Exploring K-RAS^G12D Mutations and Tumorigenesis in Mice
School
Northwood High School**We aren't endorsed by this school
Course
CANCER RESEARCH 1
Subject
Biology
Date
Dec 11, 2024
Pages
11
Uploaded by PresidentProtonQuetzal41
Studying K-RAS^G12D mutations in miceBy: Eldaah ZelalemNCI Summer Intern
Examine the effects of K-RAS^G12D mutations in mouse embryonic fibroblastsIs a single mutation enough to promote tumorigenesis?●What is known?: K-RAS^G12D with it’s mutations is found in at least 20% of all types of human cancers. ●Answer: No, a single mutation of K-RAS^G12D is not enough to cause a mutation in a mouse embryonic fibroblasts.
HypothesisI do not believe a single mutation will promote tumorigenesis.
MethodsIn this process the method used was cell collection. By looking at the cells within the mouse embryonic fibroblasts we were able to study & observe the effect K-RAS^G12D had on the mouse.
Cell Morphology
Growth curve
Soft Agar Assay
Data analysis Our data slowly grew at around the same pace until day 5 through 17. That is when we see the different mutations begin to largely differ from one another.
Strength’s●We weren’t able to physically perform the tasks ourselves●The methods & techniques used to collect data ●Time constraintLimitations●The potential depth●The amount of previous data available regarding this topic●The patterns of our data can be hard to see which makes displaying the differences between the growth curves visible
ConclusionTo conclude, my overall findings are that it is not possible for a single mutation to promote tumorigenesis because cancer only forms through multiple mutations. This proves my hypothesis was correct.
AcknowledgmentsThank you NCI Thank you Chanelle Chase Borden, Ph.D & Mary Grace Katusiime Ph.DWang, Zuoyun, et al. “Temporal Dissection of K-RasG12D Mutant In Vitro and In Vivo Using a Regulatable K-RasG12D Mouse Allele - PMC.” PubMed Central (PMC), https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3350485/. Accessed 19 July 2022.