Urease Essays

noninvasive. The invasive tests including histology, urease tests and culture, require upper gastrointestinal endoscopy for obtaining the diagnostic sample. On the other hand, non-invasive methods include the urea breath test, serology and stool antigen test .(12) Invasive Methods : Rapid Urease Test The Rapid Urease Test (RUT) is a popular invasive diagnostic H. pylori test that is relatively quick, cheap and simple to perform. It detects the presence of urease in

The bacterium was determined to be Gram- negative rods. The first attempt of gram stain the unknown appeared to look positive and cocci. But, the next day onwards for 4 consecutive days the unknown showed up as a gram- negative gram stain and appeared pink rods. Triple Sugar Iron Agar is a differential medium. It differentiates Gram-negative enteric bacteria based on the ability to reduce sulfur and ferment carbohydrates (lactose, sucrose, and glucose). This is indicated by the formation of acid

Staphylococcus aureus Introduction/ Background information Staphylococcus aureus (S. aureus) is yellow-pigmented colonies and this is a reason for (aureus = golden) in its genus name. S. aureus is a spherical shape (Coccus), gram positive bacterium that usually present in pairs as seen in microscope.it has short chains, or bunched, grape-like clusters, non-motile, no spores and facultative anaerobic. Members of this species can survive in the aerobic or anaerobic conditions and they can adapt to

the main goal of this experiment is to identify the particular unknown bacteria. After doing all the biochemical tests, which includes Triple Sugar Iron Agar test, Sulfur Indole Motility test, Methyl Red test, Voges- Proskauer test, Citrate test, Urease test, and Gelatin test, which took 24 hours incubation time results proved that the given unknown gram negative bacteria was found to be Escherichia coli. II. Introduction In both Gram-negative and Gram-positive bacteria, the cell wall is constructed

experiment is to determine an unknown bacterium by applying several tests and comparing with known results. Through utilization of a Triple Sugar Iron Agar test, a Sulfate Indole Motility test, a Methyl Red and Vorges-Proskauer test, a Citrate test, a Urease Hydrolysis test, and lastly ,a Gelatin test, the unknown bacterium was narrowed down to E. coli. Important conclusions of this experiment were that many tests can be used for identification besides a simple gram stain. Introduction There are several

Prokaryotic organisms normally have a cytoplasmic membrane, cell wall, and sometimes a capsule. Bacterial cells are most commonly either coccus or bacillus in shape. The cell wall is either Gram positive or Gram negative. When the cell is Gram negative, the cell has an extra layer of lipopolysaccharides. The Gram positive has a thick layer of peptidoglycan. Bacteria usually have capsules, but archaea rarely have one. Inside the prokaryote is cytoplasm and a nucleoid. The nucleus is not enclosed inside

determine which of the following unknown bacteria was in test tube number 16: Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, and Salmonella typhimurium. Biochemical tests were used to help identify the unknown bacteria. The Citrate test, Urease test, Triple Sugar Iron Agar test, Voges-Proskauer test, and Methyl Red test were the biochemical tests used in this experiment. The T-Streak method was also used to ensure that pure colonies were created. The results of the biomedical test concluded

1. Describe the principal, advantage and limitation of every diagnostic test to detect H.pylori.(1, 2) Test Principal Advantage Limitation Rapid urease test In the presence of H. pylori urease, urea is metabolized to ammonia and bicarbonate resulting in an increase in pH, which changes the color of a pH-sensitive indicator. Tests for active H. pylori infection; >90% sensitivity and specificity. Results are rapid (within 24 hours), and test is less expensive than histology or culture. Withhold H2RAs

The first time the unknown bacteria was isolated, one of the colonies was observed to be bright yellow. This was important because it gave a clue as to which one was gram positive because looking for a yellow color was part of the gram positive dichotomous key. However, this conclusion that the yellow colony was positive and the white colony was negative was not certain until the conduction of the second gram stain. Also, even though Micrococcus was the only possible bacteria with colonies that have

