Understanding CAC and PPP: Key Concepts and Reactions
School
Vincent Massey Secondary School**We aren't endorsed by this school
Course
BIOLOGY SBI4UC
Subject
Chemistry
Date
Dec 12, 2024
Pages
4
Uploaded by tayeba4
Quiz CAC 2024 and PPP 241. Gofor malate dehydrogenase reaction of CAC is +29.7KJ/Mol. Malate + NAD+= Oxaloacetate + NADH a. Which way will this reaction go in standard condition, forward or backward?b. How does this reaction proceeds in forward direction?c. Calculate the G for this reaction in forward direction if concentration of oxaloacetate is 1x 10-8M, malate 0.2 mM, and NAD+/NADH ratio in rat liver mitochondria is 10.2. a. What enzymes are activated by insulin-stimulated protein kinase in glucose metabolism? b. Insulin generally promotes anabolic pathway i.e. synthesis of glycogen then why should it activate PDC?3. What side reaction would take place if E1 subunit is separated from PDC?4. Which of the dehydrogenases have FAD cofactor?
5. How many oxygen molecules are used for the complete oxidation of glucose to CO2during Glycolysis, PDC and citric acid cycle pathway?6. Where do the additional 6 oxygen atoms come from, to generate 6 molecules of carbon dioxide? 7. How many grams of glucose is needed to elevate an object of 50 Kg to the height of 100 meters. Assume that all the energy released by ATP hydrolysis is used with 100% efficiency in this work, and G = - 50KJ/Mole in physiological condition. 8. Deficiency of thiamine or arsenate poisoning leads to the similar symptoms andmostly brain functions are affected in these diseases. Why? 9. Which enzymes are affected by thiamine deficiency?10. How many net ATP molecules are produced by substrate level phosphorylation during complete catabolism of one glucose molecule?11. CAC is referred as amphibolic pathway Why?12. What is the role of E3 enzyme in PDC reaction mechanism?13. Indicate the Nucleophilic Substitution steps in PDC mechanism.
Pentose Phosphate pathway 2024:1.What are the end products of pentose phosphate pathway?2.What is the need for pentose phosphate pathway?3.People with defective GPDH enzyme are resistant to malaria. Why? 4.What enzymes are responsible for the production of NADPH and pentose phosphate?5.What are the other enzymes involved in pentose phosphate pathway? Why are they needed in this pathway?6.Which of the following is a correct statement?a.Pentose phosphate pathway can be directly linked to CAC.b.Pentose phosphate pathway can be directly linked to glycolysis.c.Pentose phosphate pathway can be directly linked to glycogen metabolism.d.None of the above is correct statement.
7.What are the substrates and end products of pentose phosphate pathway? 8.Why are people with defective Glucose 6 Phosphate dehydrogenase (G6PDH) are resistant to malaria?9.Hexokinase is upregulated (i.e. present in high amount) in cancer cells?