Gram Essays

unknown bacteria. Gram Stain Gram stain is a common technique used to distinguish between gram positive and gram negative. Gram positive bacteria will be carrying a purple color of the crystal violet stains; whereas, gram-negative bacteria carry a hot pink color of the counterstain safranin. According to the result, the unknown bacteria are bacilli in a single arrangement. Their sizes are estimated at 2 micrometers wide. The

unknowns the tests I conducted were the Gram Stain Test, Endospore stain test, Urease test, Lactase test, and lastly the MRVP Methyl Red test. The first test conducted was the Gram Stain test. This test was used to determine if the two bacteria were either Gram Negative or Gram Positive. There are four main steps in the gram stain process that all affect the outcome and overall stain and accuracy of the test. The Primary stain is the first step in the gram stain. This process includes staining the

the plate for 24 hours. The second step performed was the Gram Stain test. The Gram Stain was used to determine whether the unknown bacterium was gram-positive or gram-negative. First, the unknown sample was smeared onto a slide along with a drop of distilled water. Second, the unknown was air dried and was heat fixed. Third, a series of stains/solution such as crystal violet, iodine, ethanol, and Safranin were used to complete the Gram Stain. During this process each stain/solution was left on

In experiment three, Gram staining was used to characterize bacteria, either gram positive or gram negative. The Bacillus slide was Gram positive for the results displaying that it had a strong affinity for crystal violet on applying the iodine. The Bacillus slide stained the color of violet because the thicker and richer the layer of the peptidoglycan walls, the more retention of the stain. While the Pseudomonas slide stained the color of red. The Pseudomonas slide was Gram negative due to the lack

Christian Gram was a bacteriologist who wanted to find a procedure to make bacteria that previously difficult to see under microscope, appear with ease. Gram became a pioneer in the microbiology field, because of his staining technique that he used on bacteria to increase their visibility in 1884. This staining method is called Gram Staining. The Gram stain is a differential stain where a decolorization step occurs due to two different basic stains. The Gram stain use a primary stain of crystal violet

Introduction: This experiment was performed to identify the unknown Species given, using selective and differential media. Materials and Methods: To begin this experiment, The Gram stain procedure will help identify a Gram-positive or Gram-negative bacteria. It begins with grabbing a clean glass slide and placing it on the glass staining rack. Flaming the inoculating loop sterile, use the DI water to create a bubble of DI water in the loop and placing it the center of the glass slide. Then flaming

Discussion and Conclusion The Gram positive Unk. A-DB was identified to be B. Cereus. This inference was reached though quite a bit of study. Firstly the Gram positive was found to be Bacilli shaped this eliminated the gram negative bacteia and three of the nine possible bacteria, leaveing B. cereus, B. megaterium, B. subtilis, C. diphtheriae, M. phlei and M. smegmatis. A timperature test was proformed so as to eliminate the two bacteria whose optimale temperature is 30°(alt+0176) C or exclude

Abstract This experiment was carried out to determine the species of the unknown organism. Once a choice of the unknown was made a Gram stain was conducted to determine the gram nature and morphology of the organism which was Gram negative bacilli. Based on those results a citrate utilization test was performed resulting in a positive test. Following the flow chart the next test to conduct was a motility test which also had a positive outcome. Lastly, a glucose fermentation test was conducted to

Bacteria from the provided master stock plate were used for the gram stain test. Bacteria were then streak and grown from the master stock plate onto a working plate. After an incubation time of 24 hours at 37°C, the working plate bacteria were then used to perform the catalase and red blood cell hemolysis tests. After conducting the three tests, it was concluded that unknown #5 was Streptococcus agalactiae. Gram Stain Test The Gram Stain test was first performed to differentiate the bacteria based

Primary root canal infections are polymicrobial, typically dominated by obligatory anaerobic bacteria. The most commonly isolated microorganisms before root canal treatment include Gram-negative anaerobic rods, Gram-positive anaerobic cocci, Gram-positive anaerobic and facultative rods, Lactobacillus species and Gram-positive facultative Streptococcus species.83 The obligate anaerobes are rather easily eradicated during root canal treatment. In contrast, facultative bacteria such as non-mutans Streptococci

