85. The concentrations of KMnO4 that gave us %T values between 15 and 85 were 2x10-3 M, 8x10-4 M, 1.6x10-4 M, and 3.2x10-5 M. The purpose of experimenting with the colored solutions and KMnO4 in week one of this experiment was to figure out the relationship between color and wavelength of absorbance. This helped us figure out that the analytical wavelength which is where the absorbance is maximized. We chose the analytical wavelength to be 420 nm because that was where the colors’ absorbance was at its maximum. [Our data does not exactly show 420 nm as the maximum probably due to human error but we were told to use that wavelength since the rest of the class was using that value.] The analytical wavelength is where the absorbance is maximized because that is the place where there is less variability in the values therefore making it more accurate and precise to measure our solution at that wavelength to perform dilutions to get our desired %T …show more content…

We were given 3 samples of colas, Dr. Thunder, Diet Sam’s Cola, and Mountain Dew Code Red. First, the colas had to be decarbonized by heating the colas, carefully, making sure to not bring it to a boil as that would affect the concentration. The CO2 had to be removed because it can also affect the concentration. Then, AVM had to be added to the colas because we added it to the phosphate solution in week two. The AVM diluted the concentration of the phosphate solution, so we must add it to the colas also to accurately measure the phosphate content since we are using the analytical wavelength of 380 nm from the phosphate in week 2 to measure the %T of the colas. Dr. Thunder had a %T of 45.29, Diet Sam’s Cola had a %T of 61.09, and Mountain Dew Code Red had a %T of 64.7. Then, the concentration of phosphate was determined in the cola using the calibration curve which is basically a graph of concentration vs %T with a regression equation as shown in Chart 6. The regression

Show More
Related

Catechol Oxidase Lab Report

642 Words | 3 Pages

Record the amount of absorbance by converting transmittance every 5 minutes for a total of 20 minutes. Repeat all of these steps for the cantaloupe, banana, replacing the blank each time to recalibrate the spectrophotometer. After recording all the percent transmittance value, the data was then converted into absorbance value by using the absorbance conversion table. The information was placed on a plotted graph

Read More

Equilibrium Lab Report

1249 Words | 5 Pages

Empty the blank and use the solution from test tube one to rinse the cuvette twice. Fill it ¾ with solution one, wipe the outside, and place it in the spectrometer. 8. Start data collection and display a full spectrum graph. Stop the data and the wavelength of maximum absorbance will be identified.

Read More

Unknown Compounds

1027 Words | 5 Pages

Each buffer was measured in a 100 mL graduated cylinder and contained in a 40 mL beaker. Once the reading of the buffer was stabilized, the program entered into reading 1. The probe was cleaned with distilled water and dried before being placed into the second buffer for reading 2. Once the second calibration was completed the pH probe was cleaned again. Next the probe was placed into the unknown solution.

Read More

Iron Chloride And Ascorbic Acid Lab Report

522 Words | 3 Pages

The test tube was swirled to mix the solution. A small amount of ascorbic acid was added to the test tube. The exact amount of ascorbic acid needed in the solution varied from .0002 grams and .00002 grams, but the electronic scale only weighed to the nearest .01

Read More

Red Food Test Lab Report

1035 Words | 5 Pages

Blue food coloring measuring 629.2nm at 1000ppm. The blue food coloring had the highest spectrum of all the colors measured. Through placing fixed solutions into a spectrophotometer, light absorbance can be determined for each color. Being careful with this process, a few errors that could occur would be cross contamination of the solutions or fingerprints on the cuvettes. By measuring these colors, the blue food coloring had the highest

Read More

Chemical Reaction Lab

1525 Words | 7 Pages

When concentration increased, absorbance increased, and when concentration decreased absorbance decreased as seen in the graph of Concentration vs Absorbance. Equation 1 〖rate〗_1/〖rate〗_2 =(k〖[Dye]〗^n 〖[NaOH]〗^m)/(k〖[Dye]〗^n 〖[NaOH]〗^m ) For all the solutions, the rate of decay of dye was linear on a ln[A] vs time graph with a slope of -k, thus indicating the rate order for the dye as first order4.

