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The Anthraquinone Dye Experiment

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The anthraquinone dye experiment has the purpose to identify the anthraquinone dyes from unknown mixture by using thin layer chromatography (TLC) of the unknown fraction. An anthraquinone is an aromatic organic compound obtained by the oxidation of anthracene. To separate the compounds in the mixture, column chromatography and thin layer chromatography uses portioning of a sample between a stationary solid phase and a liquid mobile phase. As the stationary phase, they use either silica gel or alumina, and organic solvents as the mobile phase. In order to accomplish the experiment, an unknown which is a solution of at least two anthraquinone dyes will be used. Then, a column chromatography is used to separate the components of the mixture into individual fractions. After that, a thin layer …show more content…

Acetone is a high flammable liquid that caused serious aye irritation and may cause drowsiness and dizziness, and irritation to mucous membranes. Dichloromethane – when in contact with skin, eyes, or respiratory tract might cause irritation; if swallowed is harmful and may cause problems in the nervous system.

A thin layer chromatography chamber and TLC plate with silica were prepared; a line was drawn with pencil about 1 cm above the bottom of the plate. Using a capillary tube, small drops of solvent were added to the plate leaving then the solvent evaporate (the smaller the diameter of the spot, the more accurate the results). Methylene chloride was added to the TLC chamber until it reaches 0.5 cm depth in order to cover the bottom of the jar; a piece of filter paper was added to the jar allowing the solvent to travel up the paper and the surface area of the solvent increased. Then the plate was placed in the jar containing 100% CH2Cl2 so that the top of the plate rested against the side of the jar opposite the filter paper. When the eluent was near to the top of TLC plate, the plate was removed and then

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