The main purpose of this study is to identify the given unknown bacteria by gram staining and a series of biochemical test. There are many types of bacteria and they produce different enzymes. However, Some bacteria produces same enzymes, so specific tests were done to identify the particular enzymes. There are four main types of Gram-negative bacteria, which are Escherichia coli, Klebsiella pneumonia, Enterobacter aerogenes, and Salmonella typhimurium. Therefore, the unknown could be any of these

Introduction. Christian Gram created the Gram stain technique in 1884 (K. Rudolph [Clemson University, Clemson, SC], personal communication). Gram used tissue in his first attempt with staining (1). The staining of the organisms allowed for the viewing of structure and determining those components by the viewing of the color that appeared after staining the organism (2). The purpose of this experiment was to identify the organism as gram-positive or gram-negative. The first step in the gram staining procedure

The gram stain was done to identify whether the organism was a gram positive or gram negative organism. A few colonies were selected from the nutrient agar. The steps of the gram stain were executed. The stain resulted in gram negative rods. This result eliminated all gram positive organisms. Leaving the possible organisms to be Escherichia coli, Enterobacter aerogenes, Proteus mirabilis

Excitement in Gram Gram is an extremely enthusiastic person which is definitely shown in the passage. There are certainly a couple ways you can tell. You can tell by her personality and her beliefs, and how the author portrays Gram’s beliefs in the story. It also shows how the two things are very alike. Hopefully Gram’s beliefs won’t get her in too much trouble! Gram has an exciting and spunky personality. When you start reading further in the story you can start to recognize what Gram’s belief

Fast Food Noura Adel Al Bader Gust University for Science and Technology Each day one in four Americans visit a fast food restaurant. If you regularly eat fast food it will diminish your health and all consumers should be more aware of that. Every fast food restaurant should put a sign that shows the harmful effect it has on the wellbeing of a human being. Taking care of what you eat should be part of your lifestyle. Fast food advertisements should be strictly controlled, consuming it

CONCLUSION When you put an egg in vinegar, we see that the shell dissolves, but do you ever wonder why? An egg is made mostly out of calcium carbonate which reacts with an ingredient in vinegar, acetic acid. Acetic acid is about 4% of the vinegar and what breaks apart the solid calcium carbonate crystals. The bubbles we see, from the egg, is the carbonate that make carbon dioxide and the other calcium ions float free. This is the equation: CaCO3 (s) + 2 HC2H3O2 (aq) → Ca(C2H3O2)2 (aq) + H2O

energy is more stable than a compound with higher potential energy. Chapter 7, also discusses the focus on the human body energy in nutrition. This section talks about caloric value of the substance, and how one gram of protein are one gram of carbohydrate typically releases about four cal/gram etc. And how much energy is provided from this. Chapter 7 also discusses energy changes in reactions, and how breaking a bond requires energy. Bond

completed in lab, a basic understanding of each type of bacteria was used to create a flow chart that would aid the process of identifying the unknown bacteria within the culture. A gram stain that is performed initially will narrow down the types of tests certain bacteria will and will not respond to. In addition to the gram stain, some of the tests that were used include, a catalase test, an Eosin methylene blue (EMB) agar test, a bile esculin test, and a 6.5% sodium chloride (NaCl) test. If the catalase

through to be able to correctly identify what bacteria was given. It is important to correctly identify the bacteria because some bacteria are more harmful than others. The gram stain is the first test that should be performed because it helps narrow down the possibilities by telling one whether the bacteria is gram positive or gram negative. After this test is performed, one shall place bacteria on/in Mannitol Salt agar, MacConkey agar, Eosin Methylene Blue agar, Urea agar, Simmon’s Citrate, Purple

The bacterium was determined to be Gram- negative rods. The first attempt of gram stain the unknown appeared to look positive and cocci. But, the next day onwards for 4 consecutive days the unknown showed up as a gram- negative gram stain and appeared pink rods. Triple Sugar Iron Agar is a differential medium. It differentiates Gram-negative enteric bacteria based on the ability to reduce sulfur and ferment carbohydrates (lactose, sucrose, and glucose). This is indicated by the formation of acid