ipl-logo

Extraction Of Β-Caryophyllene Lab Report

994 Words4 Pages

Extraction of β-caryophyllene
Ground cloves will be used to isolate β-caryophyllene via steam distillation. 5g of ground cloves will be taken in a 500mL RBF(Round bottom flask) with boiling stones, 40mL of dH2O, and 3 to 4 drops of an antifoaming agent (to prevent violent boiling). Then, the contents of the flask will be heated on a heating mantle for an hour and followed by condensation of the distillate through a water jacket. Then allow it to collect in a graduated cylinder. Steam distillation will allow the clove oil to co-distill with the water, which take place at a least temperature than the boiling temperature of the individual solutions. This is desirable, because the components of clove oils get decompose at high temperatures. Then …show more content…

500gms of fresh beetroot or its hairy root has to be homogenized with sand continuously for three times in 70% ethanol (100mg/L).Then extracts will be centrifuged at 10xg for half an hour and supernatants will be allowed to vaporise at 40°C under vacuum till they get dried. Then, the residues will be dissolve in 0.5L of 70% of methanol and this sample-methanol mixtures keep in refrigerator at -20 degrees for 24hr, consequently thawed and supernatants are carefully collected from the precipitate. Under vacuum, methanol will be evaporated, at 40°C, from the supernatants and following betalains are lyophilized from aqueous fractions and finally 6.5gms of dry betalains will be obtained (Christ, Alpha 1-2, …show more content…

Exponentially growing cells, 1x104, will be added to each well in 96 well plate and followed by treatment with different concentrations of drug and allow them to incubation for some period. Upon addition of MTT, the plates will be allowed for incubation at 37°C for 4 h. Then the samples will be read at 570 nm, upon dissolution of formazan salt in DMSO to calculate the 50% inhibitory concentration (IC50) of drug.
b. Trypan Blue exclusion technique By Trypan Blue exclusion technique, the effect of drug on the MCF-7 Cancer cell will be determined. Briefly, 3x104 cells will be added to each well in 24 well plates and then expose with different concentrations of drug. Then plates will be allowed for incubation at 37°C and by using hemocytometer, after staining with 0.4% Trypan Blue every 24 h, number of cultured cells in the different wells will be counted to calculate the doubling time.
d. Cell viability in presence of caspase-3 inhibitor
The viability of MCF-7 cells treated with caspase-3 inhibitor and evaluation of drug will be done by using MTT assay. After incubation with caspase-3 inhibitor for 1 h, cells will be treated with different concentrations of drug(s) for upto 48 h after which viability will be

Show More
Related
215 Words | 1 Pages

In vitro: Treatment of MM cells with SRT1720 inhibited growth and induced apoptosis in MM cells resistant to bortezomib therapy without significantly affecting the viability of normal cells. Mechanistic studies demonstrated that anti-MM activity of SRT1720 is associated with activation of caspase-3, caspase-8, caspase-9, poly(ADP) ribose

Read More
338 Words | 2 Pages

This proves that chemical stress affecting the cell membrane of the beet was caused by the adding of ethanol in higher concentrations because the alcohol affects the cell membrane by attaching the cell membrane like water does cause it the changing shape and create a hole big enough for more alcohol to get in. This also causes alcohol to damage the vacuole causing the betacyanin to get released and the red pigment to get transferred into the ethanol around the beet. In conclusion, the chemical stress affected the cell membrane of a beet cell, because of the higher amount of ethanol added to the beet caused the cell membrane to change shape which resulted in the betacyanin being released from the cells. These results affect me because I have always wanted to understand why anything that the beets get mixed with, food, water, bowls, etc, will turn

Read More
976 Words | 4 Pages

In the late 1940s, scientific research began taking off as innovative technologies and diseases were being created and discovered. One important field of study during the time was cancer. Like many types of new research, there were a few problems getting the ball on the roll. One problem scientists faced was obtaining cancerous cells that would stay long enough to study. One scientist struggled with this until a particularly unique strand of cells came along.

Read More
426 Words | 2 Pages

To add to this, the Morphogenic Field Technique claims, “Beets are super-absorbers. If it is nutritious, the beet will absorb it. If it is toxic, the beet will also absorb those things as well. The result: A toxic, genetically engineered beet, growing in toxic soil and eventually turned into a toxic product called white sugar.” This concludes that sugar beets will absorb any noxious chemicals around them and this results in them becoming highly poisonous to

Read More
1346 Words | 6 Pages

INTRODUCTION This assignment is about the study of the effect of agonist and different concentration on guinea pig ileum and it will consist of method, graph results and discussion. Drug is defined as a chemical that has both biological and pharmacological effects on human. Its branch is pharmacology which can be divided into two branches namely pharmacodynamics and pharmaco kinetics. (C. Stephen and W. Robin (2010)) Pharmaco dynamic is about what drug does to the body and pharmaco kinetics is the study of what the body does to the drug.

Read More
825 Words | 4 Pages

Identification of Unknown Solutions and Ammonium Salts preAice Chemistry Lab Report Descriptions of unknown solutions: Unknown Descriptions A Clear, colorless, odorless liquid. B Clear, colorless, odorless liquid. C Clear, red-brown, odorless liquid D Clear, yellow-orange, odorless liquid.