TITLE: The Mating Of Different Strains of Saccharomyces Cerevisiae From Different Types of Fruits Samples INTRODUCTION: Saccharomyces cerevisiae is a yeast that are microorganism with a single cell. They have genetic content which is contained within a nucleus. This classified them as eucaryotic miccrorganism different with bacteria. Bacteria do not have nucleus and was classified as procaryotic organism. Yeast commonly found on flowers, soils, plants and also fruits. Yeast Saccharomyces cerevisiae

Materials Based off of the flow chart and the methods took to identify the two unknowns the tests I conducted were the Gram Stain Test, Endospore stain test, Urease test, Lactase test, and lastly the MRVP Methyl Red test. The first test conducted was the Gram Stain test. This test was used to determine if the two bacteria were either Gram Negative or Gram Positive. There are four main steps in the gram stain process that all affect the outcome and overall stain and accuracy of the test. The Primary

During a lab session an unknown bacteria, Unknown #57, was assigned to determine its Genus species. A series of tests were performed with subcultures made when needed and antiseptic techniques used throughout. How bacteria differ from each other chemically as well as their shape can be determined by a differential stain known as a Gram stain. Gram positive bacteria have thick cell walls made of many layers of peptidoglycan which enable the bacteria to retain the crystal violet stain. In contrast

Pylori, antibodies were found in the bloodstream. Helicobacter Pylori are able to attach to cells as they go through the mucous layer of the stomach. When the bacterium enters the stomach, an enzyme called urease converts the stomach cells chemical urea into ammonia and carbon dioxide. The enzyme urease tends to trigger inflammation. Once those chemicals have been converted, the acidity in the mucous surrounding the bacteria then becomes neutral protecting the bacteria. Even with the immune system being

Introduction: Microbiology is the study of the microbial world, which is composed of microorganisms that are too tiny to be seen with a naked eye. This project demonstrates the significance of correctly identifying unknown organisms with methods and techniques acquired in the laboratory as they can make or break one's ability to accurately isolate and identify various species. Studying microbes enables oneself to have an insight on not just their structure or how they function, but how they have

Introduction The purpose of this experiment was to apply the knowledge gained from this semester of microbiology. A single, unknown bacterium was provided on a nutrient agar plate. Several microbiological tests were performed using sterile techniques that were learned to determine the identity of the unknown. Tests Conducted The first step to identify the unknown was to isolate the bacteria that was provided. This was done using a streak method on a nutrient agar plate. After one week the bacteria

Following are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Beta glucouronidase test is used for the identification of Escherichia coli. An enzyme is produced by E.coli which is beta D glucouronidase. Beta d glucouronidase in turn hydrolyzes beta d glucopyranosid uronic derivatives to aglycons and D glucuronic acid. Bile solubility test is used in laboratory for differentiation of alpha hemolytic Streptococci

Urea cycle Introduction The task of urea cycle is to avoid the amassing of toxic NH4+. It incorporates nitrogen not used for biosynthetic purposes into urea, which serve as the waste nitrogen produce in mammals. Urea is the major disposal form of amino groups derived from amino acids, and account for about 90% of the nitrogen-containing gears of urine. Also urea cycle is accountable for de novo synthesis of arginine. The complete Urea Cycle is by far only in liver. However, some enzymes of the

Unknown Lab Report Unknown # 25 By: Jenna Riordan March 19, 2018 Bio 2843 1. Introduction Microbiology is the study of microorganisms found in all different environments throughout Earth, from the hot thermal vents at the bottom of the ocean to the ice at the top of a mountain. These microorganisms are used to teach us how multicellular organisms came to be and how they can survive today. These small, microscopic organisms are so unique that the identification of them is paramount in the advancements

Because of its important role in disease pathogenesis, the organism has been intensively studied. The H.Pylori genome encodes approximately 1500 proteins and contains 1.65million base pairs. H.Pylori expressses a number of novel proteins including urease. Urease

The medium had a pH indicator called phenol red and a potassium phosphate buffer that was used to resist alkalization of the medium from peptone metabolism. When the pH level elevated, rapid urease positive organisms turned a bright pink and orange or yellow colors indicated a negative result for urea hydrolysis. PHENYLALANINE Deaminase Test: This test looked for the enzyme, phenylalanine deaminase, which removed an NH2 group from cysteine