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Thermocycler Lab Report

1520 Words7 Pages

TAS tubes we used TAS2R38F and TAS2R38R. Each primer is complementary to one strand of the DNA, and these primers allowed for instant DNA amplification of a certain DNA sequence at a specific site. Each student then added their cheek cells to their respective tubes, and then they would be centrifuged and then placed in a thermocycler. The thermocycler had pre-programmed temperature to denature, anneal, and synthesize the DNA. After completing the PCR reactions, we placed our tubes into a freezer(Leight and McAllister 2017). Every student grabbed their specific tube and let it thaw out. Each student then obtained a 5 mL microtube that would be filled with a 5 microliters of a certain PCR product, 10 microliters of its enzyme cocktail, and …show more content…

This graph simply breaks down the observed genotypes, and then we use the Hardy-Weinberg Equilibrium equation to predict the expected individuals that will end up with a certain genotype/ For both tables we calculated a P-value, and that calculation was previously described above. If the P-value was below .05 than we failed to reject the hypothesis, and if the P-value was greater than .05 we would reject the null hypothesis. In this experiment, there was only one degree of freedom which corresponded to 3.84 being equivalent to a P-value of .05. So if we calculated a chi-square value above 3.84 we would reject the null hypothesis. If the chi-square value was less than 3.84 we would fail to reject the null hypothesis. After calculating, we found the LCT loci to have a chi-square value of 20.97, which definitely exceeded our necessary value of 3.84, so we were able to reject the null hypothesis for this allele. As for the TAS2R38 allele, we calculated a chi-square value of 2.78, which fell under the necessary value of 3.84, thus we would fail to reject the null for this

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