Addition Sequence B(ml) S(ml) T(ml)
Working reagent
Distilled water
Uric acid Standard (S)
Sample 1.0 1.0 1.0
0.02 -- --
-- 0.02 --
-- -- 0.02
Mix well and incubate at 37oC for 5 min. or at R.T. (25oC) for 15 min. Measure the absorbance of the standard (Abs.S), and Test Sample (Abs.T) against the Blank, within 30 min.
Calculations
Uric Acid in mg/dl = Abs.T X 8 Abs.S
2. MICROALBUMINURIA
Albumin refers generally to any protein that is water soluble, which is moderately soluble in concentrated salt solutions, and experiences heat coagulation (protein denaturation). They are unique
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They also serve as carriers for molecules of low water solubility this way isolating their hydrophobic nature, including lipid soluble hormones, bile salts, unconjugated bilirubin, free fatty acids (apoprotein), calcium, ions (transferrin), and some drugs like warfarin, phenobutazone, clofibrate & phenytoin. For this reason, it's sometimes referred as a molecular "taxi". Competition between drugs for albumin binding sites may cause drug interaction by increasing the free fraction of one of the drugs, thereby affecting …show more content…
Results were expressed in mean ± SD. Probability values of P < 0.05 were considered to be statistically significant. R2 value represents the correlation between the biological parameters. VI. RESULTS
The study was conducted on acute myocardial infarction patients admitted to the ICCU of MGM Hospital, Warangal.
Sample Description
The study groups included 50 patients of acute myocardial infarction in which 32 patients are AMI, 4 patients are AMI with Diabetes mellitus, 8 patients are AMI with Hypertension and 6 patients are AMI with Diabetes and Hypertension respectively. Hence AMI patients (n=32) with no co morbidities were included in the study with a mean age of 54.03 ±13.30 years. The gender distribution was found to be 24 males and 8 females. The control group consisted of 27 males and 5 females with a mean age 52.28 ± 14.14 years.
Blood Pressure
Systolic (‘p’ value 0.0875) and diastolic blood pressure (‘p’ value 0.1247) was significantly high in AMI as compared with control.
The age and gender distribution are presented in Table 1.
Age in years Control (n=32) AMI (n=32) Male Female Male