Read More
883 Words | 4 Pages

In this experiment, beets will be a replica to explore membrane using various extremely low and high temperatures. The roots of beet contain an immense quantity of a reddish color called betacyanin that is located inside the central vacuoles, which are surrounded by a vacuolar membrane called the tonoplast (Biology Lab Manual, 2011). The protein structure of beetroot is very important, because once its interrupted it could lose its purpose and would have to experience denaturation. Cell membranes defend and organize cells, they serve as a fence which means that a few particles can disperse across the lipid bilayer but others cannot. The structure of the cell membrane contains

Read More
1607 Words | 7 Pages

According to Gerber (2008, p.312) the drugs involved in targeted therapy works initially by preventing the growth of cancerous cells. Targeted therapy interrupts the specific molecules which are required for growth and development of a tumor. It blocks the proliferation of cancer cells. Gerber’s (2008, p.311) studies show that the targeted therapy drugs are used to treat many malignancies including breast, colorectal, lung and pancreatic cancers as well as lymphoma, leukemia and multiple myelomas. The main two types of targeted therapy include monoclonal antibodies and small molecule inhibitors.

Read More
1035 Words | 5 Pages

“Insertion of IPTEN gene in Cowdin Syndrome’s patients’’ (A step towards Breast Cancer Control) Synopsis Submitted to: DR. HAMID MANZOOR Submitted by:

Read More
1000 Words | 4 Pages

Conclusion and Recommendations Ultimately, testing cell viability and knowing the count of viable and non-viable cells in a cell line is an important key in any research using cells. Having enough number of viable cells in a suspension would give accurate results in an experiment. This also shows how trypan blue is an important dye in cell viability and in the study of cells. This dye makes researcher’s life convenient in identifying and differentiating viable versus non-viable cells or live versus dead cells. It is really needed that researchers should ensure new and fresh cell cultures to ensure more viable cells in a culture.

Read More
1302 Words | 6 Pages

Various assays have been devised to evaluate apoptosis at several points of the cascade. Based on the methodology, the commonly used assays can be classified into the following groups: 1. Changes in cell morphology: morphological changes such as cell shrinkage, membrane blebbing, nuclear condensation, DNA fragmentation and changes in plasma membrane occur. Each of these changes can be quantified using Flow Cytometry. For example, the forward scatter parameter reduces on cell shrinkage while nuclear condensation causes an increase in side scatter.

Read More
1468 Words | 6 Pages

Clove oil has been found to be the strongest anti-oxidant of any essential oil, and is a component of ‘longevity’ formulas. It is also an extremely potent antibacterial, effective against a broader range of microbes than any other oil except perhaps Oregano – Clove oil has even been employed to sterilize surgical instruments. Clove also has analgesic properties, and can be used to temporarily reduce the pain of toothache. Clove oil (or ground cloves) is also a component of Dr. Hulda Clark’s anti-parasite protocol, helping eliminate parasites from one’s digestive system. This is a very powerful oil which should be diluted to 1% or less for topical

Read More
1489 Words | 6 Pages

Ali Atwi : Internal assesment – calculating of the concentration of ethanoic acid in vinegar AIM : To calculate the concentration of ethanoic acid CH3COOH in vinegar using stoichiometric equations, ( Yamaha brand ) Introduction : I personally like to add a little bit of vinegar on my food because it makes it taste better, yet I know that vinegar contains acid, and I also know the consequences of highly concentrated acid intake, like severe itching and stomach ache, vomiting. Venigar contains a small percentage of ethanoic acid Ch3COOH. This practical aims to find out the concentration of the of the vinegar against a standard solution of sodium hydroxide soloution of concentration 0.1 mol dm3 through acid-base titration, the label on the bottle says 6%.

Read More
1621 Words | 7 Pages

Methanol, has a molecule containing CH3OH it being the smallest, ethanol having more carbons and hydrogens than Methanol comes second, CH3CH2OH, and propanol, CH3CH2CH2OH, is the largest of the three molecules. Aim: The aim of this experiment is to see the effects of different alcohols and concentrations on the biological membrane of the beetroot. Research Question: How do different alcohols and concentrations affect the biological membrane of a beetroot? Hypothesis: As the alcohols increase in toxicity and concentration, the damaging and breaking down of the membrane will increase leading to more red pigment being released into the solution causing the absorption of the solute to increase.

Read More
4486 Words | 18 Pages

- Angiopoietin-2(Ang-2) ,Ang ( angiogenin) , epidermal-derived factor(EGF) , placental growth factor(PGF) , vascular endothelial-derived factor(VEGF) ,VEGFR and NRP-1, TGFα or β (growth-modifying factor) ,basic or acidic fibroblast growth factor (aFGFand bFGF) , platelet-derived growth factor (PDGFR and PDGF) , a clone stimulating factor CSF(G-CSF),Endothelin-1,tumor necrosis factor(TNFα) ,Integrin, Eph Receptors and Ephrin Ligands, Cyclooxygenase-2 and nitric oxide synthase(Cox-2-and eNOS) , bacterial lipopolysaccharide (LPS) , transmitted by hypoxia inducible factor (HIF-1) , hepatocyte growth factor and scatter factor (HGF / SF) , Erythropoietin (EPO) , Cathepsin B ,cathepsin S, Thrombopoietin (TPO) ,monocyte chemotaxis protein(MCP-1) , Histamine, plasminogen activators , Plasminogen activator inhibitor ,AC133 Id1/Id3,VE-cadherin and CD31

Read More

More about Extraction Of Β-Caryophyllene Lab Report

Related Topics

Open Document