Gram staining Essays

Introduction: This experiment was performed to identify the unknown Species given, using selective and differential media. Materials and Methods: To begin this experiment, The Gram stain procedure will help identify a Gram-positive or Gram-negative bacteria. It begins with grabbing a clean glass slide and placing it on the glass staining rack. Flaming the inoculating loop sterile, use the DI water to create a bubble of DI water in the loop and placing it the center of the glass slide. Then flaming the unknown

In experiment three, Gram staining was used to characterize bacteria, either gram positive or gram negative. The Bacillus slide was Gram positive for the results displaying that it had a strong affinity for crystal violet on applying the iodine. The Bacillus slide stained the color of violet because the thicker and richer the layer of the peptidoglycan walls, the more retention of the stain. While the Pseudomonas slide stained the color of red. The Pseudomonas slide was Gram negative due to the lack

Christian Gram was a bacteriologist who wanted to find a procedure to make bacteria that previously difficult to see under microscope, appear with ease. Gram became a pioneer in the microbiology field, because of his staining technique that he used on bacteria to increase their visibility in 1884. This staining method is called Gram Staining. The Gram stain is a differential stain where a decolorization step occurs due to two different basic stains. The Gram stain use a primary stain of crystal violet

The main purpose of this study is to identify the given unknown bacteria by gram staining and a series of biochemical test. There are many types of bacteria and they produce different enzymes. However, Some bacteria produces same enzymes, so specific tests were done to identify the particular enzymes. There are four main types of Gram-negative bacteria, which are Escherichia coli, Klebsiella pneumonia, Enterobacter aerogenes, and Salmonella typhimurium. Therefore, the unknown could be any of these

Christian Gram created the Gram stain technique in 1884 (K. Rudolph [Clemson University, Clemson, SC], personal communication). Gram used tissue in his first attempt with staining (1). The staining of the organisms allowed for the viewing of structure and determining those components by the viewing of the color that appeared after staining the organism (2). The purpose of this experiment was to identify the organism as gram-positive or gram-negative. The first step in the gram staining procedure

and Methods: Two unknowns where received by the instructor unknown K and C. using the methods learn in lab for identifying them. Beginning with a Nutrient Agar plate T streak to identify morphology and purity once completed and gram stain was performed. Once the gram stain was determined an oxidase test was performed on both organisms a tested used to determine if the bacterium produces cytochrome c oxidase. Thus moving into Gelatin hydrolysis testing a test preformed to identify if the microbes

and lastly ,a Gelatin test, the unknown bacterium was narrowed down to E. coli. Important conclusions of this experiment were that many tests can be used for identification besides a simple gram stain. Introduction There are several ways to identify unknown bacteria. The first way of identification is gram stains. When a cell is

enumerating Salmonella spp. in a food sample. 1.2 Hypothesis To achieve a negative results in food sample with the absence of Salmonella spp. 1.3 Introduction The genus Salmonella belong in the family of Enterobacteriaceae is a group of rod-shaped, Gram-negative, and facultative anaerobic bacteria, which grow with or without oxygen. It is also a non-spore forming and a motile or non-motile bacterium with exception of S.gallinarum and S.pullorum. The genus Salmonella consist of two species, S. enterica

Part A: Osmosis practical task Aim: To observe the effects of osmosis in rhubarb cells. Hypothesis: Water will be extracted out of the cells in the salt solution causing the cells to look different to the cells in the freshwater solution. Materials: Rhubarb Distilled water in a dropping bottle Salt solution in a dropping bottle Microscope, slides, and coverslips Forceps and razor blades or scalpel Paper Towel Method: Clean and dry a slide and coverslip. Obtain a small sample of the red epidermal

INTRODUCTION Bacterial diseases is any type of disease caused by bacteria. Bacteria 's are a kind of microorganism, which are tiny forms of life that cannot see by easy only be seen with a microscope. The bacteria that cause disease are called pathogenic bacteria. Bacterial diseases happen when pathogenic bacteria inter the body and begin to reproduce and grow in tissues. Pathogenic bacteria may also emit toxins that damage the body. Common pathogenic bacteria and the types of bacterial diseases

