Understanding Gene Editing Techniques: Key Concepts and Methods
School
University Of Connecticut**We aren't endorsed by this school
Course
ANSC 3323
Subject
Biology
Date
Dec 12, 2024
Pages
1
Uploaded by ChancellorStraw15059
AS3323/5621 Worksheet 24, 11/21/2024 Print name on backQ1. T/F. Frame shift is functionally knocking a gene out. Q2. T/F. DNA fragment insertion, base pair changes, small insertion and deletion can all happen naturally.Q3: what is the most important difference between these two methods?The presence or absence of a DNA-cutting enzyme.Q4: what is the role of the guide RNA?Bring the CAS9 to the right location.Q5: Can CAS9 cut anywhere on DNA and at any time? When can it cut?No, it needs to bind to a guide RNA. Q6. T/F. The delayed genetic changes will generate chimeras. Q7. T/F. SCNT using transfected cells will generate mosaics. Q8. What does blastocyst injection generate? a) chimeras, b) mosaics. Q9. What embryonic technology can avoid breeding ? Tetraploid complementation. Q10: what are the three ways to create gene-edited animals?Microinjection (pronuclear injection/cytoplasm injection), cloning (SCNT), blastocyst injection.
