AB LAB 1322
.pdf
School
Harvard University**We aren't endorsed by this school
Course
BIOLOGY E2B
Subject
Biology
Date
Jan 14, 2025
Pages
2
Uploaded by BrigadierBravery1227
Antibiotic Lab Question: How do different antibiotics affect the growth of bacteria species, and how can the reaction determine if the bacteria is resistant or sensitive to each antibiotic? Claim: Evidence: Antibiotic Diameter of clear area (mm) after 24 hours. E. coli Is Bacteria Resistant or Sensitive to the antibiotic? Diameter of clear area (mm) after 24 hours. Staph Is Bacteria Resistant or Sensitive to the antibiotic? 1: Tetracycline (TE) 2: Neomycin (N) 3: Chloramphenicol (C) 4: Kanamycin (K) 5: Penicillin (P) 1. 20 mm 2. 20 mm 3. 25 mm 4. 25mm 5. 0mm 1. Sensitive 2. Sensitive 3. Sensitive 4. Sensitive 5. Resistant 1. 38 mm 2. 25 mm 3. 30 mm 4. 40 mm 5. 0 mm 1. Sensitive 2. Sensitive 3. Sensitive 4. Sensitive 5. Resistant
Reasoning: How do antibiotics work? Sensitive or resistant? Narrow or Broad spectrum? As much antibiotic vocab as possible. Zone of inhibition? Antibiotics work by targeting specific structures in bacteria, such as cell wall synthesis (e.g., Penicillin) or protein synthesis (e.g., Tetracycline), allowing them a uniquely diverse way to inhibit growth of bacteria. Sensitivity to an antibiotic is indicated by a measurable zone of inhibition, where bacteria fail to grow, while resistance occurs when the bacteria grows despite presence of the antibiotic. The diameter of the clear area around the antibiotic disc determined our sensitivity, and what was present presented a narrow-spectrum relationship. For instance, narrow-spectrum antibiotics, specifically Penicillin, target Gram + bacteria efficiently, bridging the sensitivity seen with Staphylococcus. Broad-spectrum antibiotics like Tetracycline can affect both Gram + or - and again proved to show resistance in Staphylococcus. The antibiotic’s unique relationship with specific bacteria emphasizes how a variety is needed to treat individuals struggling with the sickness that follows. How do they become resistant? What do they use to become resistant? Resistance. How do they maintain it? How do they keep it?