Solvent n-hexane, ethyl acetate and acetone will use to elute the column in isolation of chemical constituents of figeroots chloroform partition. The solvent system of different ratios of hexane, hexane/ethyl acetate, hexane/acetone, and acetone will use. Silica gel 60 (mesh 230-400 ASTM) will use to pack CC. In order to achieve good separation, mass ratio of silica gel to compound 20:1and 50:1 will use. 3.10.2Column Packing Before starting to pack a column, a small piece of cotton is gently will insert into the centre hole of column with the aid of a long stick. A small amount of n-hexane will add into the column. Silica gel weighted is mixed with n-hexane to form a mobile slurry solution and then slowly transferred into column. The side of the column will gently tape to prevent the formation of air bubbles in the column. Additional solvent will use to add the remaining silica gel into the column. Once all silica gel will add into column will gently tapped again to make the silica gel layer is flat. One precaution when packing a column is that never let the solvent to …show more content…
The sample will prepare by using the KBr pelleting method. The sample will mix with the KBr pellet in the mortar and pestle to grind until become fine powder form. The mixture will then press in a special die at 10,000 lb in-2 to yield a transparent disc. Before running the test of the sample, the background spectra will collected under identical conditions is subtracted from the sample spectra automatically. The spectrometer analysed the compound in the KBr disc form and a spectrum was displayed after the testing of the sample was completed. Based on the peaks resolved in the spectra, the possible IR functional groups which relevant to the chemical constituent isolated were identified by table of characteristic IR absorption (refer Appendix
Abstract: In this experiment, triphenylmethanol was synthesized in two steps. First, the bromobenzene was reacted with dry magnesium turnings to produce Grignard reagent. Second, the Grignard reagent was reacted with methyl benzoate and concentrated sulfuric acid to produce an alcohol. The end result of the experiment was not very successful because only 17% yield of final product triphenylmethanol was recovered, and the final product was impure based on the melting point and the IR spectrum results.
In this lab, the oxidation of a secondary alcohol was performed and analyzed. An environmentally friendly reagent, sodium hypochlorite, was used to oxidize the alcohol, and an IR spectrum was obtained in order to identify the starting compound and final product. The starting compound could have been one of four alcohols, cyclopentanol, cyclohexanol, 3-heptanol, or 2-heptanol. Since these were the only four initial compounds, the ketone obtained at the end of the experiment could only be one of four products, cyclopentanone, cyclohexanone, 3-heptanone, or 2-heptanone. In order to retrieve one of these ketones, first 1.75g of unknown D was obtained.
Empty the blank and use the solution from test tube one to rinse the cuvette twice. Fill it ¾ with solution one, wipe the outside, and place it in the spectrometer. 8. Start data collection and display a full spectrum graph. Stop the data and the wavelength of maximum absorbance will be identified.
On the IR spectrum of camphor, a carbonyl functional group (C=O) appeared at 1737 cm-1 with a very large peak. There was also two C-H stretches on the IR spectrum between 2873-2956 cm-1. The SDBS of the true camphor showed all of these same peaks around the same wavenumber. With this observation the oxidation of borneol to camphor was a success. On The IR spectrum of isoborneol, a carbonyl functional group also appeared around 1735 cm-1
Rule a line in pencil 2cm from the bottom end of the paper 3. Place a mark on the pencil line with two of the pens/textas 4. Add approximately 25mL water to the beaker 5. Sticky tape the filter pater to the pencil/popstick and suspend in the water as shown so that the water level is 1cm below the spot. 6.
In the round-bottom flask (100 mL), we placed p-aminobenzoic acid (1.2 g) and ethanol (12 mL). We swirled the mixture until the solid dissolved completely. We used Pasteur pipet to add concentrated sulfuric acid (1.0 mL) to the flask. We added boiling stone and assembled the reflux. Then, we did reflux for 75 minutes.
Pure ASA crystals are isolated from the solution with a Hirsch Funnel that was used with a filter. The melting point of the pure ASA crystals were calculated in order to calculate of absorbance. Iron (III) salicylate dianion must contain the acidified solution Fe3+ in order to measure the absorbance values. The level of the impurity can
Chem 51 LB Experiment 3 Report Scaffold: Bromination of Trans-Cinnamic Acid 1. The goal of this experiment was to perform a halogenation reaction through the addition of two bromides from pyridinium tribromide. This was accomplished by reacting trans-cinnamic acid with pyridinium tribromide. After the reaction took place, melting point analysis was conducted to find out the stereochemistry of the product, which could either be syn-addition, anti-addition, or syn + anti-addition. 2.
3. To purify and identify the product, recrystallization is used in order to purify the product, then melting point and TLC techniques are used to identify the product. Theory 4.
Abstract Heliox is a mixture of oxygen and helium in specific percentages, which is used in treatment of obstructive diseases (like asthma). It can be used in adults as well as in pediatrics for upper or lower airway diseases. It is almost safe and has no noticeable side effects. Introduction Heliox is a gas used in hospitals in order to help patients to breath. Heliox is composed of two gases which are helium and oxygen.
Introduction When a chemical reaction occurs anywhere in the universe, it needs energy. The human body is no exception. For some reactions, the energy required to start the reaction is Enzymes are special proteins designed to assist in the breaking down of macromolecules. They do so by holding the macromolecule in place at the active site, therefore lowering the amount of energy it takes to start the chemical reaction. There are different enzymes for each macromolecule; Pectinase and Cellulase are both examples of enzymes, and were the enzymes tested in this lab.
(Molarity)(Volume)(Molar mass) The pellets were dissolved thoroughly then was used in filling up the 100 mL volumetric flask. The solution was mixed well
A small amount of sand was added after the layer of cotton. After that, a layer of silica filled almost 1/3 of the column. Finally, another small amount of sand was added just above the silica. The column was given a little tap with an aspirator to make the silica more compact. Figure 2.
The other absorptions attributed to the molecule are shown in Table 5.4. Also the IR absorption peak can be compared as shown in Figure 5.3 and Figure 5.4. Table 5.4 Spectroscopic identification of erucic acid functional groups in FTIR Absorption frequency range(cm-1) Absorption peak (cm-1) Type of vibration 3020-3100 2920 =
FTIR Spectroscopic Study on Quantitation of Urea in Human BloodSerum Abstract: The quantitation of urea has been achieved using FTIR spectroscopy. The FTIR spectra of human blood serum samples are recorded in Mid IR region 4000-400 cm-1.The normal blood serum is treated with urea at different concentrations and FTIR spectra are recorded, which confirm the specific peaks related to urea. A plot between concentration of urea and percentage of absorbancehas shown linear relationship.