Deep Oceanic Trenches

A major US oceanographic survey has just discovered new forms of life in one of the deep oceanic trenches of the world. Much remains undiscovered in deep ocean trenches and as a result, many scientists are currently researching oceanic trenches, more specifically deep-ocean chemosynthetic research (Vanreusel et al., 2004). The goal for many scientists lies in trying to determine what organisms exist in oceanic trenches, since not nearly all organisms that live down there have been discovered. The discoveries of life that can be made through investigation of the deep oceanic trenches in our seas and thus the discoveries that can be made about deep-sea ecosystems are very important. Research in this particular area is of great importance due

Thousands of grab samples were taken from nearby areas to determine if they contain evidence of life forms. In order to carry this task out, operational definitions of alive, dead, organic, and inorganic must be created. In order to assess these characteristics, several different tests will be performed including Benedict’s test, Biuret’s test, the iodine test, lipid test, Tetrazolium test, as well as the Feulgen test. In this experimentation, one contract will be developed in order to secure the initial analysis of the samples. It’s important to develop a specific, accurate, and cost effective method in order to analyze the samples given due to the fact that not all of the samples may be studied due to financial limitations and constraints. An accurate, specific, and cost effective proposal will allow one to determine which samples are most likely to contain evidence of life forms and thus which samples are most worthy of further

Each sample will undergo a series of tests in order to classify it as alive, dead (once alive), organic, and/or inorganic. In order to experimentally determine the nature of these various samples, operational definitions had to be created in order to accurately classify the samples based on the experimental results. The operational definition for alive will be a positive result in the Feulgen test as well as a positive result in the Tetrazolium test. The operational definition for dead will be a positive result in the Feulgen test and a negative result in the Tetrazolium test. The operational definition for organic will be is a negative result for the Feulgen test and a positive result in any of the macromolecule tests stated above. Lastly, the operational definition for inorganic will be a negative result for the Feulgen test as well as a negative result in all of the macromolecule tests. In regards to experimental design, one can see from figure one that experimentation will first begin by conducting a Feulgen test, which tests for the presence of DNA. A positive test indicates that the sample is either alive or dead whereas a negative test indicates that the sample is organic or inorganic. From this point after conducting the Feulgen test, one will conduct a Tetrazolium test for those samples that tested positive for the Feulgen test. This particular test tests for