Read More

Chloroplast Membranes Lab Report

1291 Words | 6 Pages

Wavelength A graph was plotted on MS Excel with absorbance on the y-axis and wavelength (nm) on the x-axis. The absorption rate initially increased until the peak of 440 nm was reached (see Figure 1). After the decline of the first peak, the rate increased until the next peak was reached at 670 nm. The peak absorbance region was at 440 nm with an absorption rate of about .818 and at 670 nm with an absorption rate of about .431. Thus, the highest absorbance values were reached at the wavelengths 440 nm and 670 nm.

Read More

Ammonium Sulfate Lab Report

512 Words | 3 Pages

The spectrophotometer was zeroed on the computer at 595nm using this blank as the reference. Then one by one each cuvette with the different diluted protein solution was placed in the spectrophotometer and their absorbance was recorded. This was then plotted as a standard curve showing absorbance (y-axis) and protein in micrograms (x-axis). The x-axis of this curve was calculated based on the concentration ~0.3mg/mL of the original protein standard

Read More

Density Exploration Lab

1118 Words | 5 Pages

I calculated 11mL, 15mL and 20mL of the diet, unsweetened Coca-Cola. When calculating the density I used the same density formula that was used to calculate the density of the copper. I used two volumetric pipets for each measurement and did three trials for each of the three different volumes, accounting for a total of nine measurements. From our findings we calculated that the average density of the diet Coca-Cola was 1.026256481 g/mL while the traditionally sweetened or regular coke had a density of 0.9612834659 g/mL. This was very surprising to me as I thought that the density of the regular Coca-Cola would have been more due to the different types of sweeteners used. The data collected also did not seem consistent with the water test when the two cans were put into water.

Read More

Determining The Concentration Of Unknown Feii Salt

1055 Words | 5 Pages

M1V1= M2V2 (2) Equation 2 was used to account for all the dilutions that were performed during this experiment in order to be within range of the calibration curve produced in Figure 1.2. M1 is

Read More

How Does The Concentration Of Distilled Water Affect The Rate Of Photosynthesis Lab

1364 Words | 6 Pages

The results of each statistical calculation (sample size, mean, calculated t-values, degrees of freedom and p-values) are displayed in Table 1. Mean is calculated by the summation of a data set followed by division by the sample size. This is repeated for each set of treatment data as well as the control (dH2O). The calculated t-values are derived from the absolute value of the difference between the mean of distilled water and the treatment, divided by the square root of the summation of both squared SEM values. The values used for distilled water remain the same throughout the data.

Read More

Visual Testing Lab Report Results

285 Words | 2 Pages

_ The temperature was measured and it was 27 degrees celsius, the compound was dissolved and the test of PH was done at the

Read More

Iced Tea Experiment

1187 Words | 5 Pages

 Put the caps on the bottles and shake them until all the sugar is dissolved. Testing the Sugar Solutions  All of the solutions should be the same temperature before testing  Place the hydrometer in the 0% sugar bottle, record your reading in your notebook  Repeat this step for the 5%, 10%, 15%, 20% and 25% sugar solutions.  Rinse and dry the hydrometer between readings. Testing the Soda and Iced Tea  Place the hydrometer in soda 1(coke).

Read More

Limiting Reactant Lab Report

1438 Words | 6 Pages

Purpose In this experiment, the limiting reactant of a chemical reaction and the percent by mass composition of a mixture were determined. An unknown salt mixture containing sodium phosphate and barium chloride dihydrate was added to water producing a mixture containing a solid precipitate of barium phosphate. Through the use of filtration, the precipitate was removed from the mixture completely. The filtered liquid was transferred into multiple test tubes, each test tube had either barium chloride or sodium phosphate added into the liquid.

Read More

Phenol-Sulfuric Acid Analysis Essay

740 Words | 3 Pages

A significant portion of the monosaccharides in soda are fructose, and, therefore, an assumption regarding the correlation that a standard curve generated from a glucose solution has with an unknown soda sample should not be made. It would be more appropriate to use a solution with approximately 1:1 ratios of fructose and glucose when constructing a standard curve for the phenol-sulfuric acid analysis of soda. This is approximately the ratio that is witnessed in commercial HFCS; therefore, it would create a standard curve that correlates more robustly with the soda

Read More

More about Spec 20 Lab Results

Open Document