1. Introduction Liver disease represents a considerable public health burden that involves several etiologies overlapping symptoms (El-Serag & Rudolph, 2007). Since the seventies, the experimental model of carbon tetrachloride (CCl4) hepatotoxicity have been closely studied using in vivo models of acute and chronic liver injury. This process has been used comprehensively to examine the pathogenesis of liver injury such as centrilobular steatosis, inflammation, apoptosis and necrosis. CCl4 deteriorates hepatocytes directly by altering the permeability of the plasma, lysosomal, and mitochondrial membranes through initiation of lipid peroxidation with trichloromethyl radical (CCl3•) or trichloroperoxyl radical (CCl3OO•) by the hepatic microsomal cytochrome P450 oxygenase system. Oxidative stress and membrane damage in hepatocytes are mainly caused …show more content…
SOD activity was calculated in terms of units/mg protein. Total protein thiols (TPT) - This assay is based on the principle of formation of relatively stable yellow color by sulphydryl groups with DTNB (Moron et al., 1979). Briefly, 0.2 ml of liver homogenate was mixed with phosphate buffer (pH 8.0), 40 µl of 10 mM DTNB and 3.16 ml of methanol. This mixture was incubated for 10 min and the absorbance was measured at 412 nm against appropriate blanks. The total thiol content was calculated by using ε=13.6x10 cm-1 M-1 Sedlak & Lindsy, 1978). 2.11. Statistical analysis Data were analyzed using StatSoft STATISTICA version 12 software. Differences between the groups were assessed by a nonparametric Mann–Whitney and Kruskal–Wallis tests. Values in the text are means±standard deviation (SD). Differences with p0.05 were considered to be statistically
This value was 0.019589 and with this statistic we were able to look at our hypothesis again. We could successfully conclude that our null hypothesis of the urine having no effect on the mice was wrong. This is because of P-value is <0.05 and represents that are data was statistically
Lysosomes digest the toxins so they can be safely eliminated by the cell. Peroxisomes contain oxidases and catalases that detoxify substances like alcohol and neutralize free radicals. The smooth endoplasmic reticulum (ER) also contains enzymes that detoxify certain drugs (such as alcohol). 3. Mrs. Fender’s jaundice is caused by the accumulation of bilirubin in her blood and tissues.
At a level of significance of 0.05 the researcher is accepting a possible type I error 5 cases out of a 100 and 95 cases out of 100 a true null hypothesis (Loiselle et al., 2011). Step 3: Selection of one-tailed or two-tailed test The selection for a one-tailed or two-tailed test is not required for a Chi-Square for independence test (Salkind, 2013). Chi-Square test has both variables that are of a categorical level of measurement therefore, there is no direction (Salkind, 2013). Step 4:
Although the overall absorbance increases as more milliliters of mitochondrial suspension is added to a mixture of 0.25 mL of 0.5 mM DCIP, 0.5 mL of 50 mM sodium azide, various volumes of assay buffer (20 mM potassium
Discussions continued of Noseworthy 's comments, as the House Tax Committee chair, Rep. Ann Lenczewski, worked with Mayo Clinic and Rochester officials to revise their plan. The current proposal depends on slightly taking a part of future income, sales, income taxes, and property to pay for roads, bridges and other amenities in Rochester. However, the plan was not liked by Lenczewski, and he suggested Rochester and Olmsted County should go for more of the $585 million plan. Lenczewski mentioned that Mayo Clinic will get some of the money, but the large expansion would not be paid by the state. He also thought some members on the House side wanted to support something, but mainly wanted things to be done the right way.
Abstract The purpose of this experiment is to test for mitochondrial activity by isolating different organelles using the differential centrifugation process. Studying mitochondria is extremely important because they control the death and life of the cell by regulating the apoptotic signals (Frezza et al 2007). Also they are responsible for the metabolic reactions (aerobic respiration) and the production of ATP (Frezza et al 2007). Three hypotheses were formed based on my knowledge.
