Practical SEM Manual and Rubric
.pdf
School
University Malaysia Sarawak**We aren't endorsed by this school
Course
STB 1013
Subject
Biology
Date
Dec 29, 2024
Pages
9
Uploaded by UltraPolarBearMaster1162
1 1STB1013 Cell Biology Laboratory Manual: Scanning Electron Microscopy (SEM) Introduction Welcome to the Cell Biology laboratory demonstration on Scanning Electron Microscopy (SEM). In this session, you will have the opportunity to explore the principles, techniques, and applications of SEM in cell biology research. This manual will guide you through the process of the laboratory demonstration and help you prepare a comprehensive lab report that showcases your understanding of the topic and your abilities in leadership, autonomy, and responsibility. Objectives 1.To understand the principles and working mechanisms of SEM. 2.To observe and analyze biological specimens using SEM. 3.To learn the importance of leadership, autonomy, and responsibility in a laboratory setting. Equipment and Materials •Scanning Electron Microscope (SEM) •Biological specimens (provided by the instructor) •Safety goggles •Lab coats •Gloves •Notebooks and pens Safety Precautions 1.Always wear safety goggles, lab coats, and gloves when working in the laboratory. 2.Follow all safety instructions provided by the instructor. 3.Avoid touching your face or eyes while working with specimens. 4.Notify the instructor immediately in case of any accidents or spills. Laboratory Demonstration: Scanning Electron Microscopy (SEM) 1.SEM Principle and Operation:•The demonstrator will provide a brief overview of SEM principles and how the microscope operates. •Pay close attention to the instructor's explanations and ask questions if needed. 2.Sample Preparation:•Observe the demonstrator's demonstration of specimen preparation for SEM. •Note the steps involved in fixing, dehydrating, and coating the sample. 3.SEM Imaging:•Work in groups of 5 students. •Each group will have the opportunity to load a prepared specimen into the SEM.
2 2•Observe the SEM images of the specimen and discuss your observations with your group. 4.Data Collection:•Take notes on the SEM images, magnification settings, and any interesting features you observe. •Discuss with your group members and gather data for your lab report.
3 3Lab Report Instructions PART 1: WRITTEN REPORT 1.Your lab report should be typed in Font 12 and organized neatly. 2.Include a cover page with the following information: •Title: "SEM Laboratory Report" •Your name and the names of your group members. •Date of the laboratory demonstration. 3.The report should be structured as follows: •Briefly introduce the purpose and objectives of the laboratory demonstration. 4.SEM Section •Describe the principles of SEM. •Discuss the sample preparation process. •Present SEM images and analyze the observed features. 5.Discussion •Comparing SEM with Other Microscopy Techniques (10%) •Discuss the significance of the observed features in the context of cell biology. 6.Conclusion •Summarize the key findings and their relevance. 7.References •Cite any sources you used to prepare your report.
4 4PART 2: VIDEO REPORT on SEM Demonstration with Emphasis on Leadership, Autonomy, and ResponsibilityIn Part 2, you will create a video report that highlights your experience during the SEM (Scanning Electron Microscope) demonstration with a specific focus on demonstrating leadership, autonomy, and responsibility. Here are the instructions for this part:I. Introduction to the Video Report (10%) Begin by introducing yourself and your group members. Clearly state the purpose of the video: to share your SEM experience and how it showcases leadership, autonomy, and responsibility.II. SEM Demonstration Overview (60%) In this section, visually and narratively convey your experience with the SEM demonstration, emphasizing leadership, autonomy, and responsibility. It comprises various elements: A. Leadership (15%) Showcase leadership roles taken by group members.Highlight decisions made and their impact on the group's success.Provide examples of effective leadership during the SEM demonstration.B. Autonomy (15%) Highlight instances where you and your group members demonstrated autonomy in decision-making.Discuss the importance of autonomy in scientific research.Explain how autonomy contributed to your group's effectiveness.C. Responsibility (15%) Demonstrate how responsibility was upheld in tasks, safety measures, and group collaboration.Share examples of responsible behavior and its significance in a scientific context.Highlight how the division of responsibilities contributed to the group's success.D. Reflection on SEM Experience (15%) Reflect on your overall experience during the SEM demonstration, with a focus on leadership, autonomy, and responsibility. Discuss the lessons learned and how they will impact your future studies and career.III. Comparing SEM with Other Microscopy Techniques (10%) Provide a simple visual comparison of SEM with other microscopy techniques.Explain the advantages and limitations of SEM, emphasizing its significance in your scientific work.IV. Conclusion and Future Implications (10%)
5 5Summarize your main points regarding leadership, autonomy, and responsibility.Discuss the implications of this experience on your future studies and career.V. Closing and Acknowledgments (10%) Conclude the video report, summarizing the key points.Express gratitude to your viewers for watching the report and acknowledge any resources, instructors, or group members who contributed.