The career I have chosen is a microbiologist, some of the main characteristics of the job are working with research teams, and other scientists and technicians. Studying microorganisms like bacteria, viruses, algae, fungi, and several other parasites. They do their work, and studies to learn, or figure out how these organisms live, grow, and interact with their environments. Planning, and conducting complex research projects, you can develop new drugs to combat infectious diseases, and any infection

1. Describe the main characteristics of Alcaligenes faecalis. Where is this bacterium naturally found? How large is the genome of Alcaligenes faecalis? Alcaligenes faecalis is an aerobic nonfermentative, oxidase-positive, nonencapsulated, gram-negative rod. It is so named for its ability to produce an alkaline reaction in certain media. Microscopic analyses revealed that the bacteria are located in the esophagus and intestine of the nematodes Steinernema feltiae, S. carpocapsae, and H. bacteriophora

Yousef Hariri Professor Fowler Bio 10 September 24, 2017 Writing Assignment 1 1) A simple way to check whether the cells are bacterial, plant, or animal would be to examine the characteristics of a single cell. A big difference that bacterial cells have that is not found in plant or animal cells is a Nucleoid, which is a certain area whether the genome of the bacteria lies. To differentiate a plant cell from the other two types, one can look for either a cell wall and/or chloroplast, which is what

Name : Terry-Ann Poorman Id# : 27120454 Lab# : 1 Lab Instructor: Mrs. Bryan Title : Aim : Discussion Questions: 1) What are the types of urine specimens? Random specimen This type of specimen is used for microscopic and chemical analysis. As the name suggest this sample can be a randomly collected specimen and at an unspecified times. This is often more convenient for the patient. A random specimen is suitable for most screening purposes. Random specimens can at times give an inaccurate view of

the introduction were proven correct but most were not. In the beginning, it was thought that in three plates the bacteria could be separated, however, it actually took four plates to separate the bacteria. It was confirmed that both bacteria are gram positive. However, along with this one of my bacteria was not Staphylococcus. One being Bacillus was confirmed and actually both ended up being Bacillus species. It was thought that one bacteria would be catalase negative and the other catalase

animal glue, etc.) to achieve the adhesion between the particles and consequently to be applied as paint film on the dry ground. The pigment must be dispersed or grinded as evenly as possible in the binding medium to take full advantage of the properties of both the pigment and the binder.[8] The typical stratigraphic structure of the Egyptian wall paintings is usually made of multiple layers. The plastering procedure was to initially smooth an irregular rock surface with one or more of plaster layers

AMITY INSTITUTE oF MICRoBIAL TECHNoLoGY AMITY UNIVERSITY UTTAR PRADESH NTCC oN HANDS oN VARIoUS PATHoLoGICAL TEST Submitted to Dr. DEVENDRA KUMAR Amity Institute of Microbial Technology Guided By:DR.MoHAN MEHTA Submitted By -RISHIN GUPTA Enrolment. No. A0989216009 TABLE oF CoNTENT 1. Declaration by the student 2. Certificate by the faculty guide 3. Abstract 4. Intro 5. Experiments ABSTRACT Demonstrative testing in labs is of incredible esteem while evaluating a

The Unknown Identification Lab was an experiment that provided the opportunity to apply all the tests that were learned in the semester of lab, to identify the two bacterias that remain unknown. Gram- staining and two other tests will be used to identify the unknowns. This experiment is crucial to the understanding of each test, and can benefit in the ability to identify the characteristics of specific bacteria. Having a clearer understanding of the bacteria can further the research of bacteria for

I conducted were the Gram Stain Test, Endospore stain test, Urease test, Lactase test, and lastly the MRVP Methyl Red test. The first test conducted was the Gram Stain test. This test was used to determine if the two bacteria were either Gram Negative or Gram Positive. There are four main steps in the gram stain process that all affect the outcome and overall stain and accuracy of the test. The Primary stain is the first step in the gram stain. This process includes staining the cells, Negative

second ½ of the organism was used for gram staining. The gram stain method was performed on the unknown organism per lab manual page 42 and two gram stain reactions were identified. Organism B was gram positive cocci in grape like clusters. Because organism B was positive I could eliminate Escherichia coli, Enterobacter aerogenes and Proteus vulgaris because these bacteria would be rod shapes. Organism A was gram negative pink rod shaped and because of gram positive morphology I could eliminate