Catalase Activity on Substrate Based On Gas Pressure Production Rate Name of the Class Author’s Name Date Enzymes are organic compounds which act as catalysts and speed up biological reactions in biological organisms. They are not destroyed or changed during the reaction but rather they are used over and over again to catalyze many more reactions. Their activity may be affected and altered by factors such as temperature, substrate concentration, enzyme concentration and Ph.
The objective of this lab was to determine the best pH level to increase enzyme activity. As this objective was met, it was discovered that water (pH level 7) was the best for percent absorbance. The hypothesis for this experiment was, “If peroxidase is an enzyme and therefore contains certain pH tolerances, then when placed in solution with pH levels of three, seven, and ten and the reaction is measured by a colorimeter, then water will be the optimal solution for maximum reaction rate.” As seen in the tables and graphs, the data supported the hypothesis due to the fact that most enzymes have an optimal pH of 4-9.
The effect of pH on the speed of enzyme interaction with substrate chemicals Hypothesis: About pH: If the pH level is less than 5, then the speed of the enzyme reaction will be slower. About temperature: If the temperature stays the same, then the speed of the enzyme reaction will not be completely affected. Background information: The function of enzymes is to speed up the biochemical reaction by lowering the activation energy, they do this by colliding with the substrate.
DIY - What Is Life? How can you determine whether something is alive, dead, or non-living? Whenever we speak of life, we must think in terms of cells.
It was hypothesized that the optimal pH for the enzyme was pH 7 while the 1.0 ml peroxidase would have the best reaction rate. At the end of the experiment the results prove the hypothesis to be incorrect. INTRODUCTION Enzymes are proteins that allow a reaction to speed up. These proteins are made up of monomers known as amino acids.
To begin, in this lab we studied the structures of leaves under a microscope. A leaf is best described as a major photosynthetic organ of green plants. Plants carry out the process of photosynthesis to convert carbon dioxide and water into oxygen and glucose for usable energy. This can be represented in a general equation which is 6CO2 + H2O -------> (sunlight and chlorophyll) C6 H12 O6 +6O2. Initially, the leaf is protected by the upper and lower epidermis as well as the cuticle.
Introduction: Enzymes are needed for survival in any living system and they control cellular reactions. Enzymes speed up chemical reactions by lowering the energy needed for molecules to begin reacting with each other. They do this by forming an enzyme-substrate complex that reduces energy that is required for a specific reaction to occur. Enzymes determine their functions by their shape and structure. Enzymes are made of amino acids, it 's made of anywhere from a hundred to a million amino acids, each they are bonded to other chemical bonds.
H20 + 2 O2 This experiment will use 1% catalase solution and 3% hydrogen peroxide solution, both diluted into water so the reaction slows down. Temperature will be controlled in this experiment to change the reaction speed of the enzyme and the substrate, this is what the experiment is looking at. The effect of the temperature will be determined by how much gas is released in two minutes, which will change the pressure inside the test tube and will be measured by a gas
Title The purpose of this experiment was to test the reaction rate of an enzyme in various temperatures to further learn what such enzyme’s (phosphatase) optimum temperature is. By learning what the optimum temperature is, we can hypothesize what type of environment the specimen in which the enzyme was isolated from lives in. In this experiment, therefore, the independent variable was the temperature in which the reactions took place. The dependent variable was the rate of the reactions measured by the light absorbance of the product (p-nitrophenol).
Introduction In class, a series of experiments were performed that pertained to the enzyme known as catalase, which converts hydrogen peroxide into oxygen. Due to peroxide being toxic to the tissues of both plants and animals, both possess the enzyme catalase, which breaks into two non-toxic compounds: water and oxygen gas. Enzymes are proteins that react to certain substrates to create a product, and continue doing so afterwards. Methods and Materials To test reactions between catalase and hydrogen peroxide, groups of three to four people were formed.
ABSTRACT: The purpose of the experiments for week 5 and week 6 support each other in the further understanding of enzyme reactions. During week 5, the effects of a substrate and enzyme concentration on enzyme reaction rate was observed. Week 6, the effects of temperature and inhibitor on a reaction rate were monitored. For testing the effects of concentrations, we needed to use the table that was used in week 3, Cells.
Describe your prior research experience. (500 words or less) I first became involved in scientific research during my first semester at Georgetown by joining the lab of Professor Timothy Warren. I investigated ammonia’s viability as a sustainable, carbon-free fuel source by building, designing, and characterizing novel iron catalysts that could perform ammonia oxidation. I was immediately intrigued by the thrill of science: the glove boxes I learned to spend hours working in, the vibrant purple compounds I would synthesize, and the x-ray crystal structures I obtained to see the invisible.
My interest in Biochemistry came to fruition when my father sustained a severe injury. Fortunately his leg healed to a point he could use it. However longer than 10 minutes of pressure caused immense agony as the injury had an impact on his nervous system. He was admitted to pain management and over a period of 6 years, he was given a wide range of medicine from Pregabalin to Nortripyline to help subdue the discomfort. These drugs were purely masking and helping to manage the pain, but as with all medication, after prolonged use his body became immune to them.
My fascination around biochemistry grew after witnessing my mother overcome breast cancer. It was since then I had become curious into learning more about the disease and the ways in which we can treat it. Based on the fact that the origin of the disease lies in genetics and the biochemistry of living cells, I believe my chosen degree will aid me to understand more on this inspiring field of study. The subject explains the complexity of life to a molecular level and holds the answers to the most fascinating and compelling questions about the origin of life. As a prospective scientist, I hope that I can contribute to the advances of scientific research, by providing cutting-edge and constructive theories that could potentially hold the answers that we seek in making new discoveries.
INTRODUCTION: Good Laboratory Practice is a quality standard where there is an implementation of designed laboratory studies and this is reported to ensure the result is uniform, consistent, reliable and also reproducible with quality and integration of chemical nonclinical tests of safety. There are proper procedures and protocols in GLP which are to be implemented by the laboratory GLP includes following: 1. Good laboratory management (including quality management) 2. Improved efficiency (cost reduction) 3.