6 16Rubric for Part 1: Written ReportSectionCriteriaExcellent (5)Good (4)Satisfactory (3)Needs Improvement (2)Inadequate (1)Absent (0)Introduction (10%)- Clarity and significance Exceptional clarity, highly significant Clear, significant Mostly clear, somewhat significant Limited clarity, minimal significance Unclear, no significance Absent SEM Demonstration (60%)- SEM Instrument Operation (15%) Thorough explanation and detail Clear explanation Adequate explanation Limited explanation Incomplete explanation Absent - Sample Preparation Exercise (15%) Comprehensive description Clear description Adequate description Limited description Incomplete description Absent - Image Capture and Analysis (15%) Detailed explanations and insights Clear explanations Adequate explanations Limited explanations Incomplete explanations Absent - Reflections on SEM Experience (15%) Deep and insightful reflections Clear reflections Adequate reflections Limited reflections Incomplete reflections Absent Comparing SEM with Other Microscopy Techniques (10%)- Clarity and depth of comparison Thorough, insightful comparison Clear comparison Adequate comparison Limited comparison Superficial comparison Absent
7 17Conclusion and Future Implications (10%)- Summary and implications Exceptional summary and forward-thinking implications Clear summary and implications Adequate summary and implications Limited summary and implications Incomplete summary and implications Absent References (10%)- Proper citations Properly cited references Mostly proper citations Some citations but with errors Few citations with errors No citations Absent Total (100%)
8 18Rubric for Part 2: Video Report with Emphasis on Leadership, Autonomy, and Responsibility Section Criteria Excellent (5) Good (4) Satisfactory (3) Needs Improvement (2) Inadequate (1) Absent (0) Introduction to the Video Report (10%) Clarity and engagement Exceptionally clear and engaging Clear and engaging Mostly clear and somewhat engaging Limited clarity and minimal engagement Unclear, no engagement Absent SEM Demonstration Overview (60%) Leadership (15%) Outstanding demonstration of leadership Clear demonstration of leadership Adequate demonstration of leadership Limited demonstration of leadership Incomplete or unclear demonstration of leadership Absent Autonomy (15%) Outstanding demonstration of autonomy Clear demonstration of autonomy Adequate demonstration of autonomy Limited demonstration of autonomy Incomplete or unclear demonstration of autonomy Absent Responsibility (15%) Outstanding demonstration of responsibility Clear demonstration of responsibility Adequate demonstration of responsibility Limited demonstration of responsibility Incomplete or unclear demonstration of responsibility Absent Reflection on SEM Experience (15%) Deep, insightful reflections on leadership, autonomy, and responsibility Clear reflections on leadership, autonomy, and responsibility Adequate reflections on leadership, autonomy, and responsibility Limited reflections on leadership, autonomy, and responsibility Incomplete or superficial reflections on leadership, autonomy, and responsibility Absent Comparing SEM with Other Microscopy Techniques (10%) Clarity and depth of comparison Thorough and insightful comparison Clear comparison Adequate comparison Limited comparison Superficial comparison Absent Conclusion and Future Implications (10%) Summary and implications Exceptional summary and forward-thinking implications Clear summary and implications Adequate summary and implications Limited summary and implications Incomplete summary and implications Absent
9 19Closing and Acknowledgments (10%) Closure and acknowledgments Clear and appropriate closure and acknowledgments Clear closure and acknowledgments Adequate closure and acknowledgments Limited closure and acknowledgments Incomplete or inappropriate closure and acknowledgments Absent Total (100%)