Starch solution is then placed into the test tube at a quantity of 5 mL. 5 drops of Lugol’s Iodine solution is added to the test tube. If the color changes, then it is known that starches are present in the solution. Proteins are next tested. In order to do this, 5 mL of gelatin solution is added to the test tube. 10 drops of Biuret’s reagent are added to test for protein.
Another proven false confession, Wayne and Sharmon Stock were found shot to death in an upstairs bedroom in April 17, 2006. Police had no leads, so they focused the investigation toward the family. More than a few family member suggested that Matthew Livers a 29 year-old, had a tough relationship with his Uncle Wayne and wasn’t much liked by his Aunt Sharmon. Authorities also connected Livers cousin Nicholas Sampson a 28 year-old with first-degree murder. During Livers interrogation by investigators from the Cass County Sheriff’s Office and Nebraska State Patrol for nearly 11 hour without a lawyer, put a lot of pressure towards Matthew to give the detective what they wanted.
1.1 Abstract The purpose of quantitative analysis of protein using a spectrophotometer is to measure the concentration of proteins in a given sample. The experiment is conducted by laboratory method (Biuret Test) and using spectrophotometer to analyze the absorbance of reactants at 540 nm, hence determining the concentration of the proteins in a given sample. The purpose of stopped enzyme assay to study B-galactosidase is to determine the effect of temperature and concentrations of substrate on enzyme activity.
The literature support for this project consists of analysis of several studies that examine the impact on access to care for those who live with a minimum of one health condition, and have limited income. Further, the studies examine these impacts on both those less than 65 years-old, and those 65 years-old and greater. Last, the impact of access to transportation is examined with regard to its impact on access to care (access). These issues are contrasted with how telemedicine can improve access for this patient population.
Proper sample size was used and the trial duration was long enough to capture the characteristics of
Snowflakes in the liver Case presentation: A 43-year-old male patient was admitted to the Emergency Department ward for general malaise with abdominal pain in the right upper quadrant, breath employee, already present two months before and actually increase. There was no sign of fever, pathologic lymphadenopathy, jaundice or other symptoms. At physical examination the patient appeared lucid, well spatial/temporal-oriented, afebrile, hemodynamically stable (AP 100/70 mmHg, HR 68 bpm), weight 73 Kg, height 180 cm; all examinations were in the normal range, except for mild deep pain in right upper quadrant and the right shoulder. Past History:
Introduction Living donor liver transplantation (LDLT) is a lifesaving alternative to deceased donor liver transplantation (DDLT) for patients with end stage liver disease. Best matching between graft size and recipient weight is crucial to avert small for size syndrome and its serious complications. Liver represents approximately 2% of the total body weight and liver graft required to meet metabolic demands should be 40-50% of liver weight which in parallel equals 0.8-1% of the recipient’s weight. This lead to the concept that graft to recipient weight ratio (GRWR) should be at least 0.8% for successful liver transplantation [1-9]. To obtain the desired GRWR of 0.8% or more, accurate estimation of graft weight is required.
PREVENTION AND CONTROL OF HEPATITIS B IN THE UNITED STATES Antony Van Leeuwenhoek’s development of the microscope in the 1600’s allowed scientists to visualize microorganisms that cause infectious diseases for the first time. (Paul g. Engelkirk –Janet Engelkirk 2011, Burton’s microbiology for health sciences. Ninth Edition p.7). Since then different bacteria and viruses have been discovered by use of a microscope such as Hepatitis viruses. There are five main recognized Hepatitis viruses that is , Hepatitis A (HAC), Hepatitis B(HBV) , Hepatitis c, Hepatitis D, Hepatitis E, Hepatitis
LIVER ANATOMY AND PHYSIOLOGY The liver is located in the region of the right upper quadrant of the abdomen (usually does not exceed the limit of the costal margin), filling the space of the diaphragmatic dome, where it can reach up to the fifth rib, and is related to the heart through the center phrenic, to the left of the inferior vena cava. It is usually soft and palpable, and is covered by a fibrous capsule. This fibrous capsule applies peritoneum mainsail liver surface (except liver bare area). Is divided into four lobes macroscopically